RESUMO
This study evaluated the efficacy of PDT in photoinactivation of Candida species using methylene blue (MB) and irradiation with a diode laser (660nm, 40mW). Suspensions of Candida species were obtained containing 10(6)cfu/ml, transferred to 96-holes plates and exposed to 03 doses of laser light (60J/cm(2), 120J/cm(2), 180J/cm(2)) in the presence of MB. Additional suspensions were treated with only the MB, the laser light or with 0.85% saline (control groups). After the treatments, 1µl aliquot of the suspensions was plated in duplicate on SDA. The plates were incubated at 37°C for 24-48h and after this period there was the counting of colonies (cfu/ml). The three evaluated doses determined meaningful inactivation of Candida spp. (p<0.05). The 180J/cm(2) dose was the most effective, inactivating 78% of cfu/ml. At a dose of 180J/cm(2)C. albicans was the most susceptible specie. PDT has demonstrated effectiveness in the inactivation of Candida spp.
