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1.
Neuroscience ; 254: 45-60, 2013 Dec 19.
Artigo em Inglês | MEDLINE | ID: mdl-24045101

RESUMO

Chemical communication is widely used among various organisms to obtain essential information from their environment required for life. Although a large variety of molecules have been shown to act as chemical cues, the molecular and neural basis underlying the behaviors elicited by these molecules has been revealed for only a limited number of molecules. Here, we review the current knowledge regarding the signaling molecules whose flow from receptor to specific behavior has been characterized. Discussing the molecules utilized by mice, insects, and the worm, we focus on how each organism has optimized its reception system to suit its living style. We also highlight how the production of these signaling molecules is regulated, an area in which considerable progress has been recently made.


Assuntos
Células Quimiorreceptoras/fisiologia , Nervo Olfatório/fisiologia , Condutos Olfatórios/fisiologia , Transdução de Sinais/fisiologia , Olfato/fisiologia , Animais , Humanos , Órgão Vomeronasal/fisiologia
2.
Results Probl Cell Differ ; 47: 121-38, 2009.
Artigo em Inglês | MEDLINE | ID: mdl-19083129

RESUMO

The insect olfactory system is a suitable model for exploring molecular function of odorant receptors, axonal projection of olfactory receptor neurons onto secondary neurons, and the neural circuit for odor perception. Recent progress in the study of insect olfaction revealed that the heteromeric insect olfactory receptor complex forms a cation nonselective ion channel directly gated by odor or pheromone ligands independent of known G-protein signaling pathways. Despite fundamental differences in transduction machineries between insects and vertebrates, the anatomical and functional features of insect odor-coding strategy are similar and thus justify any consideration of mammalian olfaction in the study of insects. The understanding of the molecular mechanism of insect olfaction will help in the development of insect repellents for controlling insect pest and vector populations for a wide range of pathogens.


Assuntos
Insetos/fisiologia , Receptores Odorantes/fisiologia , Transdução de Sinais/fisiologia , Olfato/fisiologia , Animais , Comportamento Animal/fisiologia , Perfilação da Expressão Gênica , Insetos/genética , Modelos Biológicos , Condutos Olfatórios/fisiologia , Receptores Odorantes/genética , Transdução de Sinais/genética , Olfato/genética
3.
J Neurosci ; 21(16): 6018-25, 2001 Aug 15.
Artigo em Inglês | MEDLINE | ID: mdl-11487625

RESUMO

The vertebrate olfactory system discriminates a wide variety of odorants by relaying coded information from olfactory sensory neurons in the olfactory epithelium to olfactory cortical areas of the brain. Recent studies have shown that the first step in odor discrimination is mediated by approximately 1000 distinct olfactory receptors, which comprise the largest family of G-protein-coupled receptors. In the present study, we used Ca(2+) imaging and single-cell reverse transcription-PCR techniques to identify mouse olfactory neurons responding to an odorant and subsequently to clone a receptor gene from the responsive cell. The functionally cloned receptors were expressed in heterologous systems, demonstrating that structurally related olfactory receptors recognized overlapping sets of odorants with distinct affinities and specificities. Our results provide direct evidence for the existence of a receptor code in which the identities of different odorants are specified by distinct combinations of odorant receptors that possess unique molecular receptive ranges. We further demonstrate that the receptor code for an odorant changes with odorant concentration. Finally, we show that odorant receptors in human embryonic kidney 293 cells couple to stimulatory G-proteins such as Galphaolf, resulting in odorant-dependent increases in cAMP. Odor discrimination is thus determined by differences in the receptive ranges of the odorant receptors that together encode specific odorant molecules.


Assuntos
Olfato/fisiologia , Animais , Cálcio/metabolismo , Células Cultivadas , Clonagem Molecular , AMP Cíclico/metabolismo , Corantes Fluorescentes , Subunidades alfa Gs de Proteínas de Ligação ao GTP/metabolismo , Expressão Gênica , Humanos , Rim/citologia , Rim/metabolismo , Camundongos , Camundongos Endogâmicos BALB C , Dados de Sequência Molecular , Odorantes , Neurônios Receptores Olfatórios/química , Neurônios Receptores Olfatórios/citologia , Neurônios Receptores Olfatórios/metabolismo , Receptores Odorantes/genética , Receptores Odorantes/metabolismo , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Homologia de Sequência de Aminoácidos , Transdução de Sinais/fisiologia , Relação Estrutura-Atividade , Especificidade por Substrato/fisiologia , Transfecção
4.
Life Sci ; 68(19-20): 2199-206, 2001 Apr 06.
Artigo em Inglês | MEDLINE | ID: mdl-11358328

RESUMO

The olfactory systems of vertebrates have a remarkable capacity to recognize and discriminate thousands of different odorant molecules. The initial step in the process of odorant perception is the recognition of volatile odorant molecules by a group of roughly one thousand G protein-coupled odorant receptors that are expressed on the surface of olfactory neuronal cilia. The aims of this study were to obtain functional evidence that these putative odorant receptors recognize and respond to specific odorant molecules, and to elucidate the mechanisms of odorant discrimination in vertebrate olfaction at a receptor level. In order to identify odorant receptors that specifically recognize a particular odorant of interest, we developed a functional cloning strategy in an odorant-directed manner by combining Ca2+-recording and single cell RT-PCR techniques. We then adopted an adenovirus-mediated expression system or a chimeric receptor approach to reconstitute the functionally cloned receptors for further biochemical analyses. We herein describe how we obtained experimental evidence for a combinatory mechanism of odorant recognition by examining the diversity of odorant receptors that recognize a particular odorant of interest, and by determining ligand specificity and structure-function relationships for individual odorant receptors.


Assuntos
Clonagem Molecular , Odorantes , Neurônios Receptores Olfatórios/fisiologia , Receptores Odorantes/fisiologia , Adenoviridae , Animais , Cálcio/metabolismo , Vetores Genéticos , Camundongos , Camundongos Endogâmicos BALB C , Modelos Biológicos , Bulbo Olfatório/fisiologia , Neurônios Receptores Olfatórios/citologia , RNA/análise , Receptores Odorantes/biossíntese , Receptores Odorantes/genética , Proteínas Recombinantes de Fusão , Reação em Cadeia da Polimerase Via Transcriptase Reversa
5.
Biochemistry ; 38(42): 13787-94, 1999 Oct 19.
Artigo em Inglês | MEDLINE | ID: mdl-10529223

RESUMO

The pleckstrin homology (PH) domain, identified in numerous signaling proteins including the beta-adrenergic receptor kinase (betaARK), was found to bind to various phospholipids as well as the beta subunit of heterotrimeric G proteins (Gbeta) [Touhara, K., et al. (1994) J. Biol. Chem. 269, 10217-10220]. Several PH domain-containing proteins are also substrates of protein kinase C (PKC). Because RACK1, an anchoring protein for activated PKC, is homologous to Gbeta (both contain seven repeats of the WD-40 motif), we determined (i) whether a direct interaction between various PH domains and RACK1 occurs and (ii) the effect of PKC on this interaction. We found that recombinant PH domains of several proteins exhibited differential binding to RACK1. Activated PKC and the PH domain of beta-spectrin or dynamin-1 concomitantly bound to RACK1. Although PH domains bind acidic phospholipids, the interaction between various PH domains and RACK1 was not dependent on the phospholipid activators of PKC, phosphatidylserine and 1, 2-diacylglycerol. Binding of these PH domains to RACK1 was also not affected by either inositol 1,4,5-triphosphate (IP(3)) or phosphatidylinositol 4,5-bisphosphate (PIP(2)). Our in vitro data suggest that RACK1 binds selective PH domains, and that PKC regulates this interaction. We propose that, in vivo, RACK1 may colocalize the kinase with its PH domain-containing substrates.


Assuntos
Proteínas Sanguíneas/metabolismo , Fragmentos de Peptídeos/metabolismo , Peptídeos/metabolismo , Fosfoproteínas , Receptores de Superfície Celular/metabolismo , Animais , Sítios de Ligação , Plaquetas/química , Proteínas Sanguíneas/química , Dinamina I , Dinaminas , Ativação Enzimática , GTP Fosfo-Hidrolases/química , GTP Fosfo-Hidrolases/metabolismo , Humanos , Isoenzimas/química , Isoenzimas/metabolismo , Modelos Químicos , Fragmentos de Peptídeos/química , Peptídeos/química , Fosfolipídeos/química , Proteína Quinase C/química , Proteína Quinase C/metabolismo , Proteína Quinase C beta , Estrutura Terciária de Proteína , Ratos , Receptores de Quinase C Ativada , Receptores de Superfície Celular/química , Homologia de Sequência de Aminoácidos
6.
Biochem Biophys Res Commun ; 255(1): 169-74, 1999 Feb 05.
Artigo em Inglês | MEDLINE | ID: mdl-10082674

RESUMO

Myosin II was identified as a binding protein to the pleckstrin homology (PH) domain of protein kinase B (PKB) in CHO cell extract by using the glutathione S-transferase-fusion protein as a probe. When myosin II purified from rabbit skeletal muscle was employed, myosin II was shown to bind almost exclusively to the PH domain of PKB among the PH domain fusion proteins examined. The purified myosin II bound to the PH domain of PKB with a Kd value of 1.1 x 10(-7) M. Studies with a series of truncated molecules indicated that the whole structure of the PH domain is required for the binding of myosin II, and the binding to the PH domain was inhibited by phosphatidylinositol 4,5-bisphosphate. These results suggest that myosin II is a specific binding protein to the PH domain of particular proteins including PKB.


Assuntos
Miosinas/metabolismo , Fosfoproteínas , Proteínas Serina-Treonina Quinases , Proteínas Proto-Oncogênicas/metabolismo , Animais , Sítios de Ligação/genética , Proteínas Sanguíneas/genética , Células CHO , Cricetinae , Ligação Proteica , Proteínas Proto-Oncogênicas/genética , Proteínas Proto-Oncogênicas c-akt , Coelhos
7.
Proc Natl Acad Sci U S A ; 96(7): 4040-5, 1999 Mar 30.
Artigo em Inglês | MEDLINE | ID: mdl-10097159

RESUMO

The olfactory system is remarkable in its capacity to discriminate a wide range of odorants through a series of transduction events initiated in olfactory receptor neurons. Each olfactory neuron is expected to express only a single odorant receptor gene that belongs to the G protein coupled receptor family. The ligand-receptor interaction, however, has not been clearly characterized. This study demonstrates the functional identification of olfactory receptor(s) for specific odorant(s) from single olfactory neurons by a combination of Ca2+-imaging and reverse transcription-coupled PCR analysis. First, a candidate odorant receptor was cloned from a single tissue-printed olfactory neuron that displayed odorant-induced Ca2+ increase. Next, recombinant adenovirus-mediated expression of the isolated receptor gene was established in the olfactory epithelium by using green fluorescent protein as a marker. The infected neurons elicited external Ca2+ entry when exposed to the odorant that originally was used to identify the receptor gene. Experiments performed to determine ligand specificity revealed that the odorant receptor recognized specific structural motifs within odorant molecules. The odorant receptor-mediated signal transduction appears to be reconstituted by this two-step approach: the receptor screening for given odorant(s) from single neurons and the functional expression of the receptor via recombinant adenovirus. The present approach should enable us to examine not only ligand specificity of an odorant receptor but also receptor specificity and diversity for a particular odorant of interest.


Assuntos
Odorantes , Neurônios Receptores Olfatórios/fisiologia , Receptores Odorantes/fisiologia , Adenoviridae , Animais , Cálcio/metabolismo , Clonagem Molecular , Primers do DNA , Fura-2 , Proteínas de Ligação ao GTP/fisiologia , Vetores Genéticos , Proteínas de Fluorescência Verde , Proteínas Luminescentes/genética , Camundongos , Camundongos Endogâmicos BALB C , Mucosa Olfatória/citologia , Neurônios Receptores Olfatórios/citologia , Receptores Odorantes/biossíntese , Receptores Odorantes/genética , Proteínas Recombinantes de Fusão/biossíntese , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Transcrição Gênica , Transfecção
8.
Biochem Biophys Res Commun ; 252(3): 669-74, 1998 Nov 27.
Artigo em Inglês | MEDLINE | ID: mdl-9837764

RESUMO

beta-Adrenergic receptor kinase (betaARK) plays a pivotal role in phosphorylating and desensitizing G protein coupled receptors by virtue of pleckstrin homology (PH) domain-mediated membrane translocation. betaARK is localized to the specific membrane compartment by betagamma subunits of G proteins (Gbetagamma) and phosphatidylinositol phosphates that specifically and coordinately bind to the carboxyl and amino terminus half, respectively, of the betaARK PH domain. To determine the function of the betaARK PH domain in intact cells, various point mutations were incorporated in the betaARK PH domain and the constructs were tested for their ability to agonist-dependently phosphorylate the muscarinic acetylcholine receptor or alpha-adrenergic receptor in COS-7 cells. It was found that selected mutations (i.e., W643A, L647A, and an Ala-insertion following Trp643) completely abolished betaARK's ability to phosphorylate the receptors in whole-cell labeling experiments. These residues are located in the carboxyl-terminal alpha-helix of the PH domain that is essential for binding to Gbetagamma. This site-directed mutation study provides molecular information on the mechanism and significance of the betaARK PH domain function in the intact cell system.


Assuntos
Proteínas Quinases Dependentes de AMP Cíclico/genética , Mutação , Fosfoproteínas , Homologia de Sequência de Aminoácidos , Sequência de Aminoácidos , Animais , Sítios de Ligação/genética , Proteínas Sanguíneas/química , Células COS , Linhagem Celular , Proteínas Quinases Dependentes de AMP Cíclico/química , Proteínas de Ligação ao GTP/metabolismo , Ligantes , Dados de Sequência Molecular , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosforilação , Rodopsina/metabolismo , Spodoptera , Quinases de Receptores Adrenérgicos beta
9.
FEBS Lett ; 417(2): 243-8, 1997 Nov 10.
Artigo em Inglês | MEDLINE | ID: mdl-9395305

RESUMO

Pleckstrin homology (PH) domains are discrete structural modules present in numerous proteins involved in signal transduction processes. In the case of the beta-adrenergic receptor kinase (betaARK), PH domain-mediated binding of two ligands, the betagamma subunits of heterotrimeric G proteins (Gbetagamma) and phosphatidylinositol 4,5-bisphosphate (PIP2), has been shown to be required for the kinase function. In this study, the ability of Gbetagamma and PIP2 to affect membrane localization of betaARK is used to define the ligand binding characteristics of the betaARK PH domain. The binding of these ligands to the PH domain of the intact kinase is shown to be cooperative, Gbetagamma increasing the affinity of the PH domain for PIP2. Notably, although PIP2-dependent membrane association of betaARK is observed at high concentrations of this lipid, in the absence of Gbetagamma, no receptor phosphorylation is observed. Peptides derived from the receptor intracellular loop inhibit the receptor phosphorylation without affecting the membrane translocation of the kinase complex, suggesting that betaARK activity does not necessarily correlate with the amount of betaARK associated with the membrane. These results point to a distinct role for each PH domain ligand in betaARK-mediated receptor phosphorylation. Strikingly, the ligand binding characteristics of the isolated betaARK PH domain fused to glutathione S-transferase are very different from those of the PH domain of the intact kinase. Thus, in contrast to the native protein, the isolated PH domain binds Gbetagamma and PIP2 independently and with no apparent cooperativity. That protein environment plays an important role in determining the ligand binding characteristics of a particular PH domain highlights the potential risks of inferring mechanisms from studies of isolated PH domains.


Assuntos
Proteínas Quinases Dependentes de AMP Cíclico/química , Proteínas de Ligação ao GTP/química , Fosfoproteínas , Animais , Sítios de Ligação , Proteínas Sanguíneas/química , Bovinos , Compartimento Celular , Membrana Celular/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Ligantes , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosforilação , Receptores Adrenérgicos beta/metabolismo , Proteínas Recombinantes , Transdução de Sinais , Relação Estrutura-Atividade , Quinases de Receptores Adrenérgicos beta
10.
Endocrinology ; 138(8): 3103-11, 1997 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-9231756

RESUMO

In this study, prostaglandin (PG) F2alpha was found to activate mitogen-activated protein (MAP) kinase and MAP kinase kinase (MEK) in cultured rat puerperal uterine myometrial cells. PGF2alpha stimulation also led to an increase in phosphorylation of raf-1, son of sevenless (SOS), and Shc. Furthermore, we examined the mechanism by which PGF2alpha induced MAP kinase phosphorylation. Both pertussis toxin (10 ng/ml), which inactivates Gi/Go proteins, and expression of a peptide derived from the carboxyl terminus of the beta-adrenergic receptor kinase 1 (betaARK1), which specifically blocks signaling mediated by the betagamma subunits of G proteins, blocked the PGF2alpha-induced activation of MAP kinase. Ritodrine (1 microM), which is known to relax uterine muscle contraction, attenuated PGF2alpha-induced tyrosine phosphorylation of MAP kinase. Moreover, to examine the role of MAP kinase pathway in uterine contraction, an inhibitor of MEK activity, PD098059, was used. Although MEK inhibitor had no effect on PGF2alpha-induced calcium mobilization, this inhibitor partially inhibited PGF2alpha-induced uterine contraction. These results provide evidence that PGF2alpha stimulates the MAP kinase signaling pathway in cultured rat puerperal uterine myometrial cells through Gbetagamma protein, suggesting that this new pathway may play an important role in the biological action of PGF2alpha on these cells.


Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/fisiologia , Dinoprosta/farmacologia , Período Pós-Parto/fisiologia , Contração Uterina/fisiologia , Animais , Proteínas Quinases Dependentes de Cálcio-Calmodulina/análise , Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Células Cultivadas , Ativação Enzimática , Feminino , Flavonoides/farmacologia , Quinases de Proteína Quinase Ativadas por Mitógeno , Miométrio/citologia , Miométrio/enzimologia , Toxina Pertussis , Fosforilação , Plasmídeos , Gravidez , Inibidores de Proteínas Quinases , Proteínas Quinases/análise , Proteínas Quinases/fisiologia , Proteínas Serina-Treonina Quinases/metabolismo , Proteínas Proto-Oncogênicas/metabolismo , Proteínas Proto-Oncogênicas c-raf , Ratos , Ratos Wistar , Ritodrina/farmacologia , Tocolíticos/farmacologia , Tirosina/metabolismo , Fatores de Virulência de Bordetella/farmacologia
11.
J Biol Chem ; 272(7): 4606-12, 1997 Feb 14.
Artigo em Inglês | MEDLINE | ID: mdl-9020189

RESUMO

Phosducin, which tightly binds betagamma-subunits of heterotrimeric G-proteins, has been conjectured to play a role in regulating second messenger signaling cascades, but to date its specific function has not been elucidated. Here we demonstrate a potential role for phosducin in regulating olfactory signal transduction. In isolated olfactory cilia certain odorants elicit a rapid and transient cAMP response, terminated by a concerted process which requires the action of two protein kinases, protein kinase A (PKA) and a receptor-specific kinase (GRK3) (Schleicher, S., Boekhoff, I. Arriza, J., Lefkowitz, R. J., and Breer, H. (1993) Proc. Natl. Acad. Sci. U. S. A. 90, 1420-1424). The mechanism of action of GRK3 involves a Gbetagamma-mediated translocation of the kinase to the plasma membrane bound receptors (Pitcher, J. A., Inglese, J., Higgins, J. B. , Arriza, J. L., Casey, P. J., Kim, C., Benovic, J. L., Kwatra, M. M. , Caron, M. G., and Lefkowitz, R. J. (1992) Science 257, 1264-1267). A protein with a molecular mass of 33 kDa that comigrates on SDS gels with recombinant phosducin and which is immunoreactive with phosducin antibodies is present in olfactory cilia. Recombinant phosducin added to permeabilized olfactory cilia preparations strongly inhibits termination of odorant-induced cAMP response and odorant-induced membrane translocation of GRK3. In addition, the cAMP analogue dibutyryl cAMP stimulates membrane targeting of the receptor kinase. This effect is presumably due to PKA-mediated phosphorylation of phosducin, which diminishes its affinity for binding to the Gbetagamma-subunit, thereby making Gbetagamma available to function as a membrane anchor for GRK3. A specific PKA inhibitor blocks the odorant-induced translocation of the receptor kinase. Consistent with this formulation, a non-phosphorylatable mutant of phosducin (phosducin Ser-73 --> Ala) is an even more effective inhibitor of desensitization and membrane targeting of GRK3 than the wild-type protein. A phosducin mutant that mimics phosphorylated phosducin (phosducin Ser-73 --> Asp) lacks this property and in fact recruits GRK3 to the membrane and potentiates desensitization. These results suggest that phosducin may act as a phosphorylation-dependent switch in second messenger signaling cascades, regulating the kinetics of desensitization processes by controlling the activity of Gbetagamma-dependent GRKs.


Assuntos
Proteínas do Olho/metabolismo , Condutos Olfatórios/metabolismo , Fosfoproteínas/metabolismo , Proteínas Serina-Treonina Quinases , Transdução de Sinais , Animais , Bovinos , AMP Cíclico/metabolismo , Proteínas do Olho/genética , Quinase 3 de Receptor Acoplado a Proteína G , Reguladores de Proteínas de Ligação ao GTP , Humanos , Proteínas de Membrana/metabolismo , Mutagênese Sítio-Dirigida , Fosfoproteínas/genética , Fosforilação , Ratos , Ratos Sprague-Dawley , Receptores Proteína Tirosina Quinases/metabolismo
12.
Artigo em Inglês | MEDLINE | ID: mdl-9344257

RESUMO

G-protein-coupled receptors that mediate cellular responses to a variety of humoral, endothelial-, or platelet-derived substances are able to stimulate MAP kinase activity. In transfected model systems, G-protein-coupled receptors that couple to pertussis toxin-insensitive G proteins of the Gq/11 family mediate this activation predominantly via a PKC-dependent mechanism. In contrast, activation of MAP kinase by receptors that couple to pertussis toxin-sensitive Gi proteins is PKC-independent and requires downstream activation of the low-molecular-weight G protein, Ras. This pathway can be inhibited by coexpression of peptides that sequester Gbetagamma subunits, and is mimicked by overexpression of Gbetagamma subunits. This Ras-dependent MAP kinase activation requires tyrosine phosphorylation of "docking proteins," including the shc adapter protein, and depends upon recruitment of Grb2/Sos1 complexes to the plasma membrane, thus resembling the pathway of MAP kinase activation employed by the receptor tyrosine kinases. Other molecules, including PI-3-kinases and phosphotyrosine phosphatases, probably also contribute to Gbetagamma-subunit-mediated assembly of a mitogenic signaling complex. Identification of the G-protein-coupled, receptor-regulated tyrosine kinase(s), and the means by which the mitogenic signaling complex is assembled at the plasma membrane, remain subjects of further study.


Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Fosfoproteínas , Receptores de Superfície Celular/metabolismo , Animais , Proteínas Sanguíneas/metabolismo , Ativação Enzimática , Humanos , Fosforilação , Transdução de Sinais , Tirosina/metabolismo , Proteínas ras/metabolismo
14.
J Neurochem ; 66(3): 1234-9, 1996 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-8769889

RESUMO

Phosphatidylinositol 4,5-bisphosphate (PIP2) is absolutely required for the ADP-ribosylation factor-stimulated phospholipase D (PLD) activity. In the present study, partially purified rat brain PLD was found to be activated by another PLD activator, RhoA, when PIP2, but not other acidic phospholipids, was included in vesicles comprising phosphatidylethanolamine (PE) and the PLD substrate phosphatidyicholine (PC) (PE/PC vesicles), demonstrating the absolute requirement of PIP2 for the RhoA-stimulated PLD activation, too. It is interesting that the RhoA-dependent PLD activity in the partially purified preparation was drastically decreased after the preparation was incubated with and separated from PE/PC vesicles containing PIP2. The PLD activity was extracted by higher concentrations of NaCl from the vesicles containing PIP2 that were incubated with and then separated from the partially purified PLD preparation. These results demonstrate that RhoA-dependent PLD binds to PE/PC vesicles with PIP2. The degree of binding of the RhoA-dependent PLD activity to the vesicles was totally dependent on the amount of PIP2 in the vesicles and correlated well with the extent of the enzyme activation. Further-more, it was found that a recombinant peptide of the pleckstrin homology domain of beta-adrenergic receptor kinase fused to glutathione S-transferase, which specifically binds to PIP2, inhibited the PIP2-stimulated, RhoA-dependent PLD activity in a concentration-dependent manner. From these results, it is concluded that in vitro rat brain PLD translocates to the vesicles containing PIP2, owing to its specific interaction with PIP2, to access its substrate PC, thereby catalyzing the hydrolysis of PC. PLD appears to localize exclusively on plasma membranes of cells and tissues. An aminoglycoside, neomycin, that has high affinity for PIP2 effectively extracted the RhoA-dependent PLD activity from rat brain membranes. This indicates that PIP2 serves as an anchor to localize PLD on plasma membranes in vivo.


Assuntos
Proteínas de Ligação ao GTP/fisiologia , Fosfatidilinositol 4,5-Difosfato/metabolismo , Fosfolipase D/metabolismo , Fatores de Ribosilação do ADP , Animais , Transporte Biológico , Ratos , Proteína rhoA de Ligação ao GTP
15.
Proc Natl Acad Sci U S A ; 92(20): 9284-7, 1995 Sep 26.
Artigo em Inglês | MEDLINE | ID: mdl-7568118

RESUMO

The mechanism of mitogen-activated protein (MAP) kinase activation by pertussis toxin-sensitive Gi-coupled receptors is known to involve the beta gamma subunits of heterotrimeric G proteins (G beta gamma), p21ras activation, and an as-yet-unidentified tyrosine kinase. To investigate the mechanism of G beta gamma-stimulated p21ras activation, G beta gamma-mediated tyrosine phosphorylation was examined by overexpressing G beta gamma or alpha 2-C10 adrenergic receptors (ARs) that couple to Gi in COS-7 cells. Immunoprecipitation of phosphotyrosine-containing proteins revealed a 2- to 3-fold increase in the phosphorylation of two proteins of approximately 50 kDa (designated as p52) in G beta gamma-transfected cells or in alpha 2-C10 AR-transfected cells stimulated with the agonist UK-14304. The latter response was pertussis toxin sensitive. These proteins (p52) were also specifically immunoprecipitated with anti-Shc antibodies and comigrated with two Shc proteins, 46 and 52 kDa. The G beta gamma- or alpha 2-C10 AR-stimulated p52 (Shc) phosphorylation was inhibited by coexpression of the carboxyl terminus of beta-adrenergic receptor kinase (a G beta gamma-binding pleckstrin homology domain peptide) or by the tyrosine kinase inhibitors genistein and herbimycin A, but not by a dominant negative mutant of p21ras. Worthmannin, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K) inhibited phosphorylation of p52 (Shc), implying involvement of PI3K. These results suggest that G beta gamma-stimulated Shc phosphorylation represents an early step in the pathway leading to p21ras activation, similar to the mechanism utilized by growth factor tyrosine kinase receptors.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas Adaptadoras de Transporte Vesicular , Proteínas de Ligação ao GTP/metabolismo , Proteínas/metabolismo , Receptores Adrenérgicos alfa 2/fisiologia , Androstadienos/farmacologia , Animais , Benzoquinonas , Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Linhagem Celular , Chlorocebus aethiops , Ativação Enzimática , Inibidores Enzimáticos/farmacologia , Proteínas de Ligação ao GTP/biossíntese , Genisteína , Humanos , Isoflavonas/farmacologia , Rim , Cinética , Lactamas Macrocíclicas , Substâncias Macromoleculares , Toxina Pertussis , Fosfatos/metabolismo , Fosfatidilinositol 3-Quinases , Fosforilação , Fosfotransferases (Aceptor do Grupo Álcool)/antagonistas & inibidores , Fosfotransferases (Aceptor do Grupo Álcool)/metabolismo , Proteínas Tirosina Quinases/antagonistas & inibidores , Proteínas Proto-Oncogênicas p21(ras)/metabolismo , Quinonas/farmacologia , Receptores Adrenérgicos alfa 2/biossíntese , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/metabolismo , Rifabutina/análogos & derivados , Proteínas Adaptadoras da Sinalização Shc , Proteína 1 de Transformação que Contém Domínio 2 de Homologia de Src , Acetato de Tetradecanoilforbol/farmacologia , Transfecção , Fatores de Virulência de Bordetella/farmacologia , Wortmanina
16.
Nature ; 376(6543): 781-4, 1995 Aug 31.
Artigo em Inglês | MEDLINE | ID: mdl-7651538

RESUMO

Mitogen-activated protein (MAP) kinases mediate the phosphorylation and activation of nuclear transcription factors that regulate cell growth. MAP kinase activation may result from stimulation of either tyrosine-kinase (RTK) receptors, which possess intrinsic tyrosine kinase activity, or G-protein-coupled receptors (GPCR). RTK-mediated mitogenic signalling involves a series of SH2- and SH3-dependent protein-protein interactions between tyrosine-phosphorylated receptor, Shc, Grb2 and Sos, resulting in Ras-dependent MAP kinase activation. The beta gamma subunits of heterotrimeric G proteins (G beta gamma) also mediate Ras-dependent MAP kinase activation by an as-yet unknown mechanism. Here we demonstrate that activation of MAP kinase by Gi-coupled receptors is preceded by the G beta gamma-mediated tyrosine phosphorylation of Shc, leading to an increased functional association between Shc, Grb2 and Sos. Moreover, disruption of the Shc-Grb2-Sos complex blocks G beta gamma-mediated MAP kinase activation, indicating that G beta gamma does not mediate MAP kinase activation by a direct interaction with Sos. These results indicate that G beta gamma-mediated MAP kinase activation is initiated by a tyrosine phosphorylation event and proceeds by a pathway common to both GPCRs and RTKs.


Assuntos
Proteínas Adaptadoras de Transdução de Sinal , Proteínas Adaptadoras de Transporte Vesicular , Proteínas de Ligação ao GTP/metabolismo , Proteínas Quinases/metabolismo , Receptores Proteína Tirosina Quinases/metabolismo , Transdução de Sinais , Linhagem Celular , Ativação Enzimática , Fator de Crescimento Epidérmico/metabolismo , Proteína Adaptadora GRB2 , Proteínas de Membrana/metabolismo , Fosforilação , Proteínas/metabolismo , Proteínas Recombinantes/metabolismo , Proteínas Adaptadoras da Sinalização Shc , Proteínas Son Of Sevenless , Tirosina/metabolismo
17.
Nihon Yakurigaku Zasshi ; 106(1): 1-9, 1995 Jul.
Artigo em Japonês | MEDLINE | ID: mdl-7590518

RESUMO

A pleckstrin homology (PH) domain is an approximately 100 amino acid region of sequence homology present in numerous proteins involved in signal transduction and growth control. The three dimensional structures of several PH domains demonstrate that they consist of a beta-barrel of seven antiparallel beta-sheets and a carboxyl-terminal amphiphilic alpha-helix. Several ligands capable of binding to PH domains have been identified including phosphatidylinositol 4,5-bisphosphate and the beta gamma subunits of heterotrimeric G proteins, which bind to the amino and carboxyl-termini of the PH domain, respectively. Furthermore, several isoforms of protein kinase C appear to bind to some PH domains. A general function of PH domains may be to anchor PH domain-containing proteins to the appropriate membrane-compartment. The membrane localization of PH domain-containing proteins may require cooperative multiple ligand binding to the PH domain. Finally, the heterogeneity of sequences among various PH domains may prove to be the basis for differences in the regulation and specificity of PH domain-ligand interaction in a fashion similar to SH2 and SH3 domains. The function of the PH domain and the mechanisms of PH domain action seem to be quite complex.


Assuntos
Homologia de Sequência de Aminoácidos , Animais , Sítios de Ligação , Ligantes , Ligação Proteica , Proteína Quinase C/metabolismo , Proteínas , Transdução de Sinais
18.
J Biol Chem ; 270(28): 17000-5, 1995 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-7622521

RESUMO

The beta gamma subunits of heterotrimeric G proteins (G beta gamma) play a variety of roles in cellular signaling, one of which is membrane targeting of the beta-adrenergic receptor kinase (beta ARK). This is accomplished via a physical interaction of G beta gamma and a domain within the carboxyl terminus of beta ARK which overlaps with a pleckstrin homology (PH) domain. The PH domain of beta ARK not only binds G beta gamma but also interacts with phosphatidylinositol 4,5-bisphosphate (PIP2). Based on previous mapping of the G beta gamma binding region of beta ARK, and conserved residues within the PH domain, we have constructed a series of mutants in the carboxyl terminus of beta ARK in order to determine important residues involved in G beta gamma and PIP2 binding. To examine the effects of mutations on G beta gamma binding, we employed three different methodologies: direct G beta gamma binding to GST fusion proteins; the ability of GST fusion proteins to inhibit G beta gamma-mediated beta ARK translocation to rhodopsin-enriched rod outer segments; and the ability of mutant peptides expressed in cells to inhibit G beta gamma-mediated inositol phosphate accumulation. Direct PIP2 binding was also assessed on mutant GST fusion proteins. Ala residue insertion following Trp643 completely abolished the ability of beta ARK to bind G beta gamma, suggesting that a proper alpha-helical conformation is necessary for the G beta gamma.beta ARK interaction. In contrast, this insertional mutation had no effect on PIP2 binding. Both G beta gamma binding and PIP2 binding were abolished following Ala replacement of Trp643, suggesting that this conserved residue within the last subdomain of the PH domain is crucial for both interactions. Other mutations also produced differential effects on the physical interactions of the beta ARK carboxyl terminus with G beta gamma and PIP2. These results suggest that the last PH subdomain and its neighboring sequences within the carboxyl terminus of beta ARK, including Trp643, Leu647, and residues Lys663-Arg669, are critical for G beta gamma binding while Trp643 and residues Asp635-Glu639 are important for the PH domain to form the correct structure for binding to PIP2.


Assuntos
Proteínas Sanguíneas/metabolismo , Proteínas Quinases Dependentes de AMP Cíclico/metabolismo , Proteínas de Ligação ao GTP/fisiologia , Fosfatos de Fosfatidilinositol/metabolismo , Fosfoproteínas , Sequência de Aminoácidos , Animais , Sítios de Ligação , Transporte Biológico , Bovinos , Humanos , Dados de Sequência Molecular , Mutação , Fosfatidilinositol 4,5-Difosfato , Proteínas Recombinantes de Fusão/metabolismo , Quinases de Receptores Adrenérgicos beta
19.
J Biol Chem ; 270(28): 16495-8, 1995 Jul 14.
Artigo em Inglês | MEDLINE | ID: mdl-7622449

RESUMO

The receptors for insulin-like growth factor 1 (IGF1) and insulin are related heterotetrameric proteins which, like the epidermal growth factor (EGF) receptor, possess intrinsic ligand-stimulated tyrosine protein kinase activity. In Rat 1 fibroblasts, stimulation of mitogen-activated protein (MAP) kinase via the IGF1 receptor and the Gi-coupled receptor for lysophosphatidic acid (LPA), but not via the EGF receptor, is sensitive both to pertussis toxin treatment and to cellular expression of a specific G beta gamma subunit-binding peptide. The IGF1, LPA, and EGF receptor-mediated signals are all sensitive to inhibitors of tyrosine protein kinases, require p21ras activation, and are independent of protein kinase C. These data suggest that some tyrosine kinase growth factor receptors (e.g. IGF1 receptor) and classical G protein-coupled receptors (e.g. LPA receptor) employ a similar mechanism for mitogenic signaling that involves both tyrosine phosphorylation and G beta gamma subunits derived from pertussis toxin-sensitive G proteins.


Assuntos
Proteínas Quinases Dependentes de Cálcio-Calmodulina/metabolismo , Proteínas de Ligação ao GTP/fisiologia , Receptor IGF Tipo 1/fisiologia , Animais , Células Cultivadas , Ativação Enzimática , Toxina Pertussis , Proteína Quinase C/fisiologia , Ratos , Fatores de Virulência de Bordetella/farmacologia
20.
J Biol Chem ; 270(22): 12984-9, 1995 Jun 02.
Artigo em Inglês | MEDLINE | ID: mdl-7768889

RESUMO

Pleckstrin homology (PH) domains are 90-110 amino acid regions of protein sequence homology that are found in a variety of proteins involved in signal transduction and growth control. We have previously reported that the PH domains of several proteins, including beta ARK1, PLC gamma, IRS-1, Ras-GRF, and Ras-GAP, expressed as glutathione S-transferase fusion proteins, can reversibly bind purified bovine brain G beta gamma subunits in vitro with varying affinity. To determine whether PH domain peptides would behave as antagonists of G beta gamma subunit-mediated signal transduction in intact cells, plasmid minigene constructs encoding these PH domains were prepared, which permit transient cellular expression of the peptides. Pertussis toxin-sensitive, G beta gamma subunit-mediated inositol phosphate (IP) production was significantly inhibited in COS-7 cells transiently coexpressing the alpha 2-C10 adrenergic receptor (AR) and each of the PH domain peptides. Pertussis toxin-insensitive, Gq alpha subunit-mediated IP production via coexpressed M1 muscarinic acetylcholine receptor (M1 AChR) was attenuated only by the PLC gamma PH domain peptide, suggesting that the inhibitory effect of most of the PH domain peptides was G beta gamma subunit-specific. Stimulation of the mitogen-activated protein (MAP) kinase pathway by Gi-coupled receptors in COS-7 cells has been reported to require activation of p21ras and to be independent of protein kinase C. Since several proteins involved in activation contain PH domains, the effect of PH domain peptide expression on alpha 2-C10 AR-mediated p21ras-GTP exchange and MAP kinase activation as well as direct G beta gamma subunit-mediated activation of MAP kinase was determined. In each assay, coexpression of the PH domain peptides resulted in significant inhibition. Increasing G beta gamma subunit expression surmounted PH domain peptide-mediated inhibition of MAP kinase activation. These data suggest that the PH domain peptides behave as specific antagonists of G beta gamma-mediated signaling in intact cells and that interactions between PH domains and G beta gamma subunits or structurally related proteins may play a role in the activation of mitogenic signaling pathways by G protein-coupled receptors.


Assuntos
Proteínas Sanguíneas/metabolismo , Proteínas de Ligação ao GTP/metabolismo , Fosfoproteínas , Receptores de Superfície Celular/metabolismo , Transdução de Sinais , Animais , Proteínas Sanguíneas/genética , Bovinos , Células Cultivadas , Ativação Enzimática , Fosfatos de Inositol/biossíntese , Proteína Oncogênica p21(ras)/metabolismo , Proteínas Quinases/metabolismo , Homologia de Sequência de Aminoácidos
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