RESUMO
Aim: To study the behavior of Candida albicans in women with vulvovaginal candidiasis (VVC), recurrent VVC (RVVC) and asymptomatic (AS), regarding adhesion on HeLa cells and their ability to express secreted aspartic proteinases (SAP) genes, agglutinin-like sequence (ALS) genes and HWP1. Materials & methods: The adhesion of Candida albicans to HeLa cells was evaluated by colony-forming units, and the expressed genes were evaluated by qRT-PCR. Results: AS and VVC isolates showed greater ability to adhere HeLa cells when compared with RVVC isolate. Nevertheless, RVVC isolate exhibited upregulation of a large number of genes of ALS and SAP gene families and HWP1 gene. Conclusion: The results demonstrated that RVVC isolate expressed significantly important genes for invasion and yeast-host interactions.
Assuntos
Ácido Aspártico Proteases/metabolismo , Candida albicans/genética , Candidíase Vulvovaginal/microbiologia , Ácido Aspártico Proteases/genética , Candida albicans/enzimologia , Candida albicans/crescimento & desenvolvimento , Colo do Útero/microbiologia , Feminino , Proteínas Fúngicas/genética , Proteínas Fúngicas/metabolismo , Regulação Fúngica da Expressão Gênica , Células HeLa , HumanosRESUMO
AIM: The present study evaluated the capacity of three species of Fusarium isolated from onychomycosis to form biofilms and the antibiofilm effect of propolis extract on these biofilms. MATERIALS & METHODS: The biofilms and antibiofilm effects were evaluated by quantifying the colony-forming units, mitochondrial metabolic activity assays, total biomass by crystal violet staining and scanning electron microscopy. RESULTS: Propolis extract demonstrated significant antibiofilm efficiency on Fusarium spp. isolates and reduced F. solani, F. oxysporum and F. subglutinans mature biofilms. CONCLUSION: Propolis extract can be an alternative topical treatment of onychomycosis caused by Fusarium spp.
Assuntos
Antifúngicos/farmacologia , Biofilmes/efeitos dos fármacos , Fusarium/efeitos dos fármacos , Dermatoses da Mão/tratamento farmacológico , Onicomicose/tratamento farmacológico , Própole/farmacologia , Antifúngicos/uso terapêutico , Fusarium/isolamento & purificação , Fusarium/fisiologia , Dermatoses da Mão/microbiologia , Humanos , Testes de Sensibilidade Microbiana , Microscopia de Fluorescência , Onicomicose/microbiologia , Própole/uso terapêuticoRESUMO
AIM: To evaluate the effect of propolis against Candida species planktonic cells and its counterpart's biofilms. MATERIALS & METHODS: The MIC values, time-kill curves and filamentation form inhibition were determined in Candida planktonic cells. The effect of propolis on Candida biofilms was assessed through quantification of CFUs. RESULTS: MIC values, ranging from 220 to 880 µg/ml, demonstrated higher efficiency on C. albicans and C. parapsilosis than on C. tropicalis cells. In addition, propolis was able to prevent Candida species biofilm's formation and eradicate their mature biofilms, coupled with a significant reduction on C. tropicalis and C. albicans filamentation. CONCLUSION: Propolis is an inhibitor of Candida virulence factors and represents an innovative alternative to fight candidiasis.