Adsorptive properties of graphene oxide on vitamin B12 and their effect on the promotion of peripheral nerve regeneration.

OBJECTIVES: To observe whether Graphene oxide (GO) can absorb vitamin B12 (VB12) and Decellularized scaffold - acellular nerve allograft (ANA) modified GO-VB12 promote the repair of ischiadic nervus defects in a rat model. METHODS: The adsorption of GO on vitamin and the optimum adsorption conditions were investigated by single factor experiment. The adsorption properties of the material were observed by scanning electron microscopy (SEM) and energy dispersive spectrometer (EDS) to determine the success of adsorption on VB12. GO-VB12-ANA was prepared by vibration mixing method and bridged to injured ischiadic nervus. The nerve action potential, wet weight ratio of gastrocnemius muscle and the expression of GAP-43 were investigated by contrast test to detect its effect on nerve regeneration. RESULTS: The optimized adsorption conditions for GO on VB12 solution were listed as follows: adsorbent dosage was 6 mg, shaking time was 70 min, the pH value was 6, the optimum concentration of VB12 was 50 mg/L and the theoretical saturated adsorption capacity was 21.51 mg/g. The nerve action potential, wet weight ratio of gastrocnemius muscle and the expression of GAP-43 in nerve fiber of GO-VB12-ANA group were close to the normal values and significantly higher than those of ANA and rotation group. CONCLUSIONS: Based on the adsorption function of GO on VB12, GO-VB12-ANA can promote regeneration of injured ischiadic nervus, providing the experimental basis for the clinical application of nanomaterials.

Inhibition of glucose-transporter 1 (GLUT-1) expression reversed Warburg effect in gastric cancer cell MKN45.

Glucose transporter-1 (GLUT-1) plays critical roles in cancer development and progression. Warburg effect (aerobic glycolysis) contributes greatly to tumorigenesis and could be targeted for tumor therapy. However, published data on the relationship between GLUT-1 and Warburg effect are scarce. In this study, gastric cancer cell, MKN45, was transfected with GLUT-1 shRNA using Lipofectamine 2000. Oxygen consumption, LDH activity, lactate production and cytoplasmic pyruvate were detected after MKN45 cells with GLUT-1 knockdown. In the last, hexokinase 1 (HK1), HK2, and pyruvate kinase M2 (PKM2) expression were detected by using western blot. In this study, we showed that inhibition of GLUT-1 expression reversed Warburg effect in MKN45 cells, and induced apoptosis.