[Surgical treatment of a complication of Crohn's disease of the duodenum]. / Chirurgická lécba komplikací Crohnovy choroby duodena.

In 1995-1997 at the First Surgical Clinic, Medical Faculty, Palacký University and Faculty Hospital Olomouc three patients were treated on account of obstruction of the pyloroduodenal region caused by Crohn's disease. The condition was treated by anastomosis, possibly with vagotomy, depending on the site of stenosis. Clinical, laboratory and endoscopic control examinations after a 6-month to 3-year interval revealed good results of this approach.

[Surgical possibilities in the treatment of ulcerative colitis]. / Chirurgické moznosti lécení ulcerózní kolitidy.

During the five-year period between 1992-1996 at the First Surgical Clinic of the Medical Faculty, Palacký University and Faculty Hospital in Olomouc 22 patients were operated on account of ulcerative colitis. Twelve patients were subjected to primary operations on account of acute complications of the disease, ten patients are in the group of elective operations. As the incidence of ulcerative colitis in our population is not high there is little experience with treatment of this disease and ulcerative colitis is still a problem for gastroenterologists as well as surgeons. The authors draw attention to two factors which have an impact on surgical treatment of ulcerative colitis: 1. optimal time for indication of surgical treatment calls for treatment of patients in a specialized gastroenterological department, 2. Surgical treatment calls for a specialised team linked with the gastroenterological department concerned with treatment of ulcerative colitis.

Isolation and purification of bacterial proteinases by means of autofocusing.

Autofocusing was used for the isolation and purification of neutral and alkaline proteinases from fermentation broth, after separation of cells. The yield of proteinases achieved was 19-78%, and was inversely proportional to the degree of purification, which varied from 3.0 to 7.9. Because of considerable losses of enzyme activity and the long duration of the process, autofocusing seems to be a non-economic technique for industrial isolation of relatively cheap enzymes.