RESUMO
OBJECTIVES: To compare self-collected vaginal swab (SCVS) types and first-catch urine (FCU) to diagnose Trichomonas vaginalis using analyte-specific reagents designed to be used in a transcription-mediated amplification assay. METHODS: A total of 241 women (group A) collected a FCU and a SCVS using a dacron swab (APTIMA collection kit). A second group of 289 women (group B) collected two SCVS using one dacron swab and one nylon-flocked swab. RESULTS: Of 75 young women (street youth) determined to be infected with T vaginalis only seven reported symptoms of vaginal discharge or irritation. Using a cutoff of 50,000 relative light units, the sensitivity and specificity was 97.2% and 97.6%, respectively for dacron SCVS compared with 41.7% and 100% for FCU in group A; 92.3% and 98.8% for dacron SCVS and 92.3% and 99.2% for flocked-nylon SCVS in group B. The assay tested 96 samples in 6 h. CONCLUSIONS: Dacron and nylon-flocked SCVS performed equally well and significantly better than FCU using analyte-specific reagents in the APTIMA transcription-mediated amplification assay. Either swab type could be used for self-collection.
Assuntos
DNA de Protozoário/genética , Autoexame/métodos , Manejo de Espécimes/métodos , Vaginite por Trichomonas/diagnóstico , Trichomonas vaginalis/isolamento & purificação , Urina/parasitologia , Vagina/parasitologia , Feminino , Humanos , Técnicas de Diagnóstico Molecular/métodos , Técnicas de Amplificação de Ácido Nucleico/métodos , Nylons , Parasitologia/métodos , Polietilenotereftalatos , Sensibilidade e Especificidade , Vaginite por Trichomonas/parasitologia , Trichomonas vaginalis/genética , Adulto JovemRESUMO
Aptima Combo 2 (AC2) Neisseria gonorrhoeae testing of 81,405 patients who were tested by culture and 14,666 who were AC2 tested for Chlamydia trachomatis detected 142 extra infections and confirmed 106 culture-positive samples (the positivity rate increased from 0.13 in testing by culture to 0.26 in testing by AC2). Retrievable AC2 positive samples were confirmed (98.5%) by an alternate AGC test.
Assuntos
Gonorreia/diagnóstico , Técnicas de Diagnóstico Molecular/métodos , Neisseria gonorrhoeae/isolamento & purificação , Infecções por Chlamydia/diagnóstico , Chlamydia trachomatis/genética , Chlamydia trachomatis/isolamento & purificação , Feminino , Humanos , Masculino , Neisseria gonorrhoeae/genética , Técnicas de Amplificação de Ácido Nucleico/métodos , Sensibilidade e EspecificidadeRESUMO
Tomatoes are an important source of antioxidant compounds, such as lycopene, phenolics and ascorbic acid. The main objective of this study was to determine the accessibility (availability for absorption) of the antioxidant compounds (total phenolics, total flavonoids, lycopene, ascorbic acid) and the antioxidant activity in fresh tomatoes of three cultivars (Excell, Tradiro and Flavourine) grown in New Zealand. The tomatoes were subjected to an in vitro digestion method, in which the pH, temperature, enzymes and chemical conditions were maintained according to human gastrointestinal conditions. The results showed that a high amount of the total phenolics and total flavonoids (71-77%) were released from tomatoes during digestion. However, only 3.2-4.5% of the total lycopene was released. No ascorbic acid could be detected after completion of in vitro digestion, probably due to degradation. After completion of digestion, the in vitro digestion extracts were found to have 45-50% antioxidant activity compared with the total antioxidant activity of fresh tomatoes (as measured by the 2,2'-azinobis (3-ethylbenzothiazoline-6-sulfonic acid diammonium salt radical decolourization assay). This study shows that the antioxidant components of tomatoes were affected by the in vitro digestion conditions depending on the type of compound. This in vitro digestion method gives an estimate of the release of antioxidant components in tomato, which may predict their in vivo behaviour.
