Energy balance in man measured by direct and indirect calorimetry.

In six 24-hr measurements of energy balance, direct and indirect calorimetry agreed within +/-3%, which is probably the range of experimental error. But in seven other 24-hr periods there was disagreement in the range of 8 to 23%, and these were usually days when the subjects ate much less than they spent metabolically. Our direct calorimeter is an insulated, water cooled suit. Continous measurements of O2 consumption and CO2 production provided data on metabolic expenditure (M) by indirect calorimetry. The 24-hr values for M matched the energy losses within +/-60 kcal (+/-3% of M) in four men who rested all day and lay down to sleep at night. Similar agreement was seen in one of the four who worked on a treadmill for 4 hr and stayed busy all day. but in another energy losses were 342 kcal greater than M (10% of M). When the experiments gave values for M minus the losses greater than +/-60 kcal, this is called "unmeasured energy". In further experiments, two subjects stayed awake for 24 hr, and their unmeasured energies were 279 and 393 kcal. The same two men, eating sparingly, also worked for 24 hr so as to double their resting metabolic expenditures; the unmeasured energies were even larger, 380 and 958 kcal. When they repeated the 24 hr of mild work, but ate nearly as much as they spent metabolically, one man was near energy balance, while the other showed an unmeasured energy of -363 kcal. Little heat storage was evident in these experiments; therefore, heat balance was present and energy balance should have been present. In the group of 13 experiments, it appeared that the greater the food deficit, the larger was the unmeasured energy (excess of metabolic expenditure over loss of energy).

Hana kai ii: a 17-day dry saturation dive at 18.6 ATA. II. Energy balance.

Since previous saturation dives have caused loss of body weight despite apparently adequate-to-high food intake, a complete study of energy balance was undertaken during the saturation dive Hana Kai II. Over a 30-day period in the hyperbaric chamber (3 days of predive control, 1 day of compression, 16 days at 18.6 ATA, 7 days of decompression, and 3 days of postdive control), all food, urine, and feces for five men were analyzed by bomb calorimetry; 24-h energy expenditure (M) was measured from continuous VO2, VCO2, and urine N. Body weight was taken daily; body composition was assessed from density, total body water, and skinfold thickness. Food intake was high throughout the 30 days (about 3500 kcal/day) while fecal and urinary losses were a normal 6-8% of intake. Energy expenditure was increased a little by the hyperbaric condition, but averaged only 2431 kcal/day for the 30 days and yet there was an average loss of adipose tissue of 0.8 kg for each man for the entire period. Nitrogen balance was positive. There was no evidence of heat gain or loss. The energy balance, total fuel compared with energy expenditure, required an additional 919 kcal/man-day for 30 days, an unidentified term which is not measured by conventional techniques.