Factors influencing pre-ovulatory follicle diameter and weaning-to-ovulation interval in spontaneously ovulating sows in tropical environment.

Follicle development and timing of ovulation are indicators of the reproductive performance of sows. The present study aimed to determine factors influencing pre-ovulatory follicle diameter and weaning-to-ovulation interval (WOI) in spontaneously ovulating sows in tropical climates with special emphasis on breed, parity and backfat thickness at weaning. In total, 80 sows were included in the study. Follicle development was determined by using transrectal real-time B-mode ultrasonography every 6 hr after standing oestrus. Weaning-to-oestrous interval (WEI), oestrous-to-ovulation interval (EOI), WOI and the diameter of graafian follicles were investigated in relation to breed, parity number (1, 2-3 and 4-7) and backfat thickness (low, moderate and high) of sows. Overall, WEI, EOI, WOI and the pre-ovulatory follicle diameter were 92.5 ± 21.6 hr, 64.3 ± 19.3 hr, 156.3 ± 29.1 hr and 10.3 ± 2.0 mm, respectively. Pre-ovulatory follicle size was smaller in primiparous sows compared with sows of greater parity, 4-7 (9.7 ± 0.51 and 11.7 ± 0.52 mm, respectively, p < .05). Weaning-to-ovulation interval was positively correlated with WEI (r = 0.75, p < .001) and EOI (r = 0.66, p < .001), but negatively correlated with size of the graafian follicle (r = -0.34, p < .01). Sows with a shorter WEI had a larger pre-ovulatory follicle diameter (at 64 hr after oestrus) (r = -0.37, p < .01). Sows with low backfat thickness had a WOI 23.4 hr longer than those with moderate backfat thickness (p < .05) and 17.6 hr longer than sows with a high backfat thickness (p = .140). The follicle diameter in primiparous sows with high backfat thickness (11.7 ± 1.1 mm) was higher than in those with low (8.9 ± 0.7 mm, p < .05) or moderate (8.6 ± 0.8, p < .05) backfat thickness. In conclusion, factors influencing follicle diameter and WOI in sows included parity number and backfat thickness at weaning. The impact of backfat thickness on follicle diameter, WEI and WOI was most pronounced in primiparous sows.

Reproductive performance of weaned sows after single fixed-time artificial insemination under a tropical climate: Influences of season and insemination technique.

We evaluated the reproductive performance of sows after single fixed-time AI under a tropical climate and investigated the influences of season and insemination technique on the efficacy of single fixed-time AI. After weaning, the sows were divided into CONTROL (n = 212) and FIXED-TIME (n = 212) groups. Sows in the CONTROL group were inseminated at 12 and 36 h after the onset of oestrus, while sows in the FIXED-TIME group were administered 10 µg of GnRH at 72 h after weaning and were inseminated 32 h later. Reproductive performance parameters, including total born, born alive, mummified foetuses and stillborn piglets per litter, piglet birth weight, variation of piglet birth weight within litter, regular return-to-oestrus and farrowing rate, were compared between the two groups. Season was classified into two groups: cool (n = 170) and hot (n = 254), and insemination technique was classified into two groups: conventional AI (n = 171) and intra-uterine insemination (IUI) with a reduced number of spermatozoa (n = 253). On average, regular return-to-oestrus (3.3 vs. 5.6%, P > 0.05) and farrowing rates (92.8 vs. 88.1%, P > 0.05) did not differ between CONTROL and FIXED-TIME groups. However, the total born and born alive piglets per litter in the FIXED-TIME were lower than in the CONTROL group (12.0 vs. 12.8 piglets/litter; P = 0.030 and 11.3 vs. 12.2 piglets/litter, P = 0.007). Interestingly, the number of total born piglets in the FIXED-TIME group was lower than in the CONTROL group only in the sows inseminated in the hot season (11.7 ±â€¯0.32 and 12.9 ±â€¯0.31, respectively, P = 0.005). Piglet birth weight, variation of piglet birth weight within litter, number of piglets at weaning and body weight of piglets at weaning did not differ between groups, irrespective of the season (P > 0.05). The total number of piglets born per litter in the FIXED-TIME group was lower than that in the CONTROL group in sows inseminated via IUI (11.7 ±â€¯0.32 and 12.9 ±â€¯0.32, respectively, P = 0.013), but not in sows inseminated using conventional AI (12.7 ±â€¯0.42 and 12.5 ±â€¯0.41, respectively, P = 0.772). Single fixed-time AI could be successfully performed in sows under a tropical climate, with a promising reproductive performance. However, a decreased litter size at birth after single fixed-time AI was observed when insemination was performed in the hot season. Moreover, single fixed-time AI using IUI with a reduced number of spermatozoa also decreased litter size at birth.

Factors affecting estrus and ovulation time in weaned sows with induced ovulation by GnRH administration in different seasons.

Follicle development in post-weaning sows is influenced by various factors. To control ovulation time using hormone, factors that influence ovulation should be investigated. The present study was performed to evaluate the effect of GnRH (buserelin) administration in relation to season and sow parameters on ovulation time in weaned sows. Seventy-seven weaned sows were divided into the following groups: control (hot season, n=21; cool season, n=16) and treatment (hot season, n=22; cool season, n=18). Sows were kept in a close house equipped with an evaporative cooling system. Ovulation time was determined every 6 hr using transrectal ultrasonography. Administration of 10 µg buserelin at 72 hr after weaning affected estrus-to-ovulation interval (EOI) and weaning-to-ovulation interval (WOI) in sows (P<0.05). The percentage of sows that ovulated between 44-56 hr after injection was higher in the cool season than in hot season (P<0.05). Weaning-to-estrus interval (WEI) and injection-to-estrus interval (IEI) were affected by season (P<0.05). Body condition score (BCS) of sows influenced EOI (P<0.01). Sows with low backfat thickness, lactation length <20 days, or litter weight ≥67 kg, had delayed injection-to-ovulation interval (P<0.05). In conclusions, buserelin administration (10 µg, at 72 hr after weaning) advanced ovulation. Hot season prolonged ovulation time. Sows that were weaned with lactation length of at least 20 days, litter weight less than 67 kg, or BCS of at least 3, had better responses to buserelin injection. High backfat reserve after weaning is important for ovulation induction response by buserelin injection.