[Health care standards for surgical patients in clinical hospital]. / O standartakh meditsinskoi pomoshchi khirurgicheskim bol'nym v klinicheskom statsionare.

In Russian Federation the Obligatory Health Insurance Standards are based on the a priori expert assessments but not on statistical analysis of really recorded cases. This leads to the essential standard incompleteness, indistinctness and heterogeneity. In Vishnevsky Institute of surgery the "Automatized system for care registration in hospital" was designed, and data base on more than 10,000 patients treated in 1995 to 1999 was created giving now the starting-point for further efforts for overcoming the above standards insufficiency.

[The effect of second messengers on neutrophil movement]. / Vliiane vtorichnykh posrednikov na dvigatel'nuiu aktivnost' neitrofilov.

Effects of cAMP and cGMP dibutyryl derivatives (dbt), calcium ionophore A23187 and the protein kinase C activator PMA phorbol ester on mobility of human blood neutrophils were studied using the Magiskan 2A system of image processing. These drugs were added at concentrations, which allowed to produce similar inhibition of the cells mobility using a pattern of the middle velocity measurement in the cell population: dbt cAMP 5.10(-5)M, A23187 10(-6)M, PMA 10(-5)M; dbt cGMP 5.10(-6)M stimulated the neutrophil mobility. Differences in the patterns of mobility inhibition were detected by means of spectral characteristics related to alteration in the area of individual neutrophils. The drugs with inhibitory action (cAMP, A23187 and PMA) caused a decrease in the spectrum capacity of the area alteration as compared with normal state, while they affected dissimilarly the spectrum frequency characteristics. Dbt cAMP did not alter the frequency characteristics, A23187 shifted the spectrum towards a high frequency region and PMA--towards a low frequency range. Dbt cGMP did not alter the spectrum capacity but shifted it slightly to low frequency region. These alterations in the spectrum frequency characteristics are discussed considering the effect of secondary messengers on three types of the neutrophil movement which exhibit inherent frequencies.

[Role of the functional state of polymorphonuclear leukocytes in the pathogenesis of experimental sepsis]. / Rol' funktsional'nogo sostoianiia polimorfoiadernykh leikotsitov v patogeneze éksperimental'nogo sepsisa.

The functional states of the polynuclear leukocytes examined on the original experimental sepsis model have been studied. It has been shown that the bioactive factors of the microorganisms define the functional states of the polynuclear leukocytes. The features of the different forms of the sepsis are explained with such phenomenon.

[Neutrophil motility in patients with early infection (rapid analysis using automatic image processing system, Magiscan)]. / Podvizhnost' neitrofilov u bol'nykh s ranevoi infektsiei (ékspress-analiz na osnove sistemy obrabotki izobrazhenii Magiskan).

The authors studied the dependence of blood neutrophil motility on the physical state of patients with infected wounds. Neutrophil motility was recorded and analyzed with the help of Magiscan-2a system of automatic image processing. A special software was developed for monitoring the movement of cells and measuring their displacements. Motility of each separate cell and a mean motility of the neutrophil population were determined on the glass in the microscope field (512 x 512 microns). Velocity of each neutrophil was shown to be nonuniform but approximately constant. Cell velocities in each probe were distributed in a continuous manner. Mean velocity of neutrophils in the probe was a stable index of the population motility. Decrease in neutrophil motility in the blood of patients with infected wounds was probably due to the adverse effect of serum factors. The application of the system of image processing makes it possible to carry out express analysis of neutrophil motility to estimate the efficiency of medical treatment.

[Method of automatic recording and analysis of leukocyte migration based on the image analysis system]. / Metod avtomaticheskoi registratsii i analiza migratsii leikotsitov na baze sistemy obrabotki izobrazhenii.

The method of automatic registration and of the analysis of neutrophils' mobility with the use of the Image Analysis System is described in this paper. The authors developed an algorithm of cells identification which makes it possible to carry out cadre-by-cadre observing the shift of each cell's centre of gravity with the time interval between cadres more than 15 seconds. This algorithm also allows to measure areas, perimeters and orientation of cells and their changes. Looking after the shifts of the cells' centres of gravity allows to describe the cells' paths in the form of the following dependences: path-by-time, shift-by-time, angle of vector of shift alteration. Observing a great number of cells at the same time allows to get true statistical picture of neutrophils' activity and distribution of cells' activity in population of neutrophils during only some minutes of registration. It is shown that each cell in the population has its own constant velocity.