RESUMO
BACKGROUND: Hepatitis C virus (HCV) infection is an important cause of liver cancer in Thailand. The highest prevalence of anti-HCV positive among Thai blood donors is found in the northeastern region. The present analysis of the genotype distribution among anti-HCV positive northeastern-Thai blood donors was conducted to provide a base for the epidemiological pattern of HCV infection in this region. MATERIALS AND METHODS: A total of 112 HCV seropositive healthy blood donors were randomly selected and tested for the presence of HCV-RNA by RT-PCR. HCV-RNA positive samples were genotyped by direct sequencing at core region genomes and confirmed by phylogenetic analysis. RESULTS: HCV viremia was found in 94.6% (106/112) of HCV seropositive blood donors. There were 3 major genotypes distributed among this population. HCV genotype 3a was the most prevalent (71.7%) followed by genotypes 1a (7.5%), 1b (7.5%), 6i (3.8%), 6f (2.8%) and 6n (1.9%). CONCLUSIONS: HCV genotype 3a in asymptomatic infections in northeastern Thailand is significantly higher than other previous reports. Subgenotype 6 prevalence is less than in neighboring countries and distribution patterns differ. The findings are relevant as predictors for using interferon therapy in this population.
Assuntos
Doadores de Sangue , Hepacivirus/genética , Hepatite C/epidemiologia , Hepatite C/virologia , RNA Viral/genética , Adulto , Feminino , Seguimentos , Genótipo , Hepacivirus/isolamento & purificação , Hepatite C/genética , Humanos , Masculino , Prevalência , Prognóstico , RNA Viral/sangue , Estudos Soroepidemiológicos , Tailândia/epidemiologiaRESUMO
Data of the prevalence of red cell alloantibodies in blood donors will assist in improving safe blood supply for patients needing transfusion. The purpose of this study was to determine prevalence of red cell alloantibodies in Lao blood donors. Antibody screening and identification by multiple phase indirect antiglobulin standard tube assay were performed in serum samples from 1,181 blood donor individuals following routine work at the Lao Red Cross National Blood Transfusion Center. The overall prevalence of alloantibodies was 3.90%. All of the antibodies found were naturally occurring antibodies. Anti-P1 was the most common with a frequency of 1.95% followed by anti-Le(a), anti-Le(b), anti-Le(a+b), anti-M, anti-P1 plus anti-Le(a), anti-P1 plus anti-Le(a+b) with the prevalence of 0.59%, 0.42%, 0.42%, 0.17%, and 0.08%, respectively. This is the first report about prevalence of red cell alloantibodies among Lao blood donors. The information obtained from this study will be useful for safe blood transfusion and preparation of in house antisera.
Assuntos
Doadores de Sangue , Eritrócitos/imunologia , Isoanticorpos/sangue , Feminino , Humanos , Laos , Masculino , PrevalênciaRESUMO
Semi-nested polymerase chain reaction technique was used to detect Torquetenovirus (TTV) DNA in 234 healthy blood donors in northeast Thailand. The incidence of TTV was 28% in 101 healthy blood donors negative for HBsAg and anti-HCV antibody, 25% in 71 HBsAg carriers and 29% among 62 with anti-HCV antibody. No association of TTV infection was found with gender, age, and HBV or HCV infection.
Assuntos
Doadores de Sangue/estatística & dados numéricos , Infecções por Vírus de DNA/epidemiologia , DNA Viral/sangue , Torque teno virus/isolamento & purificação , Adolescente , Adulto , Idoso , Infecções por Vírus de DNA/diagnóstico , Infecções por Vírus de DNA/genética , DNA Viral/genética , Feminino , Antígenos de Superfície da Hepatite B/sangue , Antígenos de Superfície da Hepatite B/genética , Anticorpos Anti-Hepatite C/sangue , Anticorpos Anti-Hepatite C/genética , Humanos , Incidência , Masculino , Pessoa de Meia-Idade , Reação em Cadeia da Polimerase , Tailândia/epidemiologia , Torque teno virus/genéticaRESUMO
Hepatitis G viral (HGV) infection among northeastern Thai blood donors was determined by the nested RT-PCR technique. HGV RNA was amplified by the degenerated helicase primers for a product of the expected size of 83 base pairs were used in this study. Serum samples from 322 of three different categories of northeastern Thai blood donors were included in this study. There were 104 HBsAg and Anti-HCV seronegative blood donors (control group), 100 samples of HBs Ag seropositive blood donors (HBV infected group) and 118 serum samples from anti-HCV seropositive blood donors (HCV infected group). The results demonstrated that HGV RNA was not detected in the control group but was found in 10 individuals (10%) in the HBV infected group and 13 (11%) in the anti-HCV positive blood donors. The prevalences of HGV in both seropositive groups were significantly different from the control group (p = 0.001). HGV co-infection is highly prevalent among northeastern Thai blood donors who are infected with HBV or HCV. The results also reveal that blood donors seronegative for HCV and HBV are a low risk group for HGV infection.
