20-hydroxyecdysone as a human lymphocyte and neutrophil modulator: In vitro evaluation.

20-hydroxyecdysone (1 microM) was found to activate in vitro T-cell CD2 presentation, which is suppressed both in secondary immunodeficient persons and pharmacologically by increasing intracellular cAMP levels. The compound was found to act like a synthetic psycho-immunomodulator 1-oxy-4-oxoadamantane (1 microM) and to exceed the effects of the thymomimetic agent levamisol (1 microM). In addition, 20-hydroxyecdysone (1 microM) was also revealed to modulate the fluoride-stimulated respiratory burst of human neutrophils in the same manner as water soluble antioxidants. Arch.

[The ecdysteroid fraction of the above-ground portion of Serratula coronata L. in the spontaneous E-rosette formation reaction and the agar migration test in vitro]. / Ekdisteroidnaia fraktsiia nadzemnoi chasti Serratula coronata L. v reaktsii spontannogo E-rozetkoobrazovaniia i agarmigratsionnom teste in vitro.

Reaction of E-rosette formation and agar migration test in vitro showed that small concentrations (7.5.10(-13) -7.5.10(-8) M) of phytosteroid ecdysterone (20-hydroxyecdysone) induce the activation of human lymphocytes. The effect of higher concentrations (7.5.10(-7) -7.5.10(-5) M) is less pronounced (two-phase effect).