RESUMO
The influence of humoral and cell-mediated immunity on the formation of resistance in mice immunized with inactivated Lassa virus was evaluated. As a result of immunization, both nonspecific and specific immune response developed, the main rule in the formation of resistance belonging to specific immunity.
Assuntos
Arenaviridae/imunologia , Imunização , Vírus Lassa/imunologia , Vacinas Virais/imunologia , Animais , Anticorpos Antivirais/análise , Citotoxicidade Imunológica , Imunidade Inata , Imunização Passiva , Interferons/sangue , Células Matadoras Naturais/imunologia , Ativação Linfocitária , Camundongos , Camundongos Endogâmicos CBA , Testes de Neutralização , Fatores de Tempo , Vacinas de Produtos Inativados/imunologiaAssuntos
Anticorpos Antivirais/biossíntese , Antígenos Virais/imunologia , Arenaviridae/imunologia , Febre Lassa/imunologia , Vírus Lassa/imunologia , Animais , Encéfalo/microbiologia , Hemaglutinação por Vírus , Técnicas Imunoenzimáticas , Imunoglobulina M/análise , Rim/microbiologia , Febre Lassa/microbiologia , Fígado/microbiologia , Camundongos , Baço/microbiologiaRESUMO
Pathogenicity for guinea pigs and white mice of various Lassa virus variants: native, having had 1 passage in Vero cell culture and 4 passages in newborn white mouse brain; a virus having gone through 10 passages in Vero cells and 8 mouse brain passages (variant No. 10); a small-plaque clone derived from variant No. 10 by the method of plaque-to-plaque cloning (variant No. 11k), was studied. Both the native virus and variant No. 10 were found to be similarly pathogenic for susceptible laboratory animals, while the small-plaque variant of Lassa virus became non-fatal for guinea pigs and white mice. The decline of pathogenic properties in variant No. 11k was shown not to be associated with the presence of temperature-sensitive mutants or defective interfering particles.