Longitudinal Survey on aMPV Circulation in French Broiler Flocks following Different Vaccination Strategies.

In recent years, the impact of respiratory disease resulting from Avian Metapneumovirus (aMPV) infection has been generally rising in the broiler industry in Europe. In this context, in order to investigate aMPV contribution to the clinical picture and the potential benefits of diversified vaccination strategies compared to nonvaccination policies, a longitudinal monitoring was performed, also evaluating Infectious Bronchitis Virus (IBV) presence. Broiler flocks located in Western France, where aMPV has already proven to be a health and productivity issue, were screened by RT-PCR on rhino-pharyngeal swabs, and the viruses were genetically characterized by sequence analysis. For a more comprehensive picture of aMPV molecular epidemiology and evolution in France, aMPV subtype B strains detected from 1985 to 1998 were sequenced and included in the analysis. The survey confirmed the detection of aMPV subtype B in commercial broiler flocks in France, together with a certain heterogeneity demonstrated by the circulation of more recent and historical French field strains. No IBV field strains were detected. The implementation and evaluation of different management choices and vaccine strategies suggests once again that immunization does not prevent infection but contributes greatly to the containment of the clinical manifestations.

What is new on molecular characteristics of Avian metapneumovirus strains circulating in Europe?

In the present study, one hundred and sixteen partial G gene sequences of Avian metapneumovirus (aMPV) subtype B, obtained during routine diagnostics in different European Countries in the last few years (2014-2019), were analysed by sequence and phylogenetic analyses in order to draw an updated picture of the molecular characteristics of circulating strains. Nucleotide sequences were compared with other sequences of European and non-European aMPV-Bs collected prior to that period or retrieved from GenBank. Phylogenetic relationships among the aMPV-B strains, reconstructed using the maximum likelihood method implemented in MEGA X, demonstrated that aMPV-B has evolved in Europe from its first appearance, frequently displaying a clear relation with the geographic area of detection. The 40% of aMPV-B viruses analysed were classified as vaccine-derived strains, being phylogenetically related, and showing high nucleotide identity with live commercial vaccine strains licensed in Europe. The remaining 60% were classified as field strains since they clustered separately and showed a low nucleotide identity with vaccines and vaccine-derived strains. The phylogenetic tree showed that the virus has continued to evolve from its first appearance in the '80s since more recently detected strains belonged to clades phylogenetically distant from the older strains. Unlike vaccine-derived strains, field strains tended to cluster according to their geographic origin and irrespective of the host species where the viruses had been detected. In conclusion, the molecular characterization of aMPV-B and the differentiation between vaccines and field strains through G gene sequence analysis can be a useful tool towards correct diagnosis and should be routinely applied in order to better address the control strategies.

Detection of Histomonas meleagridis DNA in dust samples obtained from apparently healthy meat turkey flocks without effect on performance.

Environmental dust samples obtained from 65 turkey flocks in France, of which six suffered from histomonosis whereas the rest remained apparent healthy until the end of production, were tested for the presence of Histomonas meleagridis DNA by recently developed real-time PCR based on the 18S rRNA locus. In order to determine the genotype of detected histomonads, positive samples were further subjected to conventional 18S PCR and sequencing. Additionally, production data of all tested flocks, such as average daily gain, feed conversion ratio and production index, were statistically evaluated and compared to see the effect of positive dust samples in apparently healthy flocks. Histomonad DNA was detected in the dust obtained from all six clinically affected and, surprisingly, in nine apparent healthy flocks. Sequencing of the 18S rRNA gene resulted in only one DNA sample homologous to H. meleagridis whereas 11 others revealed the presence of several other flagellates. Average daily gain and production index were negatively affected in flocks with clinical histomonosis, resulting in significant difference in comparison with the data obtained from clinically healthy flocks independent of the presence of histomonad DNA in the dust. Overall, there was no significant difference following statistical analysis of production parameters between the two last mentioned groups of tested flocks. Altogether, this is the first investigation demonstrating the presence of H. meleagridis DNA in environmental dust samples obtained from clinically unaffected turkey flocks. However, this finding could not be correlated with impact on production based on analysis and comparison of selected production data. RESEARCH HIGHLIGHTS Environmental dust obtained from clinically healthy turkey flocks, in addition to dust from flocks affected by histomonosis, was found positive for the presence of Histomonas meleagridis DNA. Histomonas-positive dust samples in clinically unaffected flocks did not have a negative effect on production parameters. The results demonstrate a wider spread of H. meleagridis DNA in flocks of commercial meat turkeys than previously thought.