The effect of polyphenolic-polysaccharide conjugates from selected medicinal plants of Asteraceae family on the peroxynitrite-induced changes in blood platelet proteins.

Lots of plants belonging to Asteraceae family are very popular in folk medicine in Poland. These plants are also known as being rich in acidic polysaccharides, due to the presence of hexuronic acids or its derivatives. Our preliminary experiments have shown that the extract from Conyza canadensis L. possesses various biological activity, including antiplatelet, antiocoagulant and antioxidant properties. The aim of our study was to assess if macromolecular glycoconjugates from selected herbal plants of Asteraceae family: Achillea millefolium L., Arnica montana L., Echinacea purpurea L., Solidago virgaurea L., Chamomilla recutita (L.) Rauschert., and Conyza canadensis L. protect platelet proteins against nitrative and oxidative damage induced by peroxynitrite, which is responsible for oxidative/nitrative modifications of platelet proteins: the formation of 3-nitrotyrosine and carbonyl groups. These modifications may lead to changes of blood platelet functions and can have pathological consequences. The role of these different medicinal plants in the defence against oxidative/nitrative stress in human platelets is still unknown, therefore the oxidative damage to platelet proteins induced by peroxynitrite and protectory effects of tested conjugates by the estimation of carbonyl group level and nitrotyrosine formation (a marker of protein nitration) were studied in vitro. The antioxidative properties of the polyphenolic-polysaccharide conjugates from selected tested medicinal plants were also compared with the action of a well characterized antioxidative commercial polyphenol - resveratrol (3,4',5-trihydroxystilbene). The obtained results demonstrate that the compounds from herbal plants: A. millefolium, A. montana, E. purpurea, C. recutita, S. virgaurea, possess antioxidative properties and protect platelet proteins against peroxynitrite toxicity in vitro, similar to the glycoconjugates from C. canadensis. However, in the comparative studies, the polyphenolic-polysaccharide conjugates from selected tested medicinal plants were not found to be more effective antioxidant, than the solution of pure resveratrol.

Indirect (chronic) self-destructiveness and modes of suicide attempts.

INTRODUCTION: Suicides and suicide attempts may be considered as manifestations not only of direct self-destructiveness, but also of indirect (chronic) self-destructiveness. The aim of this work is to assess the relation between intensity of indirect self-destructiveness and suicide attempt modes. MATERIAL AND METHODS: The research was conducted among 147 persons after suicide attempts (114 women and 33 men) aged 14-33 years. The Polish version of the "Chronic Self-Destructiveness Scale" by Kelley (CS-DS) in Suchanska's adaptation was used. RESULTS: EXAMINED PERSONS APPLIED SEVERAL MODES OF COMMITTING SUICIDE: pharmacological drugs, exsanguination, hanging, jumping from a height, asphyxia, poisoning, and throwing oneself under a moving car. The most frequent are pharmacological drugs (42.31%) and exsanguination (25.60%); the rarest is throwing oneself under a car (1.28%). Relations between indirect self-destructiveness and the following suicide attempt modes were found: pharmacological drugs (0.366, p = 0.00001), poisoning (0.667, p = 0.01), and throwing oneself under a car (0.542, p = 0.04). There were found three clusters regarding the intensity of indirect self-destructiveness (low, medium and high). The largest differences concerned hanging, jumping from a height (medium CS-DS), poisoning and throwing oneself under a car (high CS-DS). There is a relation between indirect self-destructiveness and recurrence of suicide attempts (Kendall Tau = 0.217, p = 0.007); the intensity of indirect self-destructiveness differentiates the risk of consecutive suicide attempts (F = 2.871, p = 0.05). CONCLUSIONS: The implications of the findings are mainly of preventive and therapeutic nature. In the preventative aspect, the subject of interest should be not only persons with a tendency to the active form of indirect self-destructiveness, but also persons exhibiting its passive form.