1.
Biotecnol. apl
; 8(2): 123-39, mayo-ago. 1991. ilus, tab
Artigo
em Espanhol
| LILACS
| ID: lil-111947
2.
Biotecnol. apl
; 8(2): 123-39, mayo-ago. 1991. ilus, tab
Artigo
em Espanhol
| CUMED
| ID: cum-8422
3.
Theriogenology
; 35(3): 625-32, 1991 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-16726931
RESUMO
We report here on the microinjection of the human growth hormone gene (hGH) into the germinal disc of common carp one-cell embryos. For microinjection, a linear 4.1 kb EcoRi-DNA fragment containing the mouse metallothionein-I promoter (mMT-I), fused to a structural gene coding for the hGH, was used. Of the 14 injected embryos, 9 developed to an age of 60 d. Of these nine fish, two were found to be transgenic and had integrated two to four copies per cell of the hGH construct.