RESUMO
A 1-month-old rabbit, imported as a pet by a distributor, died suddenly in the quarantine period in Japan due to suppurative pleuropneumonia. A bacterial isolate from its right lung was identified as Pasteurella multocida serotype A: 11. The isolate was classified as ST204 using the RIRDC scheme of multilocus sequence typing, suggesting that the isolate was genetically related to European isolates of the same sequence type listed in the PubMLST database and not to four other isolates that originated from past imported rabbits. In the immunohistochemical assay, an antiserum recognizing the somatic serotype 11 antigen generated from chicken could specifically detect P. multocida, indicating that the antiserum for somatic serotyping was useful for immunohistochemical diagnosis of rabbit pasteurellosis.
Assuntos
Infecções por Pasteurella/veterinária , Pasteurella multocida/isolamento & purificação , Coelhos/microbiologia , Animais , Anticorpos , Galinhas , Tipagem de Sequências Multilocus/métodos , Tipagem de Sequências Multilocus/veterinária , Infecções por Pasteurella/epidemiologia , Infecções por Pasteurella/patologia , Pasteurella multocida/genética , Pasteurella multocida/patogenicidade , Pleuropneumonia/microbiologia , Pleuropneumonia/patologia , Pleuropneumonia/veterinária , Sorotipagem/veterinária , Taiwan/epidemiologiaRESUMO
OBJECTIVES: This study aimed to determine the prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in imported swine during the quarantine period in Japan. METHODS: Nasal swabs from a total of 125 swine belonging to 15 lots (unit of import) from five countries were investigated for MRSA from July 2016 to February 2017. Two isolates per positive lot were chosen for multilocus sequence typing (MLST). PCR was performed to determine the presence of the czrC gene, and antimicrobial susceptibility testing was performed by the broth dilution method. RESULTS: MRSA isolates were obtained from six lots (41 heads; 32.8%) from two countries. All 12 isolates that underwent MLST (two per positive lot) were classified as ST398, harboured the czrC gene and were resistant to ampicillin and tetracycline; some isolates showed additional resistance to erythromycin or streptomycin, but resistance to ciprofloxacin, gentamicin or chloramphenicol was not observed. CONCLUSIONS: MRSA ST398 isolates were obtained from imported swine in this first trial to monitor MRSA during the quarantine period in Japan. For the 'One Health' approach against antimicrobial resistance, monitoring imported animals and generating feedback data would be important.
Assuntos
Doenças Transmissíveis Importadas/veterinária , Staphylococcus aureus Resistente à Meticilina/genética , Staphylococcus aureus Resistente à Meticilina/isolamento & purificação , Quarentena/veterinária , Infecções Estafilocócicas/veterinária , Doenças dos Suínos/epidemiologia , Doenças dos Suínos/prevenção & controle , Animais , Antibacterianos/farmacologia , Técnicas de Tipagem Bacteriana , Doenças Transmissíveis Importadas/epidemiologia , Doenças Transmissíveis Importadas/prevenção & controle , Farmacorresistência Bacteriana Múltipla/genética , Eletroforese em Gel de Campo Pulsado , Japão , Meticilina/farmacologia , Staphylococcus aureus Resistente à Meticilina/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Tipagem de Sequências Multilocus , Saúde Única , Reação em Cadeia da Polimerase , Prevalência , Infecções Estafilocócicas/epidemiologia , Infecções Estafilocócicas/prevenção & controle , Suínos , Doenças dos Suínos/microbiologiaRESUMO
An imported crossbred Angus beef steer aged eight to twelve months died suddenly on the eighth day of a quarantine period in Japan. Gross examination showed the peritoneum and mesentery consisted of numerous nodules of various sizes. Histological examination revealed chronic suppurative granulomatous peritonitis with eosinophilic rosettes surrounding colonies of Gram-negative bacilli. The bacteria isolated from the nodules were confirmed to be Actinobacillus lignieresii based on the results of 16S rRNA gene sequencing and immunohistochemistry. Antibiotic sensitivity testing showed that the isolate was resistant to penicillin. Thus, a diagnosis of atypical actinobacillosis caused by A. lignieresii was made.