Children's experiences of care on walking and cycling journeys between home and school in Healthy New Towns: Reframing active school travel.

The Healthy New Town programme in England set out to 'put health into place' by supporting the design and construction of healthy places to live, including by creating safe environments for active travel. To explore the impact of this approach, this study examined how children and their families experienced school journeys in two contrasting Healthy New Towns in England, one an affluent new town in the early stages of construction and the other more economically deprived and established. We undertook photo-elicitation and go-along interviews with 24 children aged 7-12 years and semi-structured interviews with 17 caregivers. We found that experiences of care were important for children's school travel. In the 'deprived' town, opportunities for children to care and to be cared for were enjoyed, facilitated by routes with limited traffic, pockets of 'nature', and possibilities to encounter meaningful others. For families living in a town under construction, the need to negotiate unfinished travel infrastructure, and a sense of being 'in limbo', was experienced as an absence of care by planners and developers. Interventions to promote children's active travel should consider the role of care-full planning in facilitating walking and cycling journeys.

Doing more with movement: constituting healthy publics in movement volunteering programmes.

The recent phenomenon of movement volunteering programmes is a form of 'fitness philanthropy' that combines exercise with volunteering in order for physical activity to generate a more widely shared set of benefits. These newest practices of fitness philanthropy radically rework both exercise and volunteering through the ways in which these come together and take place outdoors and in the everyday spaces of the street or community. The paper explores these new practices through the movement volunteering programme 'GoodGym', in relation to the concept of 'healthy publics'. Fieldwork comprised ethnography, including participant observation, interviews, go-along interviews, conversations, photography and an end of fieldwork discussion workshop. We focus on the experiences of three different constituencies in GoodGym: the volunteers; the participants and passers-by; the space and atmosphere. The formation of these dynamic, multiple and shifting healthy publics emerge through the complex intersections of several processes. We draw particular attention to the centrality in the new fitness philanthropy practices of visibility and spectacle, sociality and merging mobilities in constituting healthy publics.

Estrogen mimetic 4-tert-octylphenol enhances IgE-mediated degranulation of RBL-2H3 mast cells.

Allergic diseases such as asthma have been on the rise in recent decades. Environmental or occupational exposure to estrogenic synthetic chemicals is suspected to be a contributing factor, and previous experimental studies indicated that estradiol and some xenoestrogens increase allergic signaling responses, such as degranulation, in immune cells. In the current study, data showed that the estrogen mimetic 4-tert-octylphenol (4tOP) enhances immunoglobulin (Ig) E-mediated degranulation of mammalian mast cell line RBL-2H3 (RBL). At the noncytotoxic concentrations 10-20 µM, 4tOP significantly increased degranulation in antigen (Ag)-activated RBLs but exerted no marked effect on spontaneous levels. Our data suggest that the industrial chemical 4tOP has the potential to enhance allergic disease in individuals who are exposed.

Antibacterial agent triclosan suppresses RBL-2H3 mast cell function.

Triclosan is a broad-spectrum antibacterial agent, which has been shown previously to alleviate human allergic skin disease. The purpose of this study was to investigate the hypothesis that the mechanism of this action of triclosan is, in part, due to effects on mast cell function. Mast cells play important roles in allergy, asthma, parasite defense, and carcinogenesis. In response to various stimuli, mast cells degranulate, releasing allergic mediators such as histamine. In order to investigate the potential anti-inflammatory effect of triclosan on mast cells, we monitored the level of degranulation in a mast cell model, rat basophilic leukemia cells, clone 2H3. Having functional homology to human mast cells, as well as a very well defined signaling pathway leading to degranulation, this cell line has been widely used to gain insight into mast-cell driven allergic disorders in humans. Using a fluorescent microplate assay, we determined that triclosan strongly dampened the release of granules from activated rat mast cells starting at 2 µM treatment, with dose-responsive suppression through 30 µM. These concentrations were found to be non-cytotoxic. The inhibition was found to persist when early signaling events (such as IgE receptor aggregation and tyrosine phosphorylation) were bypassed by using calcium ionophore stimulation, indicating that the target for triclosan in this pathway is likely downstream of the calcium signaling event. Triclosan also strongly suppressed F-actin remodeling and cell membrane ruffling, a physiological process that accompanies degranulation. Our finding that triclosan inhibits mast cell function may explain the clinical data mentioned above and supports the use of triclosan or a mechanistically similar compound as a topical treatment for allergic skin disease, such as eczema.