The First Occurrence of Hepatitis-Hydropericardium Syndrome in Iran and Effective Applied Control Measures in the Affected Commercial Broiler Flock.

Fowl adenoviruses cause three economically important diseases in broiler chicken flocks: hepatitis-hydropericardium syndrome (HHS), inclusion body hepatitis (IBH), and adenoviral gizzard erosion. IBH has not been considered a serious threat in northeast Iran since the last decade, because no major effect on flock performance has been noticed along with a low mortality rate. During this period, all the sporadic IBH outbreaks have also been investigated for HHS without finding any confirmed case. In March 2021, a 15-day-old commercial broiler flock in northeast Iran showed a 50% mortality rate, and birds underwent postmortem examination, histopathology, molecular testing, and phylogenetic analysis for possible disease agents. Typical gross lesions of HHS were observed postmortem that included hydropericardium with an unusual accumulation of jelly-like and straw-colored fluid in the pericardial sac (without right ventricular failure); petechial or ecchymotic hemorrhages on the myocardium, myocardial valves, and endocardium; and discolored and mottled liver along with small white foci and petechial or ecchymotic hemorrhages. Histopathologic analysis showed necrosis of hepatocytes and basophilic inclusion bodies in the livers. The molecular tests performed for detection of fowl adenovirus (FAdV), H5 avian influenza virus, Newcastle disease virus, avian infectious bronchitis virus (IBV), H9N2, chicken infectious anemia virus (CIAV), infectious bursal disease (IBD) virus, Marek's disease virus, Ornithobacterium rhinotracheale, Mycoplasma gallisepticum, and Mycoplasma synoviae turned out positive for FAdV, CIAV, IBD vaccine virus, and IBV serotypes 793B and variant I. The phylogenetic tree based on the hexon gene loop 1 demonstrated a FAdV serotype 4 (FAdV-4) that was identical to Pakistani isolate PARC-1/98. Because it was the first detection of a FAdV-4 in Iran, the stamping out program was applied immediately on the basis of HHS gross lesions and positive PCR reaction on pericardial jelly-like fluid. It seems that this eradication strategy was successful because no outbreaks were noticed for 2 mo after the initial outbreak. It was concluded that the use of gross pathologic baselines, quick diagnosis of disease, and close collaboration between governmental and private sectors were the critical factors that helped locally control the first occurrence of HHS in Iran.

Reporte de caso- Primera presentación del síndrome de hepatitis-hidropericardio (HHS) en Irán y medidas efectivas de control aplicadas en la parvada comercial de pollos de engorde afectada. Los adenovirus del pollo causan tres enfermedades económicamente importantes en las parvadas de pollos de engorde: síndrome de hepatitis-hidropericardio (HHS), hepatitis con cuerpos de inclusión (IBH) y erosión de la molleja por adenovirus. La hepatitis con cuerpos de inclusión no se ha considerado una amenaza grave en el noreste de Irán desde la última década, porque no se ha observado un efecto importante en el rendimiento de la parvada junto con una baja tasa de mortalidad. Durante este período, todos los brotes esporádicos de hepatitis con cuerpos de inclusión también han sido analizados para el síndrome de hepatitis-hidropericardio sin encontrar ningún caso confirmado. En marzo del 2021, una parvada comercial de pollos de engorde de 15 días de edad en el noreste de Irán mostró una tasa de mortalidad del 50 % y las aves se sometieron a un examen post mortem, histopatología, pruebas moleculares y análisis filogenético para detectar posibles agentes patógenos. Se observaron lesiones macroscópicas post mortem típicas del síndrome de hepatitis-hidropericardio que incluían hidropericardio con una acumulación inusual de líquido gelatinoso y de color pajizo en el saco pericárdico (sin insuficiencia ventricular derecha); hemorragias petequiales o equimóticas en el miocardio, válvulas miocárdicas y endocardio; e hígado decolorado y moteado junto con pequeños focos blancos y hemorragias petequiales o equimóticas. El análisis histopatológico mostró necrosis de hepatocitos y cuerpos de inclusión basófilos en los hígados. Se realizaron pruebas moleculares para la detección de adenovirus del pollo (FAdV), virus de la influenza aviar H5, virus de la enfermedad de Newcastle, virus de la bronquitis infecciosa aviar, virus de influenza tipo H9N2, virus de la anemia infecciosa de los pollos, virus de la enfermedad infecciosa de la bolsa, virus de la enfermedad de Marek, Ornithobacterium rhinotracheale, Mycoplasma gallisepticum y Mycoplasma synoviae con resultados positivos para adenovirus del pollo, virus de la anemia infecciosa, virus vacunal de Gumboro y virus de la bronquitis infecciosa serotipos 793B y variante I. El árbol filogenético basado en la asa 1 del gene del hexon demostró la presencia del serotipo 4 del adenovirus del pollo (FAdV-4) que era idéntico al aislamiento pakistaní PARC-1/98. Debido a que fue la primera detección de un adenovirus de pollo serotipo 4 en Irán, el programa de despoblación sanitario se aplicó de inmediato sobre la base de lesiones macroscópicas del síndrome de hepatitis-hidropericardio y por reacción de PCR positiva en el líquido pericárdico gelatinoso. Parece que esta estrategia de erradicación tuvo éxito porque no se notaron brotes durante dos meses después del brote inicial. Se concluyó que el uso de líneas base de patología macroscópica, el diagnóstico rápido de la enfermedad y la estrecha colaboración entre los sectores gubernamental y privado fueron los factores críticos que ayudaron a controlar localmente la primera presentación del síndrome de hepatitis-hidropericardio en Irán.Key words: hepatitis-hydropericardium syndrome, FAdV-4, control measures, Iran.

Effect of Wetting and Dewetting Dynamics on Atomic Force Microscopy Measurements.

Water bridge dynamics between an atomic force microscopy (AFM) tip and a flat substrate is studied by using a multibody dissipative particle dynamics (MDPD) model. First, the numerical model is validated by comparing the present results of droplet contact angles and liquid bridges with those reported in the literature. Then, the ability of MDPD to capture the meniscus shape and behavior for different operating conditions and geometric parameters is examined for both static and dynamic cases. Hence, several parametric studies and analyses of the AFM tip configuration and its operating conditions are reported. It is found that a critical capillary number of about 0.001 is calculated based on 5% change on the force measurements between the static and dynamic results. It is also demonstrated that the hysteresis behavior in the capillary force exerted on the AFM tip can be successfully predicted by using the MDPD model when the tip approaches or retracts from the substrate. Moreover, there is an excellent agreement in the results of breakup distance for different water bridge volumes between the predictions of the MDPD model and the theory. Also, the hysteresis of capillary force exerted on an AFM tip composed of multibody design is studied. The prediction on the transition of the capillary force vs distance between the AFM tip and the substrate is in good agreement with the experimental results. Therefore, we demonstrate a validated MDPD model which can successfully capture liquid bridge dynamics. This model can be used as a powerful design tool for meniscus manipulation technology, such as dip-pen nanolithography, as well as for studying dynamic, e.g., tapping mode AFM tip, interactions with a liquid bridge.

Clinical measures of obesity and cumulative cardiometabolic risk in adolescents.

Obesity tracks from childhood to adulthood most strongly of all cardiometabolic risk factors. To determine relationship of body mass index (BMI) and waist circumference (WC) with cardiometabolic risk (dyslipidemia, hyperglycemia and elevated blood pressure) in a large U.S. population ages 12-19 and demographic subgroups. Pooled 1999-2014 National Health and Nutrition Examination Survey data were analyzed (N = 23 438). In addition to standard cutoffs of BMI and WC, risk levels were identified for each laboratory variable: HDL-cholesterol, LDL-cholesterol, triglycerides, total cholesterol (category = lipids); fasting glucose, glycated haemoglobin (category = glucose); systolic/diastolic pressures (category =blood pressure). Within each category, being high-risk on any of the variables was high-risk; being borderline-risk on any, without being high-risk on any, was borderline-risk. Obesity severity was strongly associated with increased cardiometabolic risk, with prevalence of borderline-risk greater than high-risk. Anthropometric indicators in males and Hispanics, versus females and Whites/Blacks, respectively, had stronger associations with cardiometabolic risks. BMI and WC performed well for identifying adolescents with at least one borderline-risk or high-risk level measure for lipids, glucose and blood pressure; relationship strength varying by gender and race/ethnicity. Thus, to prevent or better manage clinical diseases of adolescents with elevated BMI and/or WC, all recommended laboratory tests are warranted.

Cardiac renin-angiotensin system (gene expression) and plasma angiotensin II in chickens with T3-induced pulmonary hypertension.

The relative expression of renin, angiotensin converting enzyme (ACE) and angiotensin II type 1 receptor (AT1R) was performed using quantitative real-time PCR on tissue from the heart ventricles to investigate the effect of 3,5,3'-l-triiodothyronine (T3)-induced pulmonary hypertension syndrome (PHS) in broiler chickens at 12 and 42 d of age. Plasma angiotensin II was also measured using enzyme immunoassay at 12, 21, 28, 32 and 42 d. Renin transcript in the right ventricle of the treated group was significantly increased at 12 d and decreased at 42 d of age compared to controls. ACE transcript in the left ventricle of the treated group was significantly increased at 42 d of age compared to controls. In the right ventricle, the expression of this gene was significantly increased at 12 d while decreased at 42 d of age in the treated group compared to control. AT1R transcript in the right ventricle of the treated group was significantly increased at 12 and 42 d of age compared to control. In the left ventricle of the treated group, the transcript of this gene was only higher at 12 d of age than control. Plasma angiotensin II was significantly increased in pulmonary hypertensive chickens at 28 d of age compared to control. It is concluded that gene expression of renin, ACE and AT1R was relatively upregulated in the heart of chickens developing pulmonary hypertension. The right ventricle of hearts from pulmonary hypertensive chickens showed considerable reductions of renin/ACE and elevation of AT1R which may provide evidence of diminished responsiveness of the renin-angiotensin system.

Muscle synergy analysis in transtibial amputee during ramp ascending activity.

In developed countries, the highest number of amputees are elderly with transtibial amputation. Walking on inclined surfaces is difficult for amputees due to loss of muscle volume and strength thereby transtibial amputees (TA) rely on the intact limb to maintain stability. The aim of this study was to use the concatenated non-negative matrix factorization (CNMF) technique to calculate muscle synergy components and compare the difference in muscle synergies and their associated activation profiles in the healthy and amputee groups during ramp ascending (RA) activity. Healthy subjects' dominant leg and amputee's intact leg (IL) were considered for recording surface electromyography (sEMG). The muscle synergies comparison showed a reasonable correlation between the healthy and amputee groups. This suggests the central nervous system (CNS) activates the same group of muscles synergistically. However, the activation coefficient profile (C) results indicated statistically significant difference (p <; 0.05) in some parts of the gait cycle (GC) in healthy and amputee groups. The difference exhibited in activation profiles of amputee's IL could be due to the instability of the prosthetic leg during the GC which resulted in alteration of the IL muscles activations. This information will be useful in rehabilitation and in the future development of prosthetic devices by using the IL muscles information to control the prostheses.

A histological study of pulp reaction to various water/powder ratios of white mineral trioxide aggregate as pulp-capping material in human teeth: a double-blinded, randomized controlled trial.

AIM: To compare the histological pulp reaction to various water/powder ratios of white mineral trioxide aggregate (MTA) as a pulp-capping material in healthy human teeth. METHODOLOGY: Twenty-nine disease-free maxillary and mandibular third molar teeth, scheduled for extraction, were exposed mechanically and then capped with 0.28, 0.33 and 0.40 water/powder ratios of white MTA (ProRoot; Dentsply Maillefer, Ballaigues, Switzerland) and restored with glass ionomer. After 30 days, the teeth were extracted, resected apically and immersed in 10% formalin. For histological processing, the teeth were sectioned buccolingually in 5-µm-thick slices, stained with Haematoxylin and Eosin and evaluated by a light microscope. Samples were evaluated for intensity and type of inflammation, presence of necrosis, as well as continuity, morphology and thickness of calcified bridges. The data were analysed by Kruskal-Wallis and Mann-Whitney tests. RESULTS: There were no significant differences in the diameter, morphology and continuity of the calcified bridges, intensity and type of inflammation or presence of necrosis (P > 0.05) in the pulps covered by MTA with various water/powder ratios. Two teeth failed to display a calcified bridge, and one had a pulp necrosis. CONCLUSION: Water-to-powder ratios of MTA had no significant influence on the histological outcome of direct pulp capping on healthy pulps.

An examination of five spatial disease clustering methodologies for the identification of childhood cancer clusters in Alberta, Canada.

Cluster detection is an important part of spatial epidemiology because it may help suggest potential factors associated with disease and thus, guide further investigation of the nature of diseases. Many different methods have been proposed to test for disease clusters. In this paper, we study five popular methods for detecting spatial clusters. These methods are Besag-Newell (BN), circular spatial scan statistic (CSS), flexible spatial scan statistic (FSS), Tango's maximized excess events test (MEET), and Bayesian disease mapping (BYM). We study these five different methods by analyzing a data set of malignant cancer diagnoses in children in the province of Alberta, Canada during 1983-2004. Our results show that the potential clusters are located in the south-central part of the province. Although, all methods performed very well to detect clusters, the BN and MEET methods identified local as well as general clusters.

Health attitude scale construction: importance of psychometric evidence.

OBJECTIVE: To identify 18 steps to 3-component attitude scale construction. METHODS: Examples are provided to illustrate each step. RESULTS: The presentation primarily focused on specific steps in constructing meaningful health attitude scales. Further, the importance and concept of various forms of realibilty and validity are provided. Special attention is given to factors affectiving psychometric evidence. CONCLUSIONS: In order to meaningfully measure health related attitudes, researchers and evaluators should consider following the steps outline in the presentation.

Health promotion beliefs and practices among pharmacists.

OBJECTIVE: To examine the health promotion beliefs and practices of pharmacists. DESIGN: Cross-sectional mail survey. SETTING: Indiana. PARTICIPANTS: Staff pharmacists. METHODS: Of the 1,440 registered Indiana pharmacies, one-half were selected using a systematic random process. Questionnaires were mailed to the 720 selected pharmacies asking one staff pharmacist to complete a 73-item questionnaire. RESULTS: A total of 552 pharmacists responded to the questionnaire, providing a 76.7% response rate. The majority of pharmacists believed that 10 of the 20 behaviors encouraged by national health objectives were very important for the average person. There was, however, considerably less agreement among pharmacists about the importance of the other 10 behaviors and practices. Pharmacists' involvement, preparation, and confidence in specific health promotion areas and activities were limited. A number of barriers were cited by respondents that could have hindered pharmacists' involvement in public health education activities. Despite these hindrances, pharmacists appear to be making strides toward providing health promotion education and activities. CONCLUSION: Pharmacists are readily accessible sources of information concerning the importance of needed lifestyle factors on health. They can provide valuable education to patients about improvement of lifestyles as a routine component of pharmaceutical care.

Evaluation of HIV/AIDS education in Russia using a video approach.

HIV/AIDS has intruded upon the geographic, political, ethnic, gender, and sexual orientation of communities all over the world. As of April 1999, Russia has recorded approximately 13,532 cases of HIV infection. Since the costs of treatment are expensive for many countries, and especially for Russia, educational intervention appears to offer the most effective and affordable solution. A quasi-experimental design, with pre/post tests and intervention (through video education)/control groups, was used to study 20 public schools in St. Petersburg, Russia. Results confirmed the lack of HIV/AIDS education in schools and insufficient information sources from parents, friends, and public health education. ANCOVA statistics demonstrated that use of video education significantly improved students' scores on knowledge and attitudes related to HIV/AIDS prevention. Thus, health educators should consider video education as an effective and efficient tool to present facts to a young audience when they face constraints of shortage of funds, lack of trained teachers, and scarcity of related information.

Pharmacists' concerns and suggestions related to the sale of tobacco and alcohol in pharmacies.

A random sample of Pharmacists in Indiana was selected and surveyed regarding their thoughts and comments on the sale of tobacco and alcoholic products by pharmacies. A total of 212 pharmacists provided written responses to an open-ended question. The replies were then synthesized into major themes. The majority of comments categorized revealed strong opposition to pharmacies selling tobacco and alcohol products. The most frequently occurring message regarding the sale of these products by pharmacies was that it goes against the pharmacist's role as a professional health care provider and may harm their image. For some pharmacists, this issue posed a moral dilemma between caring for patient safety and the economic incentives provided by the sale of tobacco and alcohol. Based on these findings, a follow-up phone survey was conducted to solicit suggestions from pharmacists on how to advocate against the sale of tobacco and alcohol by pharmacies. The majority of pharmacists strongly recommended that they need to do more among themselves and within their store environment, rather than have regulations imposed on pharmacies when it comes to limiting the sale of these products.

Pharmacists' opinions and practices related to the sale of cigarettes and alcohol--a follow-up study.

Opinions that pharmacists hold and their practices concerning sale of cigarettes and alcohol are of interest to health experts. As a follow-up to a 1990 statewide survey of pharmacists opinions and practices related to the sale of cigarettes and alcohol, this study was designed (1) to determine current opinions and practices of pharmacists' related to the sale of cigarettes and alcohol; (2) compare these findings with results from the 1990 study; and (3) to gather new information on pharmacists' practice of health promotion activities. A structured survey questionnaire was designed and reviewed by a jury of experts and subsequently administered to half of the 1340 pharmacies in Indiana. Collected data were analyzed by using descriptive and inferential statistical methods. Findings reveal that 64 percent of responding pharmacists sell cigarettes in their stores even though 82 percent think that their stores should not sell cigarettes. Approximately 42 percent of the pharmacies sell alcoholic beverages while more than two-thirds of the pharmacists (68%) think pharmacies should not sell alcoholic beverages. These findings represent a decline of 7.2 percent in pharmacies that sell cigarettes and a 6.8 percent increase in pharmacies selling alcoholic beverages compared to the 1990 study. Study results also revealed that most pharmacists agree the use of cigarettes and alcohol were important causes of morbidity and mortality and that pharmacists should play a role in health promotion and disease prevention to the public. However, the majority do not ask their patients about their smoking and alcohol habits and do not participate in health education/promotion programs for the general community.

Trends of public opinion on tobacco use and public policy.

This study examined the trends and stability of public opinion related to tobacco use and raising tobacco taxes in Indiana. Using a structured questionnaire, a sample of 800 households was randomly selected, and the adults were interviewed by telephone in 1989, 1990 and 1992 by the Indiana University Center for Survey Research. The data were subjected to descriptive and chi-square statistical procedures. The results of the study indicate that more than 80% agreed that secondhand smoke is a health threat, and the opinions remained practically stable for the duration of the study. Most respondents agreed that public places should be required to have nonsmoking areas. Further, most people favored a tax increase on cigarettes, and there was an upward trend toward more people favoring a tax increase in recent years. It was concluded that the tax on tobacco should be increased for public health reasons.

Prevalence of adolescent health risk behaviors: school health implications.

This state survey randomly selected 2,037 ninth- and twelfth-grade students to determine the onset and prevalence of health risk behaviors. A higher pattern of risk-taking behavior was found among younger in comparison with older students. Gender differences were also noted. Implications for school health intervention are discussed.

Cross-cultural study of samples of adolescents' attitudes, knowledge, and behaviors related to smoking.

This study examined the smoking behavior, knowledge, and attitudes of 413 students in Grades 7 and 8 in the United States and 393 such students in the People's Republic of China. A survey questionnaire developed by Pederson and Lefcoe was adapted and then translated into Mandarin. There were statistically significant differences between the students of the two countries in knowledge, attitude, and behavior related to smoking. Families played an important role in relation to smoking among this age group.

Public opinion on tobacco use, its taxes and public policy.

Public opinion is an important vehicle in shaping legislative efforts and public policy related to tobacco use and related taxes. This study examined public opinion about policy related to tobacco use and raising tobacco taxes in a Midwestern state. The American Lung Association of Indiana provided a grant to sponsor this project. A structured questionnaire was developed, field tested and revised for the final data collection procedure. A sample of 800 adult subjects was randomly selected and telephone interviewed by the Indiana University Center for Survey Research. The collected data were subjected to descriptive and inferential statistics. The data revealed a good representative sample of adult males and females for various age groups and socioeconomic backgrounds. Among other findings, 29% were currently smokers, 23% were former smokers, and 47.5% never smoked. More than 90% support a requirement that public places have nonsmoking areas. About 85% favor an increase in cigarette taxes to pay for health education and tobacco-related research. The relationship of the subjects' gender, education and income to their opinion about the various aspects of tobacco, taxes and related policies were examined.

The 1993 ASHA membership survey: members speak up.

Professional organizations, to better serve their members, must strive to communicate regularly with the membership at large and to learn about members' needs, interests, and personal and professional status. The membership survey of the American School Health Association was sent to a stratified random sample of 500 members. A 68% response rate was achieved after a presurvey announcement and two follow-up mailings. Collected data was subjected to descriptive analysis and the chi-square test of significance. Survey findings indicated most ASHA members are satisfied with the overall operation and services of the Association. Data revealed satisfaction with overall quality, format, content, and appearance of the Journal of School Health. The ASHA national school health conference, The PULSE, and other ASHA publications also received strong approval. One-half the respondents reported they are required to earn continuing education credit. More than two-thirds of respondents had never attended a national conference. Overall ratings of various ASHA functions were statistically related to age, gender, income level, educational background, and primary discipline.

Cigarette smoking as a predictor of alcohol and other drug use by children and adolescents: evidence of the "gateway drug effect".

Data from a statewide survey, conducted by the Indiana Prevention Resource Center, of 20,629 Indiana students in grades 5-12 were analyzed to determine the extent to which cigarette smoking predicted use of alcohol and other drugs and acted as a so-called "gateway drug." A three-stage purposive/quota cluster sampling strategy yielded a representative sample of Indiana students, stratified by grade. Cross-tabulated data revealed a strong, dose-dependent relationship between smoking behavior and binge drinking, as well as use of alcohol and illicit drugs. Daily pack-a-day smokers were three times more likely to drink alcohol, seven times more likely to use smokeless tobacco, and 10-30 times more likely to use illicit drugs than nonsmokers. A stepwise multiple regression analyzed the role that the student's perceptions of the risk of using drugs and of peer approval/disapproval of the student's drug use, gender, grade in school, and ethnic background played in predicting alcohol and other drug use.

HIV/AIDS education in Indiana public schools, grades 7-12.

Indiana secondary school principals were surveyed regarding HIV/AIDS education status in their schools. A questionnaire from the Centers for Disease Control was evaluated then modified for the survey. More than 520 school principals responded to the questionnaire mailed to all 680 school principals, grades 7-12, in Indiana. Most schools offer some form of HIV/AIDS education in grades 7-12. HIV education was taught by teachers with different educational backgrounds and school roles. Almost 60% of school principals believe students are at risk for HIV infection. Tests of statistical significance were conducted with regard to principals' opinions and actual practice of HIV/AIDS education in schools. Most schools have responded to the need for HIV/AIDS education, but with a variety of formats, teaching personnel, and teacher training.