Multifocal Analysis of Acute Pain After Third Molar Removal.

Background: To analyze the pain modulation capacity profile in a Brazilian population, the relationship between opioid receptor (OPRM1) and Catechol-O-methyltransferase (COMT) 1polymorphisms and pain modulation capacity was determined through preoperative pain modulation tests and acute postoperative pain control evaluation, swelling, and trismus in 200 volunteers undergoing lower third molar removal. Methods: Psychologic and clinical parameters were measured. Patient DNA was sequenced for single nucleotide polymorphisms in OPRM1 and COMT, and the salivary concentration of interleukin (IL)-2 (IL)-6, interferon (IFN)-γ and tumor necrosis factor (TNF)-α was evaluated. Primary outcomes were the influence of all predictors on the fluctuation of pain intensity using a visual analogue scale (VAS), and swelling and trismus on the 2nd and 7th postoperative days. Preoperative pain modulation capacity (CPM), pain catastrophizing scale (PCS), body mass index (BMI), and surgery duration and difficulty were evaluated. Results: Salivary concentration of IFN-γ and IL-2 as well as the duration of surgery influenced the fluctuation of postoperative pain in the VAS, and in the sum of the differences in pain intensity test at 8, 48, and 96 h. BMI influenced swelling, while both BMI and COMT haplotype influenced trismus on the 2nd postoperative day. Conclusion: Polymorphisms in COMT, salivary concentrations of IL-2 and IFN-γ, BMI, and duration of surgery were predictors for pain fluctuation, swelling, and trismus on the 2nd day after lower third molar extraction. This therapy was effective in controlling inflammatory symptomatology after lower third molar extraction and ibuprofen was well tolerated by patients. Clinical Trial Registration: www.ClinicalTrials.gov, identifier NCT03169127.

Forensic parameters and allele frequency distributions of 16 STR in a Paraguayan population.

Paraguay is a Latin American country located in the central zone of South America. There is no official data on the ethnic composition of its population. The European colonization and the wars through which the country crossed were fundamental for its current genetic composition. Here, we analyzed sixteen short tandem repeats (STRs) used in the CODIS system in 259 Paraguayan individuals.

Efficacy of Ketoprofen With or Without Omeprazole for Pain And Inflammation Control After Third Molar Removal.

In view of the gastrointestinal problems generated by the ketoprofen use, the ketoprofen association with omeprazole is available on the market. However, this association efficacy in acute pain control has not been established. Bilateral extraction of lower third molars in similar positions is currently the most used model for the evaluation and investigation of the efficacy and pharmacological effects of new compounds for the treatment of acute postoperative pain. The randomized and crossover study consisted in evaluating the clinical efficacy of therapy performed by ketoprofen 100 mg (twice daily-b.i.d.) versus ketoprofen 200 mg + omeprazole 20 mg (once daily-q.d.) to pain, swelling and trismus control in the bilateral extraction model of lower third molars in similar positions in two different appointments, in 50 volunteers. Volunteers reported significantly less postoperative pain at various post-operative periods and consumed less rescue analgesic medication (acetaminophen 750 mg) throughout the study when they took the combination of ketoprofen 200 mg + omeprazole 20 mg (q.d.). Following administration of both study drugs, no gastrointestinal adverse reactions were reported by volunteers. Furthermore, the evaluations of the drugs in pain control by the volunteers were significantly favorable to ketoprofen 200 mg + omeprazole 20 mg (q.d.). For swelling and trismus control, the treatments presented similar results. In conclusion, when volunteers took ketoprofen 200 mg + omeprazole 20 mg (q.d.), they reported significantly less postoperative pain at various post-surgical periods and consumed less rescue analgesic medication throughout the study compared with ketoprofen 100 mg (b.i.d).

Periodontal status and pathogenic bacteria after gastric bypass: a cohort study.

AIM: The aim this study was to evaluate the influence of gastric bypass surgery (GBS) on periodontal disease and quantify the periodontopathogenic bacteria in patients undergoing this surgery. MATERIAL AND METHODS: This prospective study was composed of 50 patients who underwent bariatric surgery and the data collection was performed in three periods pre-operative, 6 (6M) and 12 months (12 M) postoperative. The oral clinical examination to assess periodontal disease; gingival fluid sample collection for quantification of the periodontopathogenic bacteria Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, and Prevotella intermedia using q-PCR; body mass index (BMI) and for collection of the individual's health-related data from medical files. RESULTS: There was a significant reduction in serum C-reactive protein (CRP) and glucose levels after surgery. The mean probing pocket depth (PPD) and clinical attachment level (CAL) increased significantly in the postoperative period of 6 months (p = 0.001). In the same period, the amount of P. gingivalis increased (p = 0.028) and the other bacteria decreased slightly (p > 0.050). In the presence of P. gingivalis, T. forsythia, T. denticola and P. intermedia, a poor periodontal condition was observed. CONCLUSION: The periodontal disease increased in severity and P. gingivalis increased after GBS. A systemic inflammation resolution due to bariatric surgery in obese subjects does not seem to affect the course of periodontal disease.

Estudo de associação entre antígenos leucocitários humanos e doença de Jorge Lobo / Study of the association between human leukocyte antigens and Jorge Lobo's disease

A doença de Jorge Lobo é uma micose cutânea/subcutânea de evolução crônica, causada pelo fungo Lacazia loboi. Devido às características epidemiológicas e poucos estudos relacionados aos aspectos imunológicos dessa doença, o objetivo do trabalho foi investigar uma possível associação das especificidades HLA de classe II em 21 pacientes portadores da doença de Jorge Lobo, comparando com indivíduos sadios de mesma etnia. As tipificações HLA foram realizadas pelo método de PCR-SSP. O resultado não revelou qualquer tipo de associação entre os antígenos HLA e doença de Jorge Lobo. Embora sem significância estatística, foi observada a diminuição da freqüência do antígeno HLA-DR7 no grupo dos pacientes em relação aos controles (0 por cento x 18 por cento), sugerindo uma associação negativa (protetora) entre HLA-DR7 e doença de Jorge Lobo. Contudo, estudos devem ser continuados, objetivando melhor entendimento nos mecanismos envolvidos na suscetibilidade e/ou proteção dessa doença.

[Study of the association between human leukocyte antigens and Jorge Lobo's disease]. / Estudo de associação entre antígenos leucocitários humanos e doença de Jorge Lobo.

Jorge Lobo's disease is a cutaneous/subcutaneous mycosis with a chronic course, caused by the fungus Lacazia loboi. Considering its epidemiology and the few studies on immunological aspects of this disease, the objective of the present study was to investigate a possible association between HLA class II specificities in 21 Jorge Lobo's disease patients, by comparing them with healthy individual of the same ethnic group. HLA typing was performed using PCR-SSP method. The result did not show any association between HLA antigens and Jorge Lobo's disease. Although not statistically significant, a decreased frequency of HLA-DR7 was observed in the patients compared to the controls (0% x 18%). This suggests a negative association (protective) between HLA-DR7 and Jorge Lobo's disease. Further studies, however, are needed for a better understanding of the susceptibility and/or protection mechanisms involved.

Complexo HLA e glaucoma crônico simples / HLA complex and chronic simplex glaucoma

Antígenos HLA-A e B foram determinados em 23 pacientes caucasóides portadores de glaucoma crônico simples. As freqüências antigênicas observadas foram comparadas às obtidas em amostras de populaçäo caucasóide de Säo Paulo. Os resultados obtidos näo revelaram associaçäo com nenhum antígeno HLA-A ou B isoladamente, porém sugeriram associaçäo com os haplotipos HLA-A9B12, A2B40 e A1B8. Estudo de distribuiçäo de haplotipos HLA entre irmäos portadores de glaucoma crônica simples ou de somente pressäo intraocular elevada revelou excesso de haplotipos em comum, sugerindo a presença no complexo HLA, ou próximo ao mesmo, de genes de susceptibilidade a glaucoma crônico simples