[Alkaloids of Vinca rosea L. introduced to Western Georgia].

Vinca roseae L. (Саtharanthus rosea (L.) G. Don) was introduced at Kobuleti experimental station of medical plants. The object of investigation was the plant material of Vinca roseae L. collected in May, 2005., September, 2006 and October, 2009. Total alkaloids were obtained in accordance with Atta- ur-Rachman method. The variability of the quantitative and qualitative composition of total alkaloids and vincaleikoblastin (VLB) fraction during vegetation was studied. It was established that the maximal content of total alkaloids and VLB fraction of Vinca roseae L. is accumulated in the phase of secondary flowering, hence the collecting of a plant material is recommended to be made during the aforesaid vegetation phase as for this period it is a rather high output of a raw material, alkaloid complex and VLB faction. Alkaloids vinkaleikoblastin, ajmalicine and new epimer tetrahydroalstonine with С3Н-α- orientation were yielded, separated and identified using modern physical-chemical and spectral methods (13С NMR).

[Study on the separation process of pharmacological active total alkaloids from Chelidonium majus L. growing in Georgia].

In present article was studied the separation process of pharmacological active total alkaloids from Chelidonium majus L. growing in Georgia. Alkaloids were extracted from medicinal herbal material and separated by liquid extraction, diluents gas and a microfiltration through membrane equipment. The obtained A1, A2, A3 fractions were analyzed by GC/MS method; in all cases separation proceeds by the principle of extraction of the target alkaloids. It was concluded that the A1 is enriched with α and ß cryptopins, and protopin, but homochelidonine and chelidonine are in low contents. As accompanying alkaloid is identified dihydrosanguinarine as an artifact; the A2 is enriched with the maximum contents of stylopine and protopin, but the poor contents of chelidonine and homochelidonine; the A3 is enriched with α and ß cryptopins and maximum content of chelidonine. Extraction of alkaloids from Chelidonium majus L. proceeds selectively, but depending on a way of separation of the total alkaloids allows varying qualitative and quantitative consistence of the final product.

[Extraction of pharmacologically active alkaloids from Vinca species].

From the Genius Vinca the drugs have been received, arbitrally named: Vingerbin- with anthyarithmic activity, Vincabral-for improvement of brain blood circulation and leicobetin-as stimulator of leicopoesis. The investigations has been performed for creation of rational, resource saving, rentable phyto technologies. As a result, the liquid extraction general schema is provided for receipt of indolic alkaloids from plants V. herbaceae and V.minor. Analyses have shown that extraction with diluent gaz gives the possibility to receive the sums rich with alkaloids:Vingerbin and Vincabral. The advantage of extraction with diluent gaz is exclusion of high volumes of organic and non organic chemicals on the stage of extraction from raw material and liquid extraction, and remain and lipofil fraction converse to new phytosubtances for receipt of biologically active flavonoids, amino acids etc.