Comprehensive data analysis of white blood cells with classification and segmentation by using deep learning approaches.

Deep learning approaches have frequently been used in the classification and segmentation of human peripheral blood cells. The common feature of previous studies was that they used more than one dataset, but used them separately. No study has been found that combines more than two datasets to use together. In classification, five types of white blood cells were identified by using a mixture of four different datasets. In segmentation, four types of white blood cells were determined, and three different neural networks, including CNN (Convolutional Neural Network), UNet and SegNet, were applied. The classification results of the presented study were compared with those of related studies. The balanced accuracy was 98.03%, and the test accuracy of the train-independent dataset was determined to be 97.27%. For segmentation, accuracy rates of 98.9% for train-dependent dataset and 92.82% for train-independent dataset for the proposed CNN were obtained in both nucleus and cytoplasm detection. In the presented study, the proposed method showed that it could detect white blood cells from a train-independent dataset with high accuracy. Additionally, it is promising as a diagnostic tool that can be used in the clinical field, with successful results in classification and segmentation.

Do hypomineralized teeth affect parents' emotional states and attitudes more than other discoloration conditions?

Discoloration poses a significant esthetic issue, and parents' opinions and emotional states may vary regarding different types of discoloration, such as molar incisor hypomineralization, caries or chromogenic bacteria. This study aimed to assess parent's emotional state and attitudes towards discoloration in primary and permanent teeth. A two-part questionnaire was designed, consisting of questions about demographic data and simulated visuals of different primary and permanent teeth discoloration conditions. The Visual Analog Scale (VAS) was used to determine the emotional state, while parents' attitudes towards dental consultation for each simulated visual were determined using statements such as "Absolutely yes"; "Yes"; "I don't have an idea"; "No"; "Absolutely no". The simulated visuals included cases of "caries with cavitation", "yellow-brown hypomineralized area", "white hypomineralized area" and "chromogenic bacteria". The questionnaire was distributed to 300 parents through online communication channels. Categorical variables were analyzed using Pearson chi-square, with statistical significance set as p < 0.05. A total of 230 parents completed the questionnaire. The highest VAS scores and the highest percentage of "Absolutely yes" responses were observed for "caries with cavitation" in both primary (77.8%, 81.7%) and permanent (60.4%, 94.3%) teeth. Additionally, statistically significant differences were found in the VAS scores for chromogenic bacteria (p = 0.04), caries with cavitation (p = 0.005), white hypomineralized area (p = 0.01) and yellow-brown hypomineralized area (p = 0.02). Comparatively, parental preferences showed statistically significant differences for chromogenic bacteria (p = 0.01), caries with cavitation (p = 0.01), yellow-brown hypomineralized area (p = 0.01) and white hypomineralized area (p = 0.004). Parents displayed stronger emotional responses towards "caries with cavitation" compared to hypomineralization and chromogenic bacteria discolorations and expressed a positive attitude towards seeking dental consultation.

Novel Electrospun Polymeric Nanofibers Loaded Different Medicaments as Drug Delivery Systems for Regenerative Endodontics.

BACKGROUND: A combination of antibiotics, including metronidazole (MET), ciprofloxacin (CIP), and minocycline (MINO), has been demonstrated to disinfect bacteria in necrotic teeth before regenerative processes. It has been presented clinically that antibiotic pastes may drive to possible stem cell death, creating difficulties in removing from the canal system, which can limit the regenerative procedure. This study was designed to (1) synthesize nanofibrous webs containing various concentrations of different medicaments (triple, double, and calcium hydroxide, Ca(OH)2), and (2) coat the electrospun fibrous gutta-percha (GP) cones. METHODS: Poly(vinylpyrrolidone) (PVP)-based electrospun fibrous webs were processed with low medicament concentrations. Scanning electron microscopy (SEM), energy dispersive X-ray spectroscopy (EDX), and X-ray photoelectron spectroscopy (XPS) were carried out to investigate fiber morphology and antibiotic incorporation, and characterize GP-coated fibrous webs, respectively. The chemical and physical properties of dentine were determined via fourier transform infrared spectroscopy (FTIR) and Nano-SEM, respectively. The antimicrobial properties of the different fibrous webs were assessed against various bacteria by direct nanofiber/bacteria contact. Cytocompatibility was measured by applying the MTT method. RESULTS: The mean fiber diameter of the experimental groups of medicament-containing fibers ranged in the nm scale and was significantly smaller than PVP fibers. EDX analysis confirmed the presence of medicaments in the nanofibers. XPS analysis presented a complete coating of the fibers with GPs; FTIR and Nano-SEM showed no chemical and physical configuration of intracanal medicaments on the dentine surface. Meanwhile, nanofibrous webs led to a significant reduction in the percentage of viable bacteria compared to the negative control and PVP. CONCLUSION: Our findings suggest that TA-NFs, DA-NFs, and Ca(OH)2)-NFs coated GP cones have significant potential in eliminating intracanal bacteria, having cell-friendly behavior and clinical usage features.

Attitudes and emotional status of parents regarding traumatic dental injuries.

BACKGROUND/AIMS: Traumatic dental injuries have different effects on children and their parents, depending on the type of trauma and whether it is in primary or permanent teeth. Parents do not always seek immediate intervention for their children after each traumatic dental injury unless accompanied by conditions such as pain or bleeding that will increase emotional stress. The aim of this study was to evaluate the emotional status of parents and parents' attitudes toward urgent intervention for different traumatic dental injuries in both primary and permanent teeth. MATERIAL AND METHODS: A questionnaire consisting of two parts was designed with a reliability coefficient of 0.87 according to the results of a pilot study. The questionnaire was sent to parents as a Google Form via online communication methods such as WhatsApp and e-mail. The first part included questions about the demographic data of the parents and children, and the second part consisted of images of traumatic dental injuries of primary and permanent teeth with questions to evaluate the emotional state of the parents and awareness of the necessity for urgent intervention. The Visual Analog Scale (VAS) was used to evaluate the emotional state, and "Absolutely yes;" "Yes;" "I don't have an idea;" "No;" and "Absolutely no" statements were used to evaluate parents' attitudes about urgent intervention. Categorical variables were shown as numbers and percentages and analyzed with Pearson chi-square. Statistical significance was evaluated for p < .05, and correlations between variables were calculated with Pearson correlation. RESULTS: The survey was completed by 845 parents. All traumatic injuries except extrusion (p = .202; p ≥ .05) had statistically different VAS scores for primary and permanent teeth (p < .05). Parents' attitudes for urgent intervention were statistically significant for all traumatic injuries (p < .05) except 4-mm intrusion (p = .062; p ≥ .05), alveolar fracture (p = .282; p ≥ .05), complicated crown fracture (p = .136; p ≥ .05), and non-traumatized healthy teeth (p = .110; p ≥ .05). CONCLUSIONS: Traumatic dental injuries with excessive bleeding or tissue loss affect the emotional status of parents more than simple injuries, and they prefer to refer to dentists immediately. Parents responded with higher VAS scores and stronger desires for urgent intervention for permanent teeth injuries compared with primary teeth injuries which shows that parents still do not care about primary teeth as much as permanent teeth.

Electrospun nanofiber reinforcement of dental composites with electromagnetic alignment approach.

Polymethylmethacrylate (PMMA) is commonly used as a base acrylic denture material with benefits of rapid and easy handling, however, when it is used in prosthetic dentistry, fracturing or cracking problems can be seen due to the relatively low strength issues. Besides, acrylic resin is the still prominent material for denture fabrication due to its handy and low cost features. Numerous proposed fillers that are used to produce PMMA composites, however electrospun polyvinylalcohol (PVA) nanofiber fillers for production of PMMA composite resins are not studied as much as the others. The other focus of the practice is to compare both mechanical properties and efficiency of aligned fibers versus non-aligned PVA nanofibers in PMMA based dental composites. Field-controlled electrospinning system is manufactured and provided good alignment in lab scale as one of contributions. Some novel auxiliary electrodes in controlled structure are augmented to obtain different patterns of alignment with a certain range of fiber diameters. Scanning electron microscopy is used for physical characterization to determine the range of fiber diameters. Non-woven fiber has no unique pattern due to chaotic nature of electrospinning process, but aligned fibers have round pattern or crossed lines. These produced fibers are structured as layer-by-layer form with different features, and these features are used in producing PMMA dental composites with different volume ratios. The maximum flexural strength figure shows that fiber load by weight of 0.25% w/w and above improves in the maximum level. As a result, mechanical properties of PMMA dental composites are improved by using PVA nanofibers as a filler, however the improvement was higher when aligned PVA nanofibers are used. The maximum values were 5.1 MPa (flexural strength), 0.8 GPa (elastic modulus), and 170 kJ/m(3) (toughness) in three-point bending test. In addition to the positive results of aligned and non-aligned nanofibers it was found that they both have a non-toxic feature.