RESUMO
Plumbagin is the main pharmacologically active compound of carnivorous plants in the genera Drosera. It possesses various pharmacological activities, including anticancer and antimalarial activities, and is used in traditional medicine. In this study, we reported a sustainable production system of plumbagin by adding sodium acetate and L-alanine as precursors to in vitro cultures of Drosera burmannii Vahl and Drosera indica L. In addition, plumbagin production was reported in the cultures subjected to different color LED lights. The highest plumbagin level (aerial part 14.625⯱â¯1.007â¯mg·g-1 DW and root part 1.806⯱â¯0.258â¯mg·g-1 DW) was observed in D. indica cultured under blue LED light for 14â¯days, and further culturing did not increase plumbagin production. In addition, plumbagin enhancement by precursor feeding (9.850⯱â¯0.250â¯mg·g-1 DW, 1.2-fold) was observed in the aerial part of D. indica treated with 50â¯mg·L-1 sodium acetate for 3â¯days. Comparing both plants, up to 700-fold higher plumbagin was observed in D. indica than in D. burmannii. Moreover, in both plants, the aerial part accumulated higher plumbagin (up to 10-fold) than the roots. This is the first report on the effect of artificial LED lights on the plumbagin level of Dorsera plants. The culturing of D. indica under blue LED light showed enhanced plumbagin levels and suggests a fast and simple system for the in vitro production of plumbagin.