Phytochemical Characterization, Antioxidant Activity, and Cytotoxicity of Methanolic Leaf Extract of Chlorophytum Comosum (Green Type) (Thunb.) Jacq.

Chlorophytum genus has been extensively studied due to its diverse biological activities. We evaluated the methanolic extract of leaves of Chlorophytum comosum (Green type) (Thunb.) Jacques, the species that is less studied compared to C. borivilianum. The aim was to identify phytoconstituents of the methanolic extract of leaves of C. comosum and biological properties of its different fractions. Water fraction was analyzed with matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectrometry. Nineteen compounds belonging to different chemical classes were identified in the methanolic extract of leaves of C. comosum (Green type) (Thunb.) Jacques. In addition to several fatty acids, isoprenoid and steroid compounds were found among the most abundant constituents. One of the identified compounds, 4'-methylphenyl-1C-sulfonyl-ß-d-galactoside, was not detected earlier in Chlorophytum extracts. The water fraction was toxic to HeLa cells but not to Vero cells. Our data demonstrate that methanolic extract of leaves of C. comosum can be a valuable source of bioactive constituents. The water fraction of the extract exhibited promising antitumor potential based on a high ratio of HeLa vs. Vero cytotoxicity.

The first water-soluble polynuclear metallamacrocyclic Sr(ii)-Cu(ii) complex based on simple glycinehydroximate ligands.

Recently, aminohydroximate ligands have found wide applications in the fascinating class of polynuclear metallamacrocyclic compounds named 15-MC-5 metallacrowns. The enhanced interest in water-soluble aminohydroximate Ln(iii)-Cu(ii) complexes is largely due to their rich coordination chemistry, diverse properties and ease of synthesis. We examined glycinehydroxamic acid as a simple ligand for the preparation of the first water-soluble polynuclear metallamacrocyclic Sr(ii)-Cu(ii) compound. The complex Sr(H2O)3[15-MCCuGlyha-5](Cl)2 was synthesized and characterized structurally and spectroscopically. The single-crystal structure reveals the classic metallamacrocyclic 15-MC-5 configuration. The Sr(ii) ion is located at the center of the 15-MCCu(II)Glyha-5 ring and coordinated by five oxygen atoms of the cycle in the equatorial plane and an additional three oxygen atoms of the water molecules at apical positions. Detailed DFT and QT AIM studies were carried out for the hydrated isoelectronic Sr[15-MCCuGlyha-5]2+ and Y[15-MCCuGlyha-5]3+ systems. The ionic contribution to the metal-ligand interactions appears to be higher for the Sr(ii) derivative despite the smaller charge separation. DFT calculations suggest a thermodynamically favorable substitution of the Sr(ii) central ion with Y(iii). Indeed, it was shown experimentally that the strontium ion can be easily replaced by yttrium. Preliminary cytotoxic studies revealed the low toxicity of the strontium complex and its yttrium analogue, so the water-soluble Sr(ii) and Y(iii) metallacrowns can be further investigated as possible platforms for the development of new 90Sr and 90Y radiotherapy drugs.