Determination of in vitro dry matter, protein, and fiber digestibility and fermentability of novel corn coproducts for swine and ruminants.

New processes are being used in some dry-grind ethanol plants in the United States and Brazil to improve ethanol yield and efficiency of production while also providing nutritionally enhanced corn coproducts compared with conventional corn distillers dried grains with solubles (DDGS). The objectives of this study were to determine the chemical composition and in vitro digestibility of 5 conventional corn DDGS sources and 10 emerging novel corn coproducts for swine and ruminants, and compare coproducts produced using similar processes in the United States and Brazil. Chemical composition, on a dry matter (DM) basis, among the 15 coproducts ranged from 18.5% to 54.7% for crude protein (CP), 12.3% to 51.4% for neutral detergent fiber (NDF), 1.6% to 8.6% for acid detergent fiber, 4.7% to 12.3% for ether extract, and 1.6% to 8.6% for ash. For swine, in vitro hydrolysis of DM and CP were greater (P < 0.01) for the three U.S. corn DDGS sources compared with the two Brazilian corn DDGS sources, but in vitro fermentability of DM was comparable (P > 0.05) among all sources except one U.S. DDGS source that had less fermentable DM. High-protein and yeast dried distillers grains (Ultramax, UM; StillPro, SP) coproducts also had comparable (P > 0.05) DM fermentability for swine, but UM coproducts had greater (P < 0.01) DM and CP hydrolysis compared with SP. High-protein distillers dried grains (HP-DDG) from Brazil had greater (P < 0.01) DM and CP hydrolysis, but less (P < 0.01) DM fermentability for swine than HP-DDG produced in the United States, using the same process. For ruminants, total DM digestibility was greater (P < 0.01) in conventional DDGS sources from the United States compared with the two DDGS sources from Brazil. Total protein digestibility for ruminants was comparable and above 81% for all coproducts except for a DDGS source from Brazil, a HP-DDG source from the United States, and a UM sample. Interestingly, the corn fiber + solubles coproduct had not only relatively high digestibility of NDF (67.9%), DM (91.6%), and total CP (81.9%) for ruminants, but it also had relatively high total tract digestibility of DM (86.2%) and CP (69.9%) for swine. These results suggest that nutrient digestibility of conventional DDGS sources produced in the United States appear to be greater than corn Brazilian DDGS sources, but new process technologies being implemented in ethanol and coproduct production in both countries can enhance the nutritional value of corn coproducts for both swine and ruminants.

A meta-analysis to predict the concentration of standardized ileal digestible amino acids in distillers dried grains with solubles for poultry.

High variability in amino acid (AA) content and digestibility among sources of distillers dried grains with solubles (DDGS) create challenges for nutritionists when using it in precision nutrition feeding programs. The objective of this meta-analysis was to develop prediction equations for standardized ileal digestible content (SIDC) of AA in DDGS for poultry. A meta-analysis based on 86 observations from 19 publications was conducted to develop equations for predicting SIDC of AA based on chemical composition in DDGS. A mixed model was used to develop prediction equations for SIDC of AA, and a backward selection of variables was conducted based on chemical composition of DDGS. Each total AA content was the best predictor of SIDC for all indispensable AA. The prediction equations for SIDC of Met and Lys were y = -0.12 + 1.05 × Met (R2 = 0.899) and y = -0.22 + 0.91 × Lys (R2 = 0.870), respectively. The prediction equations for SIDC of Met and Lys using data from broiler chick assays were y = -0.16 + 1.12 × Met (R2 = 0.809) and y = -0.24 + 0.90 × Lys (R2 = 0.731), respectively, and equations derived from rooster assay data for Met and Lys were y = -0.05 + 0.97 × Met (R2 = 0.996) and y = -0.20 + 0.97 × Lys (R2 = 0.982), respectively. For all the prediction equations, the intercept (=0) and slope (=1) were not different (P > 0.10) between predicted and observed SID AA values, indicating high accuracy of the models. In conclusion, equations for predicting SIDC of AA in DDGS were developed and showed that total concentration of each AA was the best predictor of SDIC. These prediction equations can be used by animal nutritionists to more accurately estimate SIDC of AA among DDGS sources in diet formulations for precision poultry nutrition feeding programs.

Identification of redox imbalance as a prominent metabolic response elicited by rapeseed feeding in swine metabolome.

Rapeseed (RS) is an abundant and inexpensive source of energy and AA in diets for monogastrics and a sustainable alternative to soybean meal. It also contains diverse bioactive phytochemicals that could have antinutritional effects at high dose. When the RS-derived feed ingredients (RSF) are used in swine diets, the uptake of these nutrients and phytochemicals is expected to affect the metabolic system. In this study, 2 groups of young pigs (17.8 ± 2.7 kg initial BW) were equally fed a soybean meal-based control diet and an RSF-based diet, respectively, for 3 wk. Digesta, liver, and serum samples from these pigs were examined by liquid chromatography-mass spectrometry-based metabolomic analysis to determine the metabolic effects of the 2 diets. Analyses of digesta samples revealed that sinapine, sinapic acid, and gluconapin were robust exposure markers of RS. The distribution of free AA along the intestine of RSF pigs was consistent with the reduced apparent ileal digestibility of AA observed in these pigs. Despite its higher fiber content, the RSF diet did not affect microbial metabolites in the digesta, including short-chain fatty acids and secondary bile acids. Analyses of the liver and serum samples revealed that RSF altered the levels of AA metabolites involved in the urea cycle and 1-carbon metabolism. More importantly, RSF increased the levels of multiple oxidized metabolites and aldehydes while decreased the levels of ascorbic acid and docosahexaenoic acid-containing lipids in the liver and serum, suggesting that RSF could disrupt redox balance in young pigs. Overall, the results indicated that RSF elicited diverse metabolic events in young pigs through its influences on nutrient and antioxidant metabolism, which might affect the performance and health in long-term feeding and also provide the venues for nutritional and processing interventions to improve the utilization of RSF in pigs.

Prediction of digestible and metabolizable energy of corn distillers dried grains with solubles for growing pigs using in vitro digestible nutrients.

The objective of this study was to develop prediction equations (EQS) that estimate DE and ME content of corn distillers dried grains with solubles (DDGS) using digestible (DIG) nutrients from in vitro or in vivo assays. Chemical composition data from 12 sources of corn DDGS were obtained using laboratory chemical analysis (LCA) and near infrared spectroscopy (NIRS). In vitro DM disappearance (IVDMD) from gastric and small intestine hydrolysis (IVDMDh), large intestine fermentation (IVDMDf), and total tract digestion (IVDMDt) were also determined along with in vivo apparent total tract digestibility (ATTD) of DM, CP, ether extract (EE), NDF, and ADF, and energy values (GE, DE, and ME). Correlation analysis was used to compare chemical composition from LCA with NIRS, and a stepwise selection of variables was performed using linear regression to establish DE and ME prediction EQS. Composition determined by NIRS did not correlate with values from LCA. Consequently, significance and R2 were poorer when using NIRS data as inputs to predict DE (P = 0.11; R2 = 0.23) and ME (P = 0.11; R2 = 0.24). However, when using LCA data, DE (P = 0.04; R2 = 0.35) and ME (P = 0.04; R2 = 0.52) estimates of corn DDGS obtained from prediction EQS had significant P values, but low R2. Better prediction of DE (P < 0.01; R2 = 0.83) and ME (P < 0.01; R2 = 0.76) was observed when using in vivo DIG nutrients as inputs (DM basis) for DE, kcal/kg = 854.5 + (3.6 × DIG DM, g/kg) + (3.7 × DIG EE, g/kg) + (2.0 × DIG NDF, g/kg) and ME, kcal/kg = 704.5 + (3.3 × DIG DM, g/kg) + (4.8 × DIG EE, g/kg) + (2.6 × DIG NDF, g/kg). In vitro DIG DM, but not in vitro DIG NDF, was selected for use in EQS (7) DE, kcal/kg = 6,383.6 - (42.5 × in vitro DIG DM, g/kg) + (35.4 × DIG EE, g/kg) and (8) ME, kcal/kg = 6,635.1 - (49.8 × in vitro DIG DM, g/kg) + (41.3 × DIG EE, g/kg), but the significance and accuracy for both DE (P = 0.07; R2 = 0.45) and ME (P = 0.05; R2 = 0.49) predictions was less using in vitro DIG nutrient values than using in vivo DIG nutrient values. If chemical composition was used to replace in vivo EE, along with in vitro DIG NDF and DM, the prediction EQS only used the concentration of ADF and EE as predictors instead of in vitro DIG NDF and DM. In conclusion, in vivo DIG NDF, DM, and EE are the best predictors for DE and ME content of corn DDGS for swine. Using NIRS to determine chemical composition, in vitro DIG NDF, and in vitro DIG DM did not result in accurate predictions of DE and ME.

Nutrient composition, digestible and metabolizable energy content, and prediction of energy for animal protein byproducts in finishing pig diets.

An industry survey and animal experiment were conducted to evaluate compositional variability and DE and ME content of animal protein byproducts and to generate equations to predict DE and ME content based on chemical analysis. For the 220 samples collected, the greatest concentration of CP was observed in blood meal (BM) and the least in meat and bone meal (MBM) and the greatest concentration of ether extract was in meat meal and the least in BM, with ash content greatest in MBM and least in BM, with Ca and P levels being 36.1 and 16.3% of the ash content, respectively. For the balance experiment, a corn-soybean meal basal diet was used with test diets formulated by mixing 80% of the basal diet with 20% of the animal protein byproduct, except for BM, which was included at 10 and 20% of the test diets. Ten groups of 24 gilts (92.5 ± 7.4 kg final BW) were randomly assigned to the test or basal diet within each group, resulting in 16 replications per animal protein byproduct or basal diet, except for BM determinations (20 replications). Gilts were placed in metabolism crates and offered 2.4 kg daily of their assigned diet for 13 d, with total collection of feces and urine during the last 4 d. Gross energy in the diets, feces, and urine was used to calculate the DE and ME content of each ingredient by the difference procedure, using DE and ME of the basal diet as a covariate among groups of pigs. The DE content of the animal protein byproducts ranged from 5,367 to 2,567 kcal DE/kg DM, and ME ranged from 4,783 to 2,340 kcal ME/kg DM. Using all animal protein byproducts, the best-fit equations were as follows: DE (kcal/kg DM) = -2,468 + (1.26 × GE, kcal/kg DM), with of 0.84, SE = 390, and < 0.01, and ME (kcal/kg DM) = -2,331 + (1.15 × GE, kcal/kg DM), with of 0.86, SE = 327, and < 0.01. The apparent total tract digestibility (ATTD) of Ca and P were also determined using the difference procedure, with the average ATTD of Ca and P for the animal protein byproducts, excluding BM and feather meal, being 27.1 and 39.1%, respectively. These data indicate that DE and ME substantially varied among the animal protein byproducts and sources and that a variety of nutritional components can be used to accurately predict DE and ME for finishing pigs. In addition, it appears that high dietary inclusion rates of animal protein byproducts may result in low ATTD estimates of Ca and P, which may be due to excessive concentrations of total Ca and P affecting digestibility.

Effect of dietary fiber and diet particle size on nutrient digestibility and gastrointestinal secretory function in growing pigs.

Reduction of diet particle size (PS) increases feed efficiency due to an increase in the apparent total tract (ATTD) of GE. However, other effects of PS on the gut secretory function are not known. Therefore, the objective of this experiment was to measure the effect of diet composition (DC) and PS on nutrient digestibility, gastrointestinal hormones, total bile acids (TBA), total cholesterol and glucose concentrations in plasma of finishing pigs ( = 8/diet). Pigs were fed finely (374 ± 29 µm) or coarsely (631 ± 35 µm) ground corn-soybean meal (CSB), CSB + 35% corn dried distillers' grains with solubles (DDGS), and CSB with 21% soybean hulls (SBH) diets for 49 d. Diet composition, nutrient digestibility, along with fasting plasma concentrations of gastrin, insulin, glucagon-like peptide-1 (GLP-1), glucose-dependent insulinotropic peptide (GIP), TBA, cholesterol, and glucose were measured. Fine ground diets had greater ( < 0.05) ATTD of GE as well as greater ( < 0.05) ME than coarse ground diets independent on the DC. Fine ground diets also had greater ( < 0.05) ATTD of DM, N, ether extract, and NDF, independent of DC. A decrease in PS also caused an increase ( < 0.05) in ATTD of N, K, and S, but it did not affect ATTD of Ca, P, or Na. The DC and PS affected plasma gastrin, insulin and TBA but not GIP, GLP-1, glucose, and cholesterol. Gastrin concentration was greater ( < 0.05) in pigs fed coarse DDGS compared with feeding coarse CSB and SBH diets. Insulin concentration of pigs fed CSB was greater ( < 0.01) in pigs fed fine compared with coarse DDGS, and was greater ( < 0.05) in coarse compared with fine SBH diets. Pigs fed DDGS had greater ( < 0.05) TBA than those fed SBH and fine CSB diets. Gastrin, insulin, TBA and cholesterol tended ( < 0.10), or correlated ( < 0.05) with P, K and Fe intake. Insulin, TBA, and cholesterol were correlated ( < 0.05) with Na and S intake. In conclusion, a decrease in diet PS increases the ATTD of nutrients independently of DC, while mineral intake affects gastrointestinal secretion of hormones with potential metabolic impacts. Plasma insulin and glucose concentrations were correlated with DM intake, and glucose was associated with lipid and protein intake. Diet energy, nutrient digestibility, and plasma gastrin, insulin and TBA concentrations were affected by DC and PS.

Ultraviolet irradiation of spray-dried porcine plasma does not affect the growth performance of nursery pigs when compared with nonirradiated bovine plasma.

Ultraviolet light irradiation of spray-dried porcine plasma (SDPP) decreases the risk of disease transmission, but it may decrease the activity of bioactive components in SDPP. Therefore, the objectives of this study were to determine growth performance, morbidity, and mortality responses of nursery pigs fed UV-irradiated SDPP (UV-SDPP) compared with nonirradiated spray-dried bovine plasma (SDBP). Pigs ( = 480; 6.09 ± 2.4 kg initial BW) were blocked by initial BW, and blocks were assigned to pens. the sex ratio was equalized within blocks and pens. Pens were randomly assigned to 1 of 5 dietary treatments (8 pigs/pen and 12 replicates/treatment) in a 3-phase feeding program (phase 1 = d 0 to 13, phase 2 = d 14 to 27, and phase 3 = d 28 to 55). Dietary treatments included a control diet without UV-SDPP or SDBP and diets containing 3% UV-SDPP, 3% SDBP, 6% UV-SDPP, or 6% SDBP during phase 1. Diets were formulated to meet or exceed nutrient requirements and contained the same concentrations of standardized ileal digestible Lys and Lys:ME ratio within phases. Pigs were provided ad libitum access to diets throughout the 55-d experiment. Dietary inclusion rates during phase 2 were reduced to 1.5% UV-SDPP, 1.5% SDBP, 3% UV-SDPP, and 3% SDBP, and all pigs were fed a common diet without UV-SDPP or SDBP during phase 3. Growth performance data were analyzed as a 2 × 2 factorial arrangement of treatments with a control within a completely randomized block design to evaluate the main effects of plasma processing (UV irradiated vs. nonirradiated) and dietary inclusion level, and block, room, and pen were random effects. In phase 1, there were no differences in G:F among treatments, but pigs fed 6% UV-SDPP and 6% SDBP had greater ( < 0.01) ADG (0.11 vs. 0.08 kg/d) and ADFI (0.17 vs. 0.15 kg/d) than pigs fed the control, 3% SDBP, and 3% UV-SDPP diets. After phase 1 (d13), feeding UV-SDPP or SDBP increased ( = 0.02) the BW of pigs. In phases 2 and 3 and the overall feeding period (d 0 to 55), there were no differences in ADG, ADFI, and G:F among dietary treatments. There was a linear decrease ( < 0.01) in mortality of nursery pigs as dietary inclusion rate of SDBP and UV-SDPP increased. In conclusion, feeding SDBP or UV-SDPP diets improved ADG and ADFI during the first 2 wk after weaning due to improved feed consumption, and UV irradiation appeared to have no detrimental effects on the feeding value of SDPP.

Effects of adding minimally refined cottonseed oil or crude glycerol to diets containing 40% corn distiller's dried grains with solubles on growth performance, carcass characteristics, and pork fat firmness of growing-finishing pigs.

Diets containing more than 20% distiller's dried grains with solubles (DDGS) reduce fat firmness in pork, but supplementation of cottonseed oil or crude glycerol may improve fat firmness. The objective of this study was to assess the effect of feeding minimally refined cottonseed oil or crude glycerol on growth performance, carcass composition, and fat quality of growing-finishing pigs. Mixed sex pigs ( = 216; 24 ± 4 kg initial BW) were blocked by BW and allotted to 1 of 3 dietary treatments: 1) a basal corn-soybean meal diet with 40% DDGS (CON), 2) CON diet plus 5% minimally refined cottonseed oil added throughout the experiment (COT), or 3) CON fed during the first 8 wk and CON + 8% crude glycerol fed during the last 6 wk of the experiment (GLY). Although diets were not isocaloric, total AA-to-ME ratios were calculated to be equal among diets. Carcass composition was estimated using real-time ultrasound 2 d before harvest. Gilts (16/treatment) closest to the mean BW of each pen were harvested (115 ± 8 kg BW), and bellies were retrieved for in-depth analysis of fat quality. Belly fat was sampled and analyzed for fatty acid composition. Overall, ADFI of pigs fed COT (2.30 kg/d) was less ( < 0.01) than that of pigs fed CON or GLY (2.47 and 2.49 kg/d, respectively). Pigs fed COT (0.93 kg/d) had greater ( < 0.01) ADG compared with pigs fed CON or GLY (0.88 and 0.87 kg/d, respectively). Greater ( < 0.01) G:F was observed for pigs fed COT (0.41) than for pigs fed CON or GLY diets (0.36 and 0.35, respectively). Final BW of pigs fed COT (124.3 kg) was greater ( < 0.01) than that of pigs fed CON or GLY (118.9 and 118.6 kg, respectively). Pigs fed COT had greater ( < 0.01) HCW (94.9 kg) compared with pigs fed CON or GLY (89.9 and 89.2 kg, respectively). No differences were observed for dressing percentage (75.7, 76.3, and 75.3%), fat-free carcass lean percentage (50.5, 49.7, and 50.0%), and belly flop angle (6.21, 8.57, and 6.06°) for CON, COT, and GLY, respectively. Pigs assigned to COT had higher ( < 0.01) melting point of belly fat compared with pigs assigned to CON or GLY (30.4 vs. 26.3 and 25.3°C, respectively). Pigs fed COT had increased ( < 0.05) SFA, PUFA, and iodine value (IV) compared with CON-fed pigs. Glycerol supplementation had no influence on SFA, MUFA, and PUFA concentrations or IV of belly, jowl, and back fat compared with CON. In conclusion, COT diets improved growth performance due to greater energy density, but carcass composition was not affected by treatments. In this experiment, feeding neither COT nor GLY improved fat firmness of pigs fed diets containing 40% DDGS.

Modulation of intestinal cell differentiation in growing pigs is dependent on the fiber source in the diet.

Feeding high-fiber diets decreases cost, but also caloric and nutritional efficiency by modifying intestinal morphology and function. We analyzed the changes in intestinal cell composition, nutrient transporters and receptors, and cell differentiation induced by fibers from different sources. Forty-six finishing pigs (BW 84 ± 7 kg) were fed 1 of 4 diets: corn-soybean (Control; = 12), 23% wheat straw (WS; = 11), 55% corn distillers dried grains with solubles (DDGS; = 11), and 30% soybean hulls (SBH; = 12). Pigs were fed 2 meals daily to an amount equivalent to 2.5% of initial BW for 14 d in metabolism cages. Ilea were collected for histological and gene expression analysis after euthanasia. Data were analyzed using the Kruskal-Wallis test followed by Dunn's multiple comparisons and differences considered significant when < 0.05. The enterocyte marker was increased ( < 0.03) by feeding SBH compared with Control and WS diets. Goblet cells presence was greater ( < 0.01) in pigs fed WS and DDGS compared with Control, and in pigs fed WS compared with SBH ( = 0.02). expression was greater ( < 0.05) in pigs fed DDGS and SBH compared with Control diet. No changes were observed for endocrine and Paneth cells markers, villus and crypt length, or proliferation index. Compared with the Control, gene expression of receptors for oligopeptides, calcium, glucose, fructose, , and and was increased ( < 0.05) by feeding WS and DDGS diets. Feeding SBH diet repressed ( < 0.005) the compared with WS and DDGS diets, while DDGS repressed ( = 0.02) its expression compared with Control. Pigs fed DDGS had reduced ( < 0.001) , and those fed SBH showed increased ( < 0.05) expression compared with WS and DDGS pigs. Feeding WS and DDGS diets induced ( < 0.01) the expression of stem cell marker r-spondin receptor (, while was reduced ( < 0.02) by feeding DDGS compared with Control. The expression of was induced ( < 0.05) by all fibers compared with Control. Transcription factors and were suppressed ( < 0.001) by WS and DDGS compared with Control. In conclusion, feeding diets containing WS and DDGS modulated intestinal differentiation by promoting goblet cells and altered expression of nutrient receptors and transporters in growing pigs, while feeding SBH had less effect.

Differences in in vitro hydrolysis and fermentation among and within high-fiber ingredients using a modified three-step procedure in growing pigs.

In vitro DM disappearance (IVDMD) and gas production can be used to rapidly estimate apparent total tract digestibility of DM and GE in feed ingredients used in swine diets. However, the accuracy of the system in estimating ME among sources feed ingredients with high content of dietary fiber is not clear. Objectives of this study were 1) to measure IVDMD of feed ingredients with high insoluble fiber content and determine and compare in vitro gas production kinetics from fiber fermentation among wheat straw (WS; 16 sources; 69.0-83.4% NDF), soybean hulls (SBH; 16 sources; 60.9-67.7% NDF), and corn distiller's dried grains with solubles (DDGS; 16 sources; 28.8-44.0% NDF); and 2) to estimate ME contributions resulting from gas production of DDGS. Each 2-g sample was hydrolyzed for 2 h with pepsin and for a subsequent 4 h with pancreatin. Hydrolyzed residues were filtered, washed, dried, weighed, pooled within the same sample, and used for subsequent fermentation using swine fecal inocula. Volume of gas produced was recorded at 11 time points during 72 h of incubation. Parameters of gas production kinetics were calculated using a nonlinear monophasic model, and differences among ingredients were compared using a mixed model. The IVDMD from simulated gastric and small intestinal hydrolysis (IVDMDh) in DDGS (55.7%) was greater (P < 0.05) than that in SBH (19.7%), which was greater (P < 0.05) than that in WS (14.5%). In vitro DM digestibility from simulated large intestine fermentation (IVDMDf) of SBH (68.5%) was greater (P < 0.05) than that of DDGS (52.7%), which was greater than that of WS (41.8%). In vitro DM digestibility from simulated total tract digestion (IVDMDt) was greatest (P < 0.01) in DDGS (79.2%) followed by SBH (74.8%), and both were greater than that in WS (50.2%). The asymptotic gas production (mL/g substrate) was greater (P < 0.05) for SBH (293) than for DDGS (208) and WS (53). There were differences (P < 0.01) in IVDMDh among sources of WS, SBH, and DDGS, whereas IVDMDf and IVDMDt were different (P < 0.01) among sources of SBH but not among sources of DDGS or WS. There were no differences in asymptotic gas production among sources of WS, SBH, or DDGS. In conclusion, the modified 3-step procedure allowed for characterizing the variability of DM digestibility and asymptotic gas production resulting from residue fermentation among WS, SBH, and DDGS and among sources of each ingredient.

Use of in vitro dry matter digestibility and gas production to predict apparent total tract digestibility of total dietary fiber for growing pigs.

In vitro DM disappearance (IVDMD) and gas production methods have been developed and used to measure in vivo nutrient digestibility of feed ingredients, but further validation is needed for ingredients containing high concentrations of insoluble fiber such as corn distiller's dried grains with solubles (DDGS). A 3-step in vitro procedure and resulting gas production were used to predict in vivo apparent total tract digestibility (ATTD) of total dietary fiber (TDF) among 3 sources each of wheat straw (WS), soybean hulls (SBH), and DDGS. A total of 34 barrows and 2 gilts (84 ± 7 kg BW) were used in a changeover design to determine the ATTD of 9 dietary treatments. The WS, SBH, or DDGS sources were the only ingredients containing fiber in each diet, and all diets were formulated to contain the same TDF concentration (22.3%). The in vivo experiment was conducted in 2 consecutive 13-d periods, each including a 10-d adaptation and a 3-d collection period to provide 8 replications/dietary treatment, and 0.5% TiO was added to each diet as an indigestible marker. Pigs had ad libitum access to water and were fed an amount of feed equivalent to 2.5% of initial BW in each period. The in vitro experiment was used to determine IVDMD and gas production of the 9 ingredients (5 to 8 replicates/ingredient) fed during the in vivo experiment. Gas production kinetics were fitted using a nonlinear model and analyzed using a mixed model, and predictions were evaluated using correlations and regression models. There were differences ( < 0.01) in ATTD of TDF among WS (26.7%), SBH (78.9%), and DDGS (43.0%) and among sources of DDGS (36.0 to 49.8%). Differences ( < 0.05) in IVDMD from simulated gastric and small intestinal hydrolysis were observed among WS (13.3%), SBH (18.9%), and DDGS (53.7%) and among sources of WS (12.8 to 13.8%), SBH (17.0 to 20.5%), and DDGS (52.0 to 56.9%). Differences ( < 0.05) in IVDMD from simulated large intestine fermentation (IVDMDf) were also observed among WS (23.3%), SBH (84.6%), and DDGS (69.6%) and among sources of WS (18.7 vs. 26.8%). In vitro DM disappearance from simulated total tract digestion of SBH (88.9%) and DDGS (86.1%) were greater ( < 0.01) than that of WS (33.5%). Differences ( < 0.01) in asymptotic gas production (A; mL/g DM substrate) were observed among WS (121), SBH (412), and DDGS (317), and ATTD of TDF was highly correlated with IVDMDf and A. In conclusion, low variability in ATTD of TDF and IVDMD among sources of WS and SBH evaluated in the current study may not justify the use of in vitro measurements, but in vitro fermentation accurately predicts ATTD of TDF among sources of corn DDGS.

Pork fat quality of pigs fed distillers dried grains with solubles with variable oil content and evaluation of iodine value prediction equations.

Back, belly, and jowl fat samples of pigs fed control corn-soybean meal-based diets and diets containing 4 sources of distillers dried grains with solubles (DDGS) were used to determine the impact of feeding DDGS with variable oil content on pork fat quality and to evaluate the precision and accuracy of published iodine value (IV) prediction equations. Dietary treatments consisted of 4 corn-soybean meal diets containing 40% DDGS from different sources with 10.7, 5.6, 14.2, or 16.0% ether extract (EE; as-fed) content. Diets did not contain any other supplemental lipid sources. Regardless of fat depot, SFA content (g/100 g fat) of pigs fed 5.6% EE DDGS (35.4) was greater ( < 0.05) than that of pigs fed 14.2 or 16.0% EE DDGS sources (34.4 and 30.2, respectively) and tended to be greater ( < 0.10) than that of pigs fed 10.7% EE DDGS (34.6). Pigs fed 10.7 and 14.2% EE DDGS had greater ( < 0.01) SFA concentration than pigs fed 16.0% EE DDGS. Regardless of fat depot, MUFA content (g/100 g fat) of pigs fed 10.7, 5.6, and 14.2% DDGS sources were similar (43.7, 43.1, and 43.0, respectively) but were greater ( < 0.01) than that of pigs fed 16.0% EE DDGS (40.0). A dietary treatment × fat depot interaction was observed for PUFA ( < 0.05) and IV ( = 0.079). Pigs fed 10.7, 5.6, and 14.2% DDGS sources had reduced ( < 0.01) PUFA concentration and IV compared with pigs fed 16.0% EE DDGS, but the magnitude of responses in PUFA and IV to the variable oil content of DDGS was greater in backfat than in belly and jowl fat. Carcass fat IV data were used to evaluate prediction error (PE) and bias of published carcass fat IV prediction equations. Equations using dietary C18:2 content or IV product as a single predictor resulted in highly variable PE (g/100 g) ranging from 3.43 to 8.36 and bias (g/100 g) ranging from -5.05 to 5.66. Using equations that included additional diet composition information and pig growth performance factors decreased PE (3.27 to 4.73) and bias (-3.37 to 1.73) of prediction for backfat compared with equations only based on the characteristics of dietary lipid, but this improvement was limited in the prediction for belly and jowl fat. Predictions based on percentage of DDGS in diets had the greatest PE (6.66 to 9.19) and bias (5.53 to 8.00).

Effects of feeding diets containing distillers' dried grains with solubles and wheat middlings with equal predicted dietary net energy on growth performance and carcass composition of growing-finishing pigs.

This experiment evaluated the effects of feeding distillers' dried grains with solubles (DDGS) and wheat middlings (WM) in diets with similar estimated NE content on growth performance and carcass characteristics of growing-finishing pigs. Pigs ( = 384; 29.1 ± 3.6 kg initial BW) were blocked by initial BW, and within blocks, pens were randomly allotted to 1 of 4 dietary treatments (8 pigs/pen and 12 replicates/treatment) in a 4-phase feeding program (29 to 50, 50 to 75, 75 to 100, and 100 to 120 kg BW). Dietary treatments were arranged in a 2 × 2 factorial design and formulated to consist of: 1) corn and soybean meal (CON), 2) CON with 30% DDGS, 3) CON with 15% WM, and 4) CON with 30% DDGS and 15% WM. Diets met or exceeded nutrient requirements published by the and were formulated to contain the same concentrations of standardized ileal digestible (SID) Lys and meet or exceed minimum SID AA to Lys ratios within phases. No significant interactions for DDGS × WM × phase or DDGS × WM were observed for all growth performance criteria. Pigs fed diets containing 30% DDGS had reduced ( < 0.05) ADFI (1.76 vs. 1.86 kg/d) and ADG (0.85 vs. 0.91 kg/d) compared with pigs fed diets with no DDGS in phase 1 but not in other phases. The G:F of pigs fed diets containing DDGS was not different during phase 1 to 3 but was greater ( < 0.01) in phase 4 (0.313 vs. 0.291) compared with that of pigs fed diets with no DDGS. Pigs fed diets containing 15% WM had similar ADFI and G:F but reduced ( < 0.05) ADG (0.86 vs. 0.90 kg/d) compared with pigs fed diets without WM in phase 1, but no difference in ADG was observed in phases 2 to 4. No DDGS × WM interaction was observed for carcass traits. Pigs fed diets containing 30% DDGS had reduced ( < 0.01) HCW (86.5 vs. 89.9 kg), carcass yield (72.3 vs. 73.6%), LM area (45.0 vs. 47.9 cm), and percentage of carcass fat-free lean (52.1 vs. 53.4%), but backfat depth was not affected compared with pigs fed diets with no DDGS. Pigs fed diets containing 15% WM had lower carcass yield (72.7 vs. 73.1%; < 0.05) and HCW (87.7 vs. 88.7 kg; < 0.10) than pigs fed diets with no addition of WM, but other carcass traits were not affected. In conclusion, overall ADG and G:F were not affected by feeding 30% DDGS or 15% WM when diets were formulated on the NE basis, but more accurate and dynamic estimation of NE content for DDGS sources is needed to optimize caloric efficiency at different physiological ages of pigs.

Impact of synthetic antioxidants on lipid peroxidation of distiller's dried grains with solubles and distiller's corn oil stored under high temperature and humidity conditions.

This experiment evaluated the effect of antioxidants, oil content in distiller's dried grains with solubles (DDGS), quality of distiller's corn oil, and storage time on lipid peroxidation. A source of low-oil DDGS (LO-DDGS; 5.0% ether extract [EE], as-fed basis), high-oil DDGS (HO-DDGS; 13.0% EE, as-fed basis), and 2 sources of distiller's corn oil (DCO; 1.20, 0.08, and 0.48% moisture, insoluble impurities, and unsaponifiables [MIU], respectively [DCO-1], and 1.20, 0.01, and 0.10% MIU, respectively [DCO-2]) were obtained. Each of the 4 ingredients was divided into 18 representative subsamples (approximately 908 g for DDGS or 2 kg of DCO). Six subsamples of each ingredient were mixed with either no supplemental antioxidants (CON), Rendox-CQ (REN; 1,000 mg/kg EE; Kemin, Industries, Des Moines, IA), or Santoquin-Q4T (SAN; 1,500 mg/kg EE; Novus International, St. Louis, MO). Each mixture ( = 72) was split into thirds, and 1 portion was immediately frozen at -20°C (d 0). Two portions were stored under hot (38.6 ± 0.1°C) and humid conditions (94.0 ± 0.3% relative humidity) for 14 or 28 d. The MIXED procedure of SAS was used to evaluate the effects of ingredient, antioxidant, storage time, and interactions, with d-0 values used as a covariate. From d 14 to 28, peroxide value (PV), -anisidine value (AnV), and thiobarbituric acid reactive substances (TBARS) of DCO and DDGS increased by 3- to 4-fold ( < 0.05). Over the entire storage period, PV of DCO-1 and HO-DDGS (12.3 ± 0.3 and 12.6 ± 0.3 mEq O/kg oil, respectively) exceeded ( < 0.05) that of DCO-2 and LO-DDGS (9.6 ± 0.3 and 9.3 ± 0.3 mEq O/kg oil, respectively). Adding REN or SAN ( < 0.05) reduced TBARS and AnV relative to CON (TBARS = 11.0 ± 0.2 mg malondialdehyde Eq/kg oil and AnV = 6.5 ± 0.2) over the entire period (mean of d 14 and 28), but TBARS and AnV did not differ ( > 0.05) between antioxidants (TBARS = 6.1 ± 0.2 and 5.9 ± 0.2 mg malondialdehyde Eq/kg oil, respectively, and AnV = 1.9 ± 0.2 and 1.8 ± 0.2 for REN and SAN, respectively). The PV on d 14 and 28 and overall was less ( < 0.05) when either antioxidant was added relative to CON (16.0 mEq O/kg) and was greater for ingredients treated with SAN ( < 0.05) compared with REN (8.8 ± 0.2 and 8.0 ± 0.2 mEq O/kg oil for SAN and REN, respectively). In summary, antioxidants reduced peroxidation of DDGS and DCO by approximately 50% during 28 d of storage at 38.6°C and 94.0% relative humidity, but neither antioxidant completely stabilized the ingredients.

Effects of adding supplemental tallow to diets containing distillers dried grains with solubles on fatty acid digestibility in growing pigs.

An experiment was conducted to measure the apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of fatty acids in diets containing 0 or 30% corn distillers dried grains with solubles (DDGS) and 0, 5, or 10% tallow. Barrows (n = 24; initial BW = 25 kg) were surgically fitted with a T-cannula at the distal ileum. Pigs (n = 4/diet) were randomly assigned to diets: corn-soybean meal control (CON), CON plus 5% tallow (5T0D), CON plus 10% tallow (10T0D), CON plus 30% DDGS (0T30D), CON plus 5% tallow and 30% DDGS (5T30D), and CON plus 10% tallow and 30% DDGS (10T30D). Eight replicates per treatment were achieved by randomizing diets among pigs for a second collection period. Each pig was fed their respective diet for a 5-d adaptation period followed by 3-d fecal collection and 2-d ileal digesta collection periods. The AID and ATTD of fatty acids was calculated using the index method and acid-insoluble ash as an indigestible marker. When tallow was added to diets with 0% DDGS, there was no effect on AID of palmitic acid (C16:0) or SFA, while AID of stearic acid (C18:0) was increased (66.87% for CON, 72.06% for 5T0D, and 76.81% for 10T0D; P < 0.01). However, when diets contained 30% DDGS, the AID of all SFA was reduced as levels of tallow increased C16:0 (77.62% for 0T30D, 69.66% for 5T30D, and 68.43% for 10T30D), C18:0 (85.87% for 0T30D, 64.08% for 5T30D, and 61.25% for 10T30D), and SFA (79.88% for 0T30D, 68.23% for 5T30D, and 66.29% for 10T30D). The AID of MUFA was not affected when tallow was added to diets with 30% DDGS but actually increased in 5T0D and 10T0D. The amount of apparent ileal digested fatty acids increased with the addition of DDGS and tallow regardless of their digestibility. Amounts of ileal digested MUFA and PUFA increased when both DDGS (P < 0.01) and tallow (P < 0.01) were included in the diet compared to when either ingredient was excluded. For ileal digestible SFA, an interaction (P < 0.01) between DDGS and tallow was observed. Interactions between DDGS and tallow were observed for ATTD of all fatty acids (P < 0.01), except for linoleic acid and PUFA, which were not affected by dietary treatment. The amount of ileal digestible PUFA increased with tallow addition independent from the inclusion level of DDGS and it was a function the dietary concentration of the fatty acid. Adding tallow to corn-soybean based diets increased the amount of digested SFA, but in diets with DDGS, the increment in amount of ileal digestible SFA was less prominent due to lesser digestibility of SFA.

Effects of adding supplemental tallow to diets containing 30% distillers dried grains with solubles on growth performance, carcass characteristics, and pork fat quality in growing-finishing pigs.

Crossbred pigs (n = 315) were blocked by initial BW (6.8 ± 1.1 kg) and randomly assigned to 1 of 4 dietary treatments to evaluate the effects of dietary inclusion of tallow and corn distillers dried grains with solubles (DDGS) on pig growth, carcass traits, and pork fat quality. Diets consisted of a corn-soybean meal control diet (CON) and another 3 corn-soybean meal diets containing 5% tallow (T), 30% DDGS (D), or 5% tallow plus 30% DDGS (TD) in a 2 × 2 factorial arrangement of treatments. Diets were formulated to contain similar levels of available P and standardized ileal digestible Lys:ME among treatments. Pigs were housed in 40 pens, with 7 to 8 pigs per pen, to provide 10 replicates per treatment. Overall ADG did not differ among treatments. Compared with CON (2.76 kg/d) and T (2.59 kg/d), feeding 30% DDGS reduced the ADFI (interaction, P > 0.05) of pigs when fed with 5% tallow (2.45 kg/d for TD) but not when fed alone (2.76 kg/d for D). There was no effect of DDGS on overall G:F, but pigs fed diets with tallow had greater (P < 0.01) G:F (0.4) than pigs fed no tallow (0.37). Feeding tallow increased (P < 0.01) HCW, carcass yield, and backfat depth of pigs independent of DDGS. Feeding DDGS reduced (P < 0.01) belly firmness, as measured by belly flop angle, independent of tallow (D = 71.8° and TD = 57.7° vs. CON = 134.0° and T = 113.4°) and tallow also tended to reduce belly firmness (P < 0.10). Feeding DDGS and tallow reduced the concentration of SFA in belly fat, while the concentration of MUFA were increased (P < 0.01) by feeding tallow but not DDGS. Conversely, feeding DDGS increased (P < 0.01) the concentration of PUFA in belly fat but there was no effect of tallow. An interaction (P = 0.03) between DDGS and tallow for iodine value (IV) of belly fat was observed, in which addition of tallow or DDGS increased the IV of belly fat (64.22 for T and 71.22 for D vs. 59.01 for CON) but addition of both reduced IV (67.88 for TD). The IV of belly fat and backfat were correlated (P < 0.01) with the IV product of the diet fed in phase 3 (r = 0.49 and r = 0.81, respectively). In conclusion, adding 5% tallow to 30% DDGS diets improved G:F and carcass yield while reducing the IV of belly fat. However, pork fat firmness as measured by belly flop angle was not improved.

Prediction of the concentration of standardized ileal digestible amino acids in distillers dried grains with solubles.

Values for the concentration of standardized ileal digestible (SID) CP, Lys, Met, Thr, and Trp from 34 sources of distillers dried grains with solubles (DDGS) were obtained from a series of 5 experiments with the objective of predicting the concentration of SID AA from physical and chemical assays. The concentration of NDF, ADF, hemicellulose, acid detergent insoluble CP (ADICP), and KOH soluble protein (SolCP) were measured and calculated in all DDGS sources. Likewise, particle size was measured and color of each source of DDGS was determined with a Minolta colorimeter and HunterLab spectrometer and was expressed as lightness (L*), redness (a*), and yellowness (b*). The HunterLab spectrometer also provided optical density that was recorded between 400 and 700 nm. Front face fluorescence was measured at 360 nm excitation and recorded from 380 to 600 nm. Multiple linear regression and principal components analyses were performed to predict the concentration of SID AA among DDGS sources, and predicted means as well as predicted residual sums of squares (PRESS) were calculated to estimate accuracy and precision of the model. Some correlations (P < 0.05) were observed between ADF, hemicellulose, ADICP, and SolCP with SID CP and AA but were generally low (r < 0.51). There was a greater association (R(2) = 0.40; P < 0.05) between L* and SID Lys among DDGS sources when L* was less than 50 than when samples had L* values greater than 50. In addition, a* was negatively correlated (P < 0.05) with SID CP (r = -0.41), Lys (r = -0.59), and Met (r = -0.50) whereas b* tended to be positively correlated (P < 0.10) with SID Lys (r = 0.31) and Trp (r = 0.30) and was correlated (P = 0.05) with SID Met (r = 0.43) and Thr (r = 0.36). There were no correlations between NDF or particle size with SID CP and AA. Optical density, along with CP, was highly predictive of SID Lys (R(2) = 0.97; PRESS = 0.05), Thr (R(2) = 0.94; PRESS = 0.06), and Trp (R(2) = 0.93; PRESS = 0.004) but not SID Met (R(2) = 0.39; PRESS = 0.12). Front face fluorescence was also highly predictive of SID Lys (R(2) = 0.99; PRESS = 0.07), Met (R(2) = 0.95; PRESS = 0.05), Thr (R(2) = 0.99; PRESS = 0.008), and Trp (R(2) = 0.99; PRESS = 0.006). In conclusion, correlations between ADICP, SolCP, NDF, particle size, and color measurements with SID AA concentrations were poor, but optical density and front face fluorescence methods appear to provide good predictions of SID AA concentrations in DDGS. However, these prediction equations need to be validated using samples of DDGS from a separate data set.

Comparative digestibility of energy and nutrients in fibrous feed ingredients fed to Meishan and Yorkshire pigs.

The objective of this experiment was to test the hypothesis that differences in the digestibility of total dietary fiber among breeds of pigs is influenced by the type of fiber fed and also by the age of the pig. Five Meishan pigs (BW: 77.2 ± 15.2 kg; 5 mo old), 5 light Yorkshire pigs (BW: 80.1 ± 11.2 kg; 4 mo old), and 5 heavy Yorkshire pigs (BW: 102.1 ± 3.5 kg, 5 mo old) were surgically prepared with a T-cannula in the distal ileum. A corn-soybean meal diet (control) was formulated with 5 g•kg(-1) of titanium dioxide as an indigestible marker. Three additional diets were formulated by replacing 30% of the control diet with 30% of distillers dried grains with solubles (DDGS), soybean hulls, or sugar beet pulp, and 1 diet was formulated by replacing 15% of the control diet with 15% pectin. Each group of pigs was allotted to a 5 × 5 Latin square design, and pigs were fed the 5 experimental diets during five 14-d periods. Fecal samples were collected on d 12, and ileal digesta were collected on d 13 and 14 of each period. The apparent ileal digestibility (AID) and apparent total tract digestibility (ATTD) of GE and nutrients in each ingredient were calculated using the substitution method. Hindgut disappearance was calculated as the difference between ATTD and AID. When fed the control diet, Meishan pigs tended (P < 0.10) to have a greater AID of GE and CP (78.6 and 80.3%, respectively) than light (77.0 and 78.9%, respectively) and heavy (75.7 and 76.9%, respectively) Yorkshire pigs, and they had a greater (P < 0.05) ATTD of DM, GE, and carbohydrates (89.2, 89.5, and 95.5%, respectively) than light (86.6, 86.4, and 92.4%, respectively) and heavy (87.0, 86.6, and 93.0%, respectively) Yorkshire pigs. The ATTD of DM, GE, CP, carbohydrates, and total dietary fiber in DDGS (75.4, 76.3, 81.3, 78.0, and 75.3%, respectively) was greater (P < 0.01) in Meishan pigs than in light (55.7, 58.5, 66.7, 49.2, and 39.0%, respectively) and heavy (59.8, 62.9, 70.0, 51.1, and 55.7%, respectively) Yorkshire pigs. There were no differences among the 3 groups of pigs in the ATTD of GE or nutrients in soybean hulls, sugar beet pulp, or pectin. The hindgut disappearance of DM and carbohydrates in DDGS by Meishan pigs (26.8 and 52.9%, respectively) was greater (P < 0.05) than in the light (10.0 and 22.8%, respectively) and Heavy Yorkshire pigs (12.2 and 20.0%, respectively), but for the other ingredients, no differences in hindgut disappearance among Meishan, light Yorkshire, and heavy Yorkshire pigs were observed. In conclusion, Meishan pigs have a greater ATTD of DM, GE, and some nutrients in corn-soybean meal diets and in DDGS than Yorkshire pigs.

Digestibility of dietary fiber in distillers coproducts fed to growing pigs.

The objective of this work was to measure the apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of dietary fiber in different sources of distillers dried grains with solubles (DDGS) and to calculate hindgut fermentation of dietary fiber in DDGS fed to growing pigs. Diets, ileal digesta, and fecal samples from pigs fed corn or diets containing 1 of 28 sources of distillers coproducts were analyzed for fiber. Of the 28 sources of coproducts, 24 sources were corn DDGS (C-DDGS), 1 source was sorghum DDGS (S-DDGS), 1 source was DDGS from a blend of sorghum and corn (SC-DDGS), 1 source was C-DDGS from beverage production (DDGS(beverage)), and a source of corn distillers dried grain (DDG) was also included in the experiment. Total dietary fiber (TDF) and DM were analyzed in all DDGS sources, ileal digesta, and fecal samples. Hindgut fermentation was calculated by subtracting values for AID from values for ATTD. In 10 sources of DDGS and in ileal and fecal samples from pigs fed those sources, crude fiber, ADF, NDF, insoluble dietary fiber (IDF), and soluble dietary fiber (SDF) were also determined. Concentrations of CP, ether extract, and ash were also analyzed in these samples, and concentrations of organic residue (OR) were calculated by subtracting the concentration of CP, ether extract, and water from OM. The AID and the ATTD of TDF differed (P < 0.01) among sources of C-DDGS. The average AID of TDF in 10 sources of C-DDGS (21.5%) was not different (P > 0.05) from the AID of TDF in corn (16.5%), but the ATTD and the hindgut fermentation of TDF in the 10 sources of C-DDGS (44.5 and 23.0%, respectively) were greater (P < 0.05) than in corn (23.1 and 6.6%, respectively). The AID of crude fiber, NDF, IDF, SDF, and TDF were not different between C-DDGS and S-DDGS, but the AID of ADF was greater (P < 0.01) in S-DDGS (57.4%) than in C-DDGS (36.8%). The ATTD of OR in S-DDGS (72.5%) and SC-DDGS (68.4%) were less (P < 0.05) than in C-DDGS (77.1%), but the ATTD of ADF, NDF, IDF, SDF, and TDF were not different among the 3 sources of DDGS. The AID, ATTD, and hindgut fermentation of TDF were not different between DDGS from an ethanol plant and DDGS from a beverage plant. The average AID, ATTD, and hindgut fermentation of TDF in the 24 sources of C-DDGS were 23.0, 47.3, and 24.4%, respectively. It is concluded that the AID and ATTD of fiber differ among sources of DDGS and those differences may contribute to differences in the digestibility of energy in DDGS.

Effects of distillers dried grains with solubles on amino acid, energy, and fiber digestibility and on hindgut fermentation of dietary fiber in a corn-soybean meal diet fed to growing pigs.

The objective of this experiment was to measure the effect of distillers dried grains with solubles (DDGS) on the digestibility of AA, energy, and fiber, on the fermentation of fiber, and on the first appearance of digesta at the end of the ileum, in the cecum, and in the feces of growing pigs fed a corn-soybean meal-based diet. Sixteen pigs (initial BW = 38.0 +/- 1.6 kg) were prepared with a T-cannula in the distal ileum and a T-cannula in the cecum and allotted to 2 treatments. In period 1, all pigs were fed a corn-soybean meal diet. In periods 2, 3, and 4, pigs were fed the control diet or a diet containing corn, soybean meal, and 30% DDGS. First appearance of digesta at the end of the ileum, in the cecum, and over the entire intestinal tract was measured at the end of period 4. The apparent ileal digestibility (AID) and the apparent total tract digestibility (ATTD) of nutrients were measured, and the concentration of VFA was analyzed in ileal, cecal, and fecal samples. The AID of Lys (74.1%) in the DDGS diet was less (P < 0.05) than in the control diet (78.6%), but the AID of most other AA and GE, NDF, and total dietary fiber (TDF) were not different between the 2 diets. The ATTD of GE (81.0%), NDF (57.2%), TDF (55.5%), and DM (81.7%) were less (P < 0.05) in the DDGS diet than in the control diet (86.0, 69.3, 66.0, and 87.2%, respectively). The concentration of VFA in ileal, cecal, and fecal samples was not different between pigs fed the 2 diets. The pH of ileal and cecal digesta from pigs fed the DDGS diet (6.3 and 5.5) was greater (P < 0.01) than from pigs fed the control diet (5.8 and 5.3). The ATTD of DM, GE, ADF, NDF, and TDF did not change with collection period, but the AID of ADF, NDF, and TDF increased (P < 0.05) from period 2 to period 4. The concentration of all VFA, except isobutyrate, was greater (P < 0.05) in cecal samples from period 4 compared with period 2, and the concentration of all VFA except propionate and isovalerate were greater (P < 0.05) in fecal samples collected in period 4 compared with those collected in period 2. The first appearance of digesta at the end of the ileum, in the cecum, and in the feces was not affected by DDGS. In conclusion, pigs fed the diet containing DDGS had less digestibility of Lys, GE, ADF, NDF, and TDF than pigs fed the control diet. The digestibility of DM and GE was not influenced by collection period, but the concentration of VFA in cecal digesta and feces increased with the length of time pigs received the diets.