Assuntos
Produtos do Gene gag/metabolismo , Retroelementos/fisiologia , Retroviridae/metabolismo , Membrana Celular/metabolismo , Escherichia coli/genética , Produtos do Gene gag/biossíntese , Produtos do Gene gag/genética , Concentração de Íons de Hidrogênio , Íons/metabolismo , Substâncias Macromoleculares , Fosforilação , RNA/metabolismo , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Retroviridae/genética , Retroviridae/fisiologia , Montagem de Vírus/fisiologiaAssuntos
Escherichia coli/genética , Retroelementos , Fatores de Transcrição/genética , Sequência de Aminoácidos , Bacteriófago T7/genética , Sequência de Bases , Clonagem Molecular , DNA Recombinante , Eletroforese em Gel de Poliacrilamida , Dados de Sequência Molecular , Fases de Leitura Aberta , Proteínas Recombinantes/genéticaRESUMO
It is demonstrated by filter-binding assay that casein kinase 2 from Rana temporaria oocytes binds rRNA in vitro with high affinity. Ligand-blotting shows that rRNA-binding activity is inherent to alpha and alpha' subunits of the enzyme. Increase of pH from 6.5 to 7.5 has little effect on casein kinase but completely suppresses rRNA-binding activity of the enzyme. Sedimentation coefficient of casein kinase 2 also depends on pH: at pH 7.5 it is mainly 10 S, and at pH 6.5-18 S. At pH 6.95 the amounts of both forms are equal. The heavy form of casein kinase 2 practically lacks rRNA-binding activity.
Assuntos
Oócitos/enzimologia , Proteínas Quinases/metabolismo , RNA Ribossômico/metabolismo , Animais , Caseína Quinases , Feminino , Concentração de Íons de Hidrogênio , Cinética , Substâncias Macromoleculares , Peso Molecular , Ligação Proteica , Proteínas Quinases/isolamento & purificação , Rana temporariaRESUMO
The variability of Q-heterochromatin regions (Q-HR) was studied in native residents of the northern part of West Siberia, viz Yakuts (n = 127), Selkups (n = 90) and Khants (n = 54), as well as in newcomers including oil-borers (n = 43) and children (n = 113) living permanently in this part of the USSR. The major quantitative characteristics of chromosomal Q-HR variability were shown to be very similar in oil-borers and natives, and this is considered to be the result of specific selection of individuals according to the amount of Q-HRs in their genome. The hypothesis on the possible selective value of chromosomal Q-HRs in human adaptation to extreme environmental conditions of the extreme north is discussed.