Plant elicitation and TiO2 nanoparticles application as an effective strategy for improving the growth, biochemical properties, and essential oil of peppermint.

Mentha piperita L., which is an abundant source of essential oils (EO) and phenolic acids, is well known for its medicinal significance. The present research aimed to evaluate the impact of various concentrations of methyl jasmonate (MeJA; 0, 0.1, and 0.5 mM), titanium dioxide nanoparticles (TiO2 NPs; 0 and 150 mg L-1), and salicylic acid (SA; 0, 0.1, and 1 mM) on growth, EOs, and phenolic compounds of M. piperita L. The results demonstrated that the simultaneous application of SA (0.1 mM) and TiO2 NPs (150 mg L-1) enhanced shoot dry weight, the shoot length, and membrane stability index of peppermint by 56.17, 19.52, and 36%, respectively, compared to control. Moreover, phenolic content (76%), caffeic acid content (78%), rosmarinic acid content (87%), 2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability (78%), and catalase (155%), ascorbate peroxidase activities (95%) were further improved by simultaneously applying MeJA (0.1 mM) and TiO2 NPs (150 mg L-1) compared to control. The highest menthol production (44.51%) was obtained with exogenous application of MeJA (0.1 mM) with 150 mg L-1 TiO2 NPs. The findings of the current study presented an ideal combination of TiO2 NPs with plant growth regulators for promoting antioxidant activities and increasing major components of EO in peppermint plants.

The SP/NK1R System-Mediated ROS Generation in GBM Cells through Inhibiting Glutaredoxin Protein.

Altered redox balance is among the main contributing factors developing glioblastoma multiforme (GBM), a highly aggressive grade IV brain tumor. Neuropeptide substance P (SP) plays a key role in modifying the cellular redox environment by activating the neurokinin-1 receptor (NK1R). In this study, we aimed to investigate the redox-modulating properties of both SP and a commercially available NK1R antagonist, aprepitant in GBM cells. To detect the effect of aprepitant on the viability of U87 glioblastoma cells, resazurin assay was applied. The level of intracellular ROS was assessed using 2',7'-dichlorodihydrofluorescein diacetate (H2DCFDA) assay. The expression of glutaredoxin, a well-known redox-active protein, was measured by quantitative real-time polymerase chain reaction (qRT-PCR). Concurrently, the activity of glutaredoxin was also analyzed by a commercial kit (ZellBio GmbH). We found that SP increased the intracellular levels of reactive oxygen species (ROS) in U87 GBM cells, and aprepitant remarkably decreased this effect. We also explored the effects of SP/NK1R signaling on the glutaredoxin system as a major cellular redox buffer in GBM cells. SP reduced both expression and enzymatic activity of glutaredoxin, and these effects were significantly decreased by aprepitant. In conclusion, our results suggest a possible involvement of SP/NK1R signaling in GBM pathogenesis through oxidative stress and offering new insight for the application of aprepitant as a redox-modulating strategy in GBM patients.