The effects of different dietary protein sources on live weight, sperm quality and the histology of the testes and accessory glands in male rats.

This study was aimed at determining the effects of corn and wheat gluten, used as dietary protein sources, on live weight gain, sperm quality and the histology of the testes and accessory glands in male rats. For this purpose, 20-day-old 24 male Wistar albino rats were randomly assigned to three groups. Group 1 (Control), Group 2 and Group 3 were fed on a basal ration supplemented with high levels of soybean meal, corn gluten and wheat gluten, respectively, as a protein source. At the end of the study, when compared to Group 1, live weight values were determined to have increased in Group 3 and to have decreased in Group 2 (p < .05). Furthermore, sperm density, sperm motility, the dead/ live sperm ratio and testes weight were determined to have significantly decreased in Group 2, in comparison to Groups 1 and 3 (p < .05). The percentages of abnormal spermatozoon, and head, acrosome, mid-piece and tail abnormalities were high in Group 2 (p < .05). Histological examination demonstrated that, in Group 2, the diameter of the Tubulus Seminiferous Contortus (TSC) and the size of the Tubular Epithelial Cells (TE) were small, and the tubular and anatomical structure of the testes were shrunken and altered. Group 2 also presented with connective tissue increase and alveolar lumen enlargement in the prostate gland, and with connective tissue thickening, muscle tissue increase and secretory capacity decrease in the seminal vesicle (p < .05). Moreover, in Group 2, the Gl. Bulbourethral (Cowper's gland) presented with a decreased size and dilatations in the mucous structures. In a result, based on the findings obtained in this study, it is suggested that high levels of dietary corn gluten adversely affect live weight, sperm quality, and the testes and accessory glands.

Postpartum uterus involution observed by real-time ultrasound scanning and vaginal cytology in Van cats.

OBJECTIVES: The objective was to investigate postpartum uterus involution by real-time ultrasonography and vaginal cytology in Van cats. METHODS: This study included 15 healthy Van cats belonging to the Van Cat Research Centre (Yuzuncu Yil University, Van, Turkey). Starting 24 h postpartum, ultrasonographic measurements were performed on the placental and interplacental uterine horn regions every day. Decreases in the diameters and uterine content were considered as criteria for uterine involution. Vaginal discharge samples were collected every day for 4 weeks postpartum. The smears were stained with Papanicolaou stain. RESULTS: The average diameters of placental and interplacental regions (IPRs) in the uterine horns were 3.12 ± 0.29 cm and 2.36 ± 0.43 cm, respectively, at 24 h postpartum. Placental regions (PRs) shrank faster than IPRs. At 48 h postpartum, it became difficult to distinguish PRs from IPRs in the uterine horns. The uterine horns could be seen in the abdominal cavity up to 5.60 ± 0.99 days postpartum. The mean of the last assessable diameter of the uterine horns from days 4 to 7 in all cats was 0.49 ± 0.07 cm. The vaginal epithelial cells appeared to be under the effect of oestrogen for 4 weeks postpartum. CONCLUSIONS AND RELEVANCE: The morphological involution of the uterus completes, to a large extent, within the first 48 h postpartum in Van cats. A more detailed hormonal analysis would contribute greatly to the understanding of the physiological processes involved in this period. Although postpartum involution appeared complete by 5.60 ± 0.99 days after parturition in Van cats, histological verification of this finding is needed.