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Objectives: It is feared that the serotype replacement of Streptococcus pneumoniae occurred by the introduction of pneumococcal vaccines as periodical inoculation leads to reduced efficacy of the approved vaccines and altered antimicrobial susceptibility. Methods: We determined serotypes of 351 S. pneumoniae isolates collected at a commercial clinical laboratory in Hokkaido prefecture, Japan, from December 2018 to February 2019 by using the polymerase chain reaction procedure of the US Centers for Disease Control and Prevention. Antimicrobial susceptibility and resistance gene profiles were also examined. Results: Vaccine coverage rates were 7.9% for 13-valent conjugate vaccine, and 32.5% for 23-valent polysaccharide vaccine, respectively. Non-typable strains were 19.7%. cpsA-positive isolates (group I), and null capsule clade (NCC)1, NCC2 and NCC3 (group II) comprised 31.3%, 28.4%, 32.8%, and 7.5% of the 69 non-typable strains, respectively. No penicillin-resistant/intermediate isolates were found; however, serotypes 35B and 15A/F showed low susceptibility to ß-lactams. Only five strains (1.4%) were levofloxacin-resistant, and all were from the older persons, and three strains were serotype 35B. Conclusion: The progression of serotype replacement in non-invasive pneumococcal infections has occurred during the post-vaccine era in Japan, and non-encapsulated isolates, such as NCC, have increased. Antimicrobial susceptibility is not worsened.
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Enterococcus faecalis and E. faecium are the major pathogens causing community- and healthcare-associated infections, with an ability to acquire resistance to multiple antimicrobials. The present study was conducted to determine the prevalence of virulence factors, drug resistance and its genetic determinants, and clonal lineages of E. faecalis and E. faecium clinical isolates in northern Japan. A total of 480 (426 E. faecalis and 54 E. faecium) isolates collected over a four-month period were analyzed. Three virulence factors promoting bacterial colonization (asa1, efaA, and ace) were more prevalent among E. faecalis (46-59%) than E. faecium, while a similar prevalence of enterococcal surface protein gene (esp) was found in these species. Between E. faecalis and E. faecium, an evident difference was noted for resistance to erythromycin, gentamicin, and levofloxacin and its responsible resistance determinants. Oxazolidinone resistance gene optrA and phenicol exporter gene fexA were identified in an isolate of E. faecalis belonging to ST480 and revealed to be located on a cluster similar to those of isolates reported in other Asian countries. The E. faecalis isolates analyzed were differentiated into 12 STs, among which ST179 and ST16 of clonal complex (CC) 16 were the major lineage. Nearly all the E. faecium isolates were assigned into CC17, which consisted of 10 different sequence types (STs), including a dominant ST17 containing multidrug resistant isolates and ST78 with isolates harboring the hyaluronidase gene (hyl). The present study revealed the genetic profiles of E. faecalis and E. faecium clinical isolates, with the first identification of optrA in ST480 E. faecalis in Japan.
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OBJECTIVES: Fluoroquinolone (FQ)- and third-generation cephalosporin-resistant Escherichia coli are increasing in Japan. In the early 2000s, the FQ-resistant E. coli clone ST131 increased in clinical settings worldwide. It frequently produces extended-spectrum ß-lactamases (ESBLs) such as CTX-M. This study aimed to explore the characteristics of FQ-resistant E. coli isolated in Japan during 2008-2009 and 2020. METHODS: We compared FQ-resistant E. coli clinical isolates from urine samples collected in 2020 (151 isolates) with a FQ-resistant E. coli collection isolated in 2008-2009 (42 isolates). Identification of E. coli ST131 clades and blaCTX-M were determined by multiplex PCR. Sequence types of non-ST131 isolates were determined by whole-genome sequencing. RESULTS: Although the prevalence of ST131 was comparable in 2020 (74.2%) and 2008-2009 (78.6%), the subclades differed during the two time periods (C1-nM27: 40.2% in 2008-2009 vs. 78.8% in 2020; C1-M27: 32.1% in 2008-2009 vs. 9.1% in 2020). The incidence of blaCTX-M among ST131 isolates increased from 27.3% in 2008-2009 to 64.3% in 2020. blaCTX-M was found in 80.6% and 93.8% of C1-M27 and C2 in 2020, respectively, and blaCTX-M possession in C1-nM27 increased from 19.2% in 2008-2009 to 40% in 2020. FQ-resistant ST1193 was detected only in 2020 (17.9% of 151 isolates, of which 14.8% possessed blaCTX-M). CONCLUSION: Increased resistance of E. coli to FQs and third-generation cephalosporins in Japan can be attributed to the accumulation of blaCTX-M in C1-nM27 and the increase of C1-M27 and C2 clades with high blaCTX-M possession, alongside the spread of ST1193.
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Proteínas de Escherichia coli , beta-Lactamases , Antibacterianos/farmacologia , Escherichia coli/genética , Proteínas de Escherichia coli/genética , Fluoroquinolonas/farmacologia , Japão/epidemiologia , beta-Lactamases/genéticaRESUMO
BACKGROUND: Gold nanoparticles are promising nanomaterials for catalytic reactions, sensing/imaging systems, photonic/plasmonic devices, and electronics because of their unique physical and chemical properties. To date, significant catalytic activities of gold nanoparticles have been reported for reactions such as carbon monooxide oxidation and 4-nitrophenol reduction, and diverse gold nanoparticle morphologies such as nanospheres, wires, rods, and cubes have been achieved using a variety of capping/stabilizing organic molecules. However, there are few reports on the simultaneous assembly of peptides forming secondary structures and metallic nanoparticles into peptide-metallic particle hybrids under mild aqueous conditions and demonstration of their use as catalysts. Furthermore, the gold nanoribbon surfaces are covered with ß-sheet structures, disrupting the access of substrates to the active sites, thereby possibly inhibiting their catalytic activity. OBJECTIVES: The main objective of this study is design, synthesis, and characterization of peptidegold nanoparticle hybrids that are prepared by an α-helical conformation of a template and examination of the catalytic activities of the hybrids. METHODS: We here report (i) the design, synthesis, and characterization of a new template peptide, RU025, that tends to form an α-helical conformation and self-assembles into network nanoarchitectures in aqueous solution through possibly hydrophobic and electrostatic interactions, (ii) the characterization of gold seed crystals synthesized by mixing RU025 and HAuCl4, (iii) the characterization of peptide-gold nanoparticle hybrids directed by crystal growth with NaBH4 and the dependence on the conditions used for nucleation, and (iv) the catalytic activities of the hybrids towards the reduction of 4-nitrophenol to 4-aminophenol in the presence of excess NaBH4. RESULTS: We demonstrated the design, synthesis, and characterization of a new template peptide, RU025, that tends to form an α-helical conformation and self-assembles into network nanoarchitectures in aqueous solution. Gold seed crystals were synthesized by mixing RU025 and HAuCl4 in a 1:2 molar ratio, followed by further reduction of the gold seed crystals with NaBH4. This reaction afforded worm-like gold nanoparticles embedded in the peptide self-assemblies. The peptide-gold nanoparticle hybrids exhibited catalytic activities for the Langmuir-Hinshelwood type reduction of 4-nitrophenol to 4-aminophenol in the presence of excess NaBH4, with an activation energy of 33 kJ mol-1. CONCLUSION: The size and morphology of gold nanoparticles can be tuned in the nanometer range by altering the peptide concentration relative to HAuCl4 and by changing the nucleation time. This method for constructing peptide-metallic nanoparticle hybrids, in which metallic nanoparticles are dispersed in the peptide self-assemblies, provides highly reactive catalysts.
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Ouro/química , Nanopartículas Metálicas/química , Peptídeos/química , Catálise , Cloretos/química , Cristalização/métodos , Compostos de Ouro/química , Interações Hidrofóbicas e Hidrofílicas , Nanoconjugados/química , Tamanho da Partícula , Conformação Proteica em alfa-Hélice , Propriedades de SuperfícieRESUMO
BACKGROUND: Multidrug-resistant Escherichia coli, especially a lineage of O25b:H4-ST131, has increased and spread worldwide. The surveillance of cross-resistance of E. coli is necessary. METHODS: Cross-resistance to fluoroquinolones (FQs) and aminoglycosides (AGs) was examined in E. coli isolated in Hokkaido Prefecture, Japan, between 2008 and 2009. RESULTS: Gentamicin (GEN) resistance was more common in FQ-resistant isolates (30/112 strains; 26.8%) than in FQ-susceptible isolates (2/100 strains; 2%). The frequency of GEN resistance was similar in two groups of FQ-resistant strains, O25b:H4-ST131 genotype (22/87 strains; 25.3%) and a group of other FQ-resistant genotypes (8/25 strains; 32.0%). The main AG resistance gene was aac(3)-II (87.5% of GEN-resistant strains). The only amikacin-resistant strain which was FQ resistant carried the aac(6')-Ib-cr gene. CTX-M type extended-spectrum ß-lactamase (ESBL) genes were also found in FQ-resistant strains at a high frequency. However, the number of strains with both ESBL and AG-modifying enzyme genes was relatively low (8 strains). CONCLUSION: All FQ-resistant strains, not only O25b:H4-ST131, appeared to preferentially acquire ESBL genes and/or genes encoding AG-modifying enzymes; however, the acquisitions of these genes seemed to occur independently.
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Antibacterianos/farmacologia , Farmacorresistência Bacteriana Múltipla/genética , Infecções por Escherichia coli/tratamento farmacológico , Escherichia coli/efeitos dos fármacos , Aminoglicosídeos/farmacologia , Escherichia coli/isolamento & purificação , Fluoroquinolonas/farmacologia , Humanos , Japão , Testes de Sensibilidade Microbiana , PrevalênciaRESUMO
We identified and genetically characterized seven fluoroquinolone-resistant Streptococcus pneumoniae strains among 293 clinical strains isolated from 1999 to 2001 in Japan. The resistant strains were isolated only from adults, and 7 of 31 isolates (22.6%) were from patients more than 20 years old. Resistant strains were not found in 262 isolates from children under age 10.