[Testing the efficiency of Bacillus thuringien israelesis against mosquito larvae in Uzbekistan]. / Ispytaniia éffektivnosti Bacillus thuringiensis var. israelensis protiv lichinok komarov v Uzbekistane.

Experiments were conducted in the potential and neighboring malarious areas in the flood plains of the Tupalang River in the Sarias District, Surkhandarya Region, Uzbekistan. They have indicated that the numbers of larvae of pp. Culex and Aedes within the first 24 hours after treatment with BTI drastically reduced with all test doses (0.01-5.0 g/m2) and 92.3-100% larval deaths were recorded. At the same time the efficacy of the drugs retained for more than 7 days (100% death), i.e. throughout the observations. The second experiment have shown that all the test dosage forms of BTI drugs against An. superpictus larvae within 5 days after treatment of water reservoirs displayed virtually a 100% efficacy; on day 7, their efficacy slightly decreased, but remained rather high (75.4-99.7%). On day 14, drugs as granules and emulsions showed a noticeable reduction in their efficacy while the efficacy of powder-like drugs was in the range of 64.2-100%. The power-like drug Vectobac WDG in doses of 0.05-0.2 g/m2 exhibited the longest efficacy (87.6-100%). There was a noticeable increase in the numbers of mosquitoes in the control water reservoirs on days 7 and 14 of experiments.

[The use of polymerase chain reaction for detection of tetracycline resistance urogenital mycoplasma ]. / Primenenie polimeraznoi tsepnoi reaktsii dlia vyiavleniia teratsiklinustochivykh shtammov urogenital'nykh mikoplazm.

PCR was used for detecting tetracyclin resistance genes tet-M and tet-O in Mollicutes. The tet determinants were detected in U. urealyticum and M. hominis genomes in 60 and 57.14%, respectively. PCR was used to detect urogenital mycoplasma in patients with different urogenital infections. The rate of detection of U. urealyticum was 10.71-25.1% in males and 23.08-61.91% in females, for M. hominis these values were 6.25-11.45% and 3.85-22.99%, and for M. genitalium 6.25-29.64% and 4.76-30.0%, respectively. Hence, the detection rate of tet determinants in mycoplasma by PCR was high (7-60%) in all groups of patients.

[Detection of tetracycline- and erythromycin-resistant urogenital Mycoplasma strains using PCR]. / Vyiavlenie tetratsiklin- i éritromitsinustoichivykh shtammov urogenital'nykh mikoplasm s pomoshch'iu PTsR.

The use of PCR made it possible to obtain data on the occurrence of genital mycoplasms in subjects with different pathological states of their urogenital system. The detection rate of U.urealyticum was 10.7-25.1% in males and 23.0-61.9% in females. The detection rate of Mycoplasma hominis was 6.2-11.4% in males and 3.8-22.9% in females. The detection rate of M.genitalium was 6.2-29.6% in males and 4.7-30.0% in females. The study carried out by means of amplification test systems for the detection of genes controlling resistance to tetracycline (tet-M and tet-O) and to erythromycin (erm) demonstrated that U.urealyticum contained the tet-determinant in 60% of cases and M.hominis, in 57.14% of cases. As revealed with the use of PCR techniques, the occurrence of erythromycin-resistant Ureaplasma strains was not high and was equal to 2.1%.