Topical formulations containing Copaifera duckei Dwyer oleoresin improve cutaneous wound healing.

OBJECTIVE: Evaluation of the healing and toxicological effects of Copaifera duckei Dwyer oleoresin (CDO). MATERIALS AND METHODS: Rodents with skin lesions were divided into nine groups, including daily treatments with 1, 3 and 10% CDO, collagenase, antibiotic ointment and control groups, for 14 days. RESULTS: Treatment with 10% CDO reduced skin edema and hyperplasia, demonstrating anti-inflammatory effect of the oil. Reduction in the wound area was observed, indicating the healing effect of CDO. Histopathological analysis showed increases in angiogenesis and re-epithelialization in animals treated with the highest concentration. On the other hand, no alterations in ulcerations, inflammatory infiltrate, hemorrhage, congestion, degeneration, percentage of collagen fibers, number of cells stained with anti-macrophage migration inhibitory factor, or density of area stained with anti-collagen I and III were found. Toxicogenetic analysis revealed no differences in micronucleus frequencies or in the ratio of polychromatic erythrocytes to total erythrocytes between treated and negative control, demonstrating the absence of genotoxicity and cytotoxicity, respectively. There was no difference in levels of liver enzymes among groups, indicating the absence of hepatotoxicity. CONCLUSION: Formulations of CDO exerted beneficial effects on the stages of cutaneous wound healing and are promising options for the treatment of wounds.

Use of an allogenous tunica vaginalis conserved in 98% glycerine for keratoplasty in dogs

We evaluated the use of an allogenous tunica vaginalis conserved in 98% glycerine for lamellar keratoplasty in dogs. Sevendogs were submitted to keratoplasty and clinically evaluated during the initial (1 to 15 days), intermediate (16 to 30 days) and late(31 to 120 days) postoperative periods. The corneas were evaluated by light microscopy at 1, 3, 7, 15, 30, 60 and 120 days aftersurgery. Photophobia, epiphora, blepharospasm, corneal edema, ocular secretion and chemosis were observed during theinitial periods, and tended to disappear during the intermediate periods. Vascular neoformation started around the 4th day, witha maximum intensity on the 25th day and a gradual regression thereafter, and tended to disappear between the 60th and 120thday. The graft itself and adjacent areas showed a tendency towards the reestablishment of transparency during the latepostoperative periods. With respect to morphology, an inflammatory infiltrate characterized by the invasion of polymorphonuclearand mononuclear leukocytes was observed which disappeared by the 60th postoperative day, when the graft was found to beincorporated and the stroma returned to normal. The present results led us to conclude that the allogenous tunica vaginalisconserved in 98% glycerine was feasible and efficient for lamellar keratoplasty in dogs.