RESUMO
The article deals with the results of HLA-typing of 1829 patients on loci HLA-A, HLA-B, HLA-DRB1 using kits of LABType SSO reagents (One Lambada, USA). The indicators of each locus ambiguity are determined: locus HLA-A - 4.43%, locus HLA-B--4.43%, locus HLA-DRB1--0.16%. The list of "rare" alleles was applied to analyze 13 types of determined ambiguities on locus HLA-A, 27 types on locus HLA-B, 3 types on locus HLA-DRB1.
Assuntos
Alelos , Loci Gênicos , Antígenos HLA/genética , Teste de Histocompatibilidade/métodos , Análise de Sequência de DNA/métodos , Feminino , Teste de Histocompatibilidade/instrumentação , Teste de Histocompatibilidade/normas , Humanos , Masculino , Kit de Reagentes para Diagnóstico , Análise de Sequência de DNA/instrumentação , Análise de Sequência de DNA/normasRESUMO
HLA was typed from HLA-A, HLA-B, HLA-DRB1 loci (to the second place) in 443 patients, by applying the LABType SSO reagent kits (One Lambda, USA). The findings were analyzed using the software Arlequin version 3.1. There were no deviations from the Hardy-Weinberg law. Overall, the authors identified 16, 27, and 13 allelic variants ofHLA-A, HLA-B, and HLA-DRB1 loci, respectively. There were also 4 most common haplotypes of HLA-A-B-DRBII: HLA-A *03-B*35-DRB1 *01 (4.29%), HLA-A *01-B*08-DRBl *03 (3.5%), HLA-A*03-B*07-DRBI *15 (3.37%), and HLA-A *02-B*07-DRBI *15 (2.93%).
Assuntos
Antígenos HLA/metabolismo , Teste de Histocompatibilidade/normas , Grupos Populacionais , Doadores de Sangue , Frequência do Gene , Genética Populacional , Antígenos HLA/genética , Haplótipos , Transplante de Células-Tronco Hematopoéticas , Teste de Histocompatibilidade/métodos , Humanos , Reação em Cadeia da Polimerase , Kit de Reagentes para Diagnóstico/normas , SoftwareRESUMO
A homogeneous protein LSF-2 preparation was extracted from the cultural fluid of Bordetella pertussis strains of the 1.0.3 serological type by means of precipitation with ammonium sulphate and electrofocussing; this preparation proved to produce a marked leukocytosis-stimulating and a weak toxic action of delayed type in experiments on animals. Intraperitoneal administration of 5 mug of the LSF-2 preparation caused a rise of leukocytosis in mice to 100,000 cells per 1 mm3, a delay in the gain in weight beginning from the 3rd day of the administration and a late death of the animals in 5% of cases. The LSF-2 preparation protected the mice in infection with a virulent pertussis strain No. 18323 in the amount of from 12 to 91%, depending on the immunizing dose; its ImD50 was equal to 2.0 -2.4 mug of protein. The results of investigations carried out permitted to assess the role of this substance in the formation of specific immunity in pertussis infection.
Assuntos
Toxinas Bacterianas/isolamento & purificação , Bordetella pertussis/análise , Leucocitose/induzido quimicamente , Vacina contra Coqueluche/isolamento & purificação , Animais , Antígenos de Bactérias/isolamento & purificação , Proteínas de Bactérias/isolamento & purificação , Peso Corporal , Camundongos , Vacinação , Coqueluche/prevenção & controleRESUMO
The results of the study revealed correlation between the reactogenicity of pertussis vaccines in epidemiological observations and the toxic properties of pertussis bacteria in experiment. Quantitative and qualitative differences were found between the toxic factors in pertussis bacteria depending on their type-specific serological activity. Serotype 1.0.3 microbes exhibit more pronounced toxicity which accounts for the greater reactogenicity of vaccines prepared from this serotype as compared with the preparation produced from the serotype 1.2.3. The obtained results suggest the necessity of considering the greater toxicity of the serotype 1.0.3 in the preparation of pertussis vaccines.