Stalactites Core Prospect as Environmental "Microbial Ark": The Actinomycetota Diversity Paradigm, First Reported from a Greek Cave.

Speleothems found in caves worldwide are considered the natural libraries of paleontology. Bacteria found in these ecosystems are generally limited to Proteobacteria and Actinomycetota, but rare microbiome and "Dark Matter" is generally under-investigated and often neglected. This research article discusses, for the first time to our knowledge, the diachronic diversity of Actinomycetota entrapped inside a cave stalactite. The planet's environmental microbial community profile of different eras can be stored in these refugia (speleothems). These speleothems could be an environmental "Microbial Ark" storing rare microbiome and "Dark Matter" bacterial communities evermore.

ITS Metabarcoding Reveals the Effects of Oregano Essential Oil on Fusarium oxysporum and Other Fungal Species in Soil Samples.

The destructive effects of Fusarium wilts are known to affect the production of many crops. The control of Fusarium oxysporum and other soilborne pathogens was mainly based on soil fumigation (methyl bromide), which has long been prohibited and, nowadays, is based on a limited number of available fungicides due to legislation restrictions on residue tolerances and environmental impacts. Alternatively, natural and environmentally safe compounds, such as essential oils, are being investigated for their efficacy in the control of soilborne diseases. The great fungicidal ability of the oregano essential oil components (carvacrol and thymol) has been reported to inhibit the germination and the mycelial development of several fungal species, including F. oxysporum. The aim of our study was to demonstrate how the metabarcoding approach can provide valuable information about the positive or negative impacts of two different doses of oregano essential oil on Fusarium oxysporum and other fungal species which were present in the studied soil samples through the amplification of the ITS1 and ITS2 regions, which were analyzed on a MiSeq platform. A higher dose of oregano essential oil decreased the abundance of F. oxysporum, along with other fungal species, but also had negative effects on Trichoderma evansii and Mortierella chlamydospora, species with possible fungicidal properties. Soil properties, essential oil properties, the fungal composition, and interactions between fungal species should be considered as factors influencing the effectiveness of essential oils as biological control agents for soilborne pathogens.

Metataxonomic Analysis of Bacteria Entrapped in a Stalactite's Core and Their Possible Environmental Origins.

Much is known about microbes originally identified in caves, but little is known about the entrapment of microbes (bacteria) in stalactites and their possible environmental origins. This study presents data regarding the significant environmental distribution of prokaryotic bacterial taxa of a Greek stalactite core. We investigated the involvement of those bacteria communities in stalactites using a metataxonomic analysis approach of partial 16S rRNA genes. The metataxonomic analysis of stalactite core material revealed an exceptionally broad ecological spectrum of bacteria classified as members of Proteobacteria, Actinobacteria, Firmicutes, Verrucomicrobia, and other unclassified bacteria. We concluded that (i) the bacterial transport process is possible through water movement from the upper ground cave environment, forming cave speleothems such as stalactites, (ii) bacterial genera such as Polaromonas, Thioprofundum, and phylum Verrucomicrobia trapped inside the stalactite support the paleoecology, paleomicrobiology, and paleoclimate variations, (iii) the entrapment of certain bacteria taxa associated with water, soil, animals, and plants such as Micrococcales, Propionibacteriales, Acidimicrobiales, Pseudonocardiales, and α-, ß-, and γ-Proteobacteria.

Comparison of neem or oregano with thiram on organic matter decomposition of a sand loam soil amended with compost, and on soil biological activity.

BACKGROUND: Oregano essential oil and neem have been reported to be effective against soil-borne pathogens and nematodes. The possibility of having an effect on soil properties was the aim of this investigation. Moreover, thiram, a common widely used chemical pesticide, was used for comparison. RESULTS: The effects of all three above-mentioned substances were investigated during an incubation experiment, for 15 weeks. Crushed neem pellets were added at 1.0, 2.0 or 3.0 g per 50 g of soil. Oregano dry matter was applied at 0.2, 0.4 or 0.6 g per 50 g of soil. Finally, thiram was applied at 0.1, 0.2 and 0.3 g per 50 g of soil. The addition of neem resulted in a decrease in organic carbon mineralisation, higher than that of oregano, but not as prominently as thiram. The addition of neem resulted in an increase in the content of nitrate, organic P, bioavailable P, bioavailable K and bioavailable Mn. Oregano had a slight negative effect on organic matter biodegradation, but caused an increase of nutritional mineral elements. Microbe colonies in soil were increased by the addition of neem cake granules, whereas oregano and thiram treatments caused a decrease. The addition of thiram reduced nitrate and available Mn contents, but at the lowest dose increased organic P and available K. Thiram reduced prominently available Cu at the upper rates, but it increased these forms at the lowest rate, while available Zn content was increased in two lowest rates. CONCLUSION: The results of this study indicated that neem or oregano could be applied to the soil without any extremely negative effect on the available forms of nutritional mineral elements, as thiram does.