Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 20 de 40
Filtrar
11.
Ann Dermatol Venereol ; 143(11): 675-681, 2016 Nov.
Artigo em Francês | MEDLINE | ID: mdl-27659388

RESUMO

BACKGROUND: Since 2007 in France, human papilloma virus (HPV) vaccination has been licensed for use as a vaccine against HPV 6, 11, 16 and 18. The impact on the epidemiology of external genital warts (EGWs) in a large population remains unclear. OBJECTIVES: To determine epidemiologic and clinical features of patients presenting EGWs in France in the era of HPV vaccination. PATIENTS AND METHODS: In this prospective, observational study, we analyzed clinical features and treatments between January 1st, 2012 and March 31, 2012 for patients consulting for EGWs at 15 STI clinics throughout France. RESULTS: A total of 372 men and 111 women were included; mean age 31.2 years. The women were younger than the men (31.7 and 28.9 years respectively P<0.05). Among the patients, 416 (85.7%) were heterosexual, 13 bisexual and 54 (11.2%) homosexual, including one female. Males reported more sexual partners in the last 12 months (more than 3 partners in 32.6% versus 11.9%, P<0.01). Among the men, 230 had involvement of the penis alone and 46 had involvement of the anus alone. Seventy-six patients had EGWs of the anus, and of these 26 were MSM. In females, 76 had an infection of the vulva alone and 22 co-infection of the vulva and anus. MSM and females were at higher risk than heterosexual males for anal involvement (P<0.0001 and P=0.004, respectively). Three women had been vaccinated: two with Gardasil® and one with Cervarix®. Cryotherapy was the preferred treatment. CONCLUSION: With the advent of HPV vaccination, a global strategy for the prevention and treatment of EGW should be implemented.


Assuntos
Doenças do Ânus/epidemiologia , Condiloma Acuminado/epidemiologia , Doenças dos Genitais Femininos/epidemiologia , Doenças dos Genitais Masculinos/epidemiologia , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Doenças do Ânus/terapia , Condiloma Acuminado/terapia , Crioterapia , Feminino , França/epidemiologia , Doenças dos Genitais Femininos/terapia , Doenças dos Genitais Masculinos/terapia , Vacina Quadrivalente Recombinante contra HPV tipos 6, 11, 16, 18 , Humanos , Masculino , Pessoa de Meia-Idade , Estudos Prospectivos , Parceiros Sexuais , Sexualidade/estatística & dados numéricos , Adulto Jovem
12.
Int J Obes (Lond) ; 38(1): 148-51, 2014 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-23649472

RESUMO

The central melanocortin system is essential for the regulation of long-term energy homeostasis in humans. Rodent experiments suggest that this system also affects glucose metabolism, in particular by modulating peripheral insulin sensitivity independently of its effect on adiposity. Rare patients with complete genetic defects in the central melanocortin system can provide insight into the role of this system in glucose homeostasis in humans. We here describe the eighth individual with complete proopiomelanocortin (POMC) deficiency and the first with coincidental concomitant type 1 diabetes, which provides a unique opportunity to determine the role of melanocortins in glucose homeostasis in human. Direct sequencing of the POMC gene in this severely obese patient with isolated adrenocorticotropic hormone deficiency identified a homozygous 5' untranslated region mutation -11C>A, which we find to abolish normal POMC protein synthesis, as assessed in vitro. The patient's insulin requirements were as expected for his age and pubertal development. This unique patient suggests that in humans the central melanocortin system does not seem to affect peripheral insulin sensitivity, independently of its effect on adiposity.


Assuntos
Insuficiência Adrenal/genética , Diabetes Mellitus Tipo 1/genética , Resistência à Insulina/genética , Melanocortinas/metabolismo , Obesidade/genética , Obesidade Infantil/genética , Pró-Opiomelanocortina/deficiência , Insuficiência Adrenal/complicações , Criança , Metabolismo Energético , Comportamento Alimentar , Genótipo , Homeostase , Humanos , Masculino , Melanocortinas/genética , Obesidade/complicações , Obesidade Infantil/etiologia , Pró-Opiomelanocortina/genética , Análise de Sequência de DNA , Aumento de Peso/genética
13.
Osteoporos Int ; 22(8): 2283-93, 2011 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-20941479

RESUMO

UNLABELLED: Diabetic obesity is associated with increased fracture risk in adults and adolescents. We find in both adolescent and adult mice dramatically inferior mechanical properties and structural quality of cortical bone, in agreement with the human fracture data, although some aspects of the response to obesity appear to differ by age. INTRODUCTION: The association of obesity with bone is complex and varies with age. Diabetic obese adolescents and adult humans have increased fracture risk. Prior studies have shown reduced mechanical properties as a result of high-fat diet (HFD) but do not fully address size-independent mechanical properties or structural quality, which are important to understand material behavior. METHODS: Cortical bone from femurs and tibiae from two age groups of C57BL/6 mice fed either HFD or low-fat diet (LFD) were evaluated for structural and bone turnover changes (SEM and histomorphometry) and tested for bending strength, bending stiffness, and fracture toughness. Leptin, IGF-I, and non-enzymatic glycation measurements were also collected. RESULTS: In both young and adult mice fed on HFD, femoral strength, stiffness, and toughness are all dramatically lower than controls. Inferior lamellar and osteocyte alignment also point to reduced structural quality in both age groups. Bone size was largely unaffected by HFD, although there was a shift from increasing bone size in obese adolescents to decreasing in adults. IGF-I levels were lower in young obese mice only. CONCLUSIONS: While the response to obesity of murine cortical bone mass, bone formation, and hormonal changes appear to differ by age, the bone mechanical properties for young and adult groups are similar. In agreement with human fracture trends, adult mice may be similarly susceptible to bone fracture to the young group, although cortical bone in the two age groups responds to diabetic obesity differently.


Assuntos
Envelhecimento , Osso e Ossos/fisiopatologia , Dieta Hiperlipídica/efeitos adversos , Obesidade/fisiopatologia , Envelhecimento/patologia , Envelhecimento/fisiologia , Animais , Fenômenos Biomecânicos , Glicemia/metabolismo , Composição Corporal , Densidade Óssea/fisiologia , Osso e Ossos/patologia , Diabetes Mellitus Experimental/sangue , Diabetes Mellitus Experimental/complicações , Diabetes Mellitus Experimental/patologia , Diabetes Mellitus Experimental/fisiopatologia , Fêmur/fisiopatologia , Fêmur/ultraestrutura , Produtos Finais de Glicação Avançada/sangue , Fator de Crescimento Insulin-Like I/metabolismo , Leptina/sangue , Masculino , Camundongos , Camundongos Endogâmicos C57BL , Microscopia Eletrônica de Varredura , Obesidade/sangue , Obesidade/patologia , Fraturas por Osteoporose/etiologia , Fraturas por Osteoporose/patologia , Fraturas por Osteoporose/fisiopatologia , Tíbia/fisiopatologia , Tíbia/ultraestrutura , Aumento de Peso/fisiologia
14.
Int J Obes (Lond) ; 35(3): 457-61, 2011 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-20733581

RESUMO

Bariatric surgery is often successful for treatment of severe obesity. The mechanisms of weight loss after bariatric surgery and the role of central energy homeostatic pathways in this weight loss process are not well understood. The study of individuals with complete loss of function of genes important in the leptin-melanocortin system may help establish the significance of these pathways for weight loss after bariatric surgery. We describe the outcome of bariatric surgery in an adolescent with compound heterozygosity and complete functional loss of both alleles of the melanocortin 4 receptor (MC4R). The patient underwent laparoscopic adjustable gastric banding and truncal vagotomy at years of age, which resulted in initial, but not long-term weight loss. Our experience with this patient suggests that complete MC4R deficiency impairs response to gastric banding and results in poor weight loss after this surgery.


Assuntos
Cirurgia Bariátrica/métodos , Obesidade Mórbida/cirurgia , Receptor Tipo 4 de Melanocortina/deficiência , Redução de Peso/fisiologia , Adolescente , Humanos , Masculino , Obesidade Mórbida/genética , Resultado do Tratamento , Redução de Peso/genética
15.
Bone ; 46(1): 217-25, 2010 Jan.
Artigo em Inglês | MEDLINE | ID: mdl-19853069

RESUMO

Overweight and obesity are rapidly expanding health problems in children and adolescents. Obesity is associated with greater bone mineral content that might be expected to protect against fracture, which has been observed in adults. Paradoxically, however, the incidence of bone fractures has been found to increase in overweight and obese children and adolescents. Prior studies have shown some reduced mechanical properties as a result of high-fat diet (HFD) but do not fully address size-independent measures of mechanical properties, which are important to understand material behavior. To clarify the effects of HFD on the mechanical properties and microstructure of bone, femora from C57BL/6 mice fed either a HFD or standard laboratory chow (Chow) were evaluated for structural changes and tested for bending strength, bending stiffness and fracture toughness. Here, we find that in young, obese, high-fat fed mice, all geometric parameters of the femoral bone, except length, are increased, but strength, bending stiffness, and fracture toughness are all reduced. This increased bone size and reduced size-independent mechanical properties suggests that obesity leads to a general reduction in bone quality despite an increase in bone quantity; yield and maximum loads, however, remained unchanged, suggesting compensatory mechanisms. We conclude that diet-induced obesity increases bone size and reduces size-independent mechanical properties of cortical bone in mice. This study indicates that bone quantity and bone quality play important compensatory roles in determining fracture risk.


Assuntos
Osso e Ossos/patologia , Dieta , Gorduras na Dieta/efeitos adversos , Obesidade/induzido quimicamente , Obesidade/patologia , Animais , Fenômenos Biomecânicos , Composição Corporal , Densidade Óssea , Osso e Ossos/metabolismo , Osso e Ossos/fisiopatologia , Modelos Animais de Doenças , Teste de Tolerância a Glucose , Fator de Crescimento Insulin-Like I/metabolismo , Leptina/sangue , Masculino , Camundongos , Microscopia Eletrônica de Varredura , Microscopia Eletrônica de Transmissão , Obesidade/metabolismo , Tomografia Computadorizada por Raios X
16.
Diabetes Obes Metab ; 10(10): 912-20, 2008 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-18093211

RESUMO

Ketosis-prone diabetes (KPD) is a phenotypically defined form of diabetes characterized by male predominance and severe insulin deficiency. Neurogenin3 (NGN3) is a proendocrine gene, which is essential for the fate of pancreatic beta cells. Mice lacking ngn3 develop early insulin-deficient diabetes. Thus, we hypothesized that gender and variants in NGN3 could predispose to KPD. We have studied clinical and metabolic parameters according to gender in patients with KPD (n = 152) and common type 2 diabetes (T2DM) (n = 167). We have sequenced NGN3 in KPD patients and screened gene variants in T2DM and controls (n = 232). In KPD, male gender was associated with a more pronounced decrease in beta-cell insulin secretory reserve, assessed by fasting C-peptide [mean (ng/ml) +/- s.d., M: 1.1 +/- 0.6, F: 1.5 +/- 0.9; p = 0.02] and glucagon-stimulated C-peptide [mean (ng/ml) +/- s.d., M: 2.2 +/- 1.1, F: 3.1 +/- 1.7; p = 0.03]. The rare affected females were in an anovulatory state. We found two new variants in the promoter [-3812T/C (af: 2%) and -3642T/C (af: 1%)], two new coding variants [S171T (af: 1%) and A185S (af: 1%)] and the variant already described [S199F (af: 69%)]. These variants were not associated with diabetes. Clinical investigation revealed an association between 199F and hyperglycaemia assessed by glycated haemoglobin [HbA1c (%, +/-s.d.) S199: 12.6 +/- 1.6, S199F: 12.4 +/- 1.4 and 199F: 14.1 +/- 2.2; p = 0.01]. In vitro, the P171T, A185S and S199F variants did not reveal major functional alteration in the activation of NGN3 target genes. In conclusion, male gender, anovulatory state in females and NGN3 variations may influence the pathogenesis of KPD in West Africans. This has therapeutic implications for potential tailored pharmacological intervention in this population.


Assuntos
Fatores de Transcrição Hélice-Alça-Hélice Básicos/genética , Diabetes Mellitus Tipo 2/etiologia , Cetoacidose Diabética/etiologia , Proteínas do Tecido Nervoso/genética , Regiões Promotoras Genéticas , Fatores Sexuais , Adulto , Anovulação , Biomarcadores/sangue , População Negra/genética , Peptídeo C/análise , Estudos de Casos e Controles , Distribuição de Qui-Quadrado , Diabetes Mellitus Tipo 2/sangue , Diabetes Mellitus Tipo 2/etnologia , Cetoacidose Diabética/sangue , Cetoacidose Diabética/etnologia , Feminino , Expressão Gênica , Genótipo , Glucagon , Humanos , Insulina/metabolismo , Secreção de Insulina , Masculino , Pessoa de Meia-Idade
17.
Diabetes ; 49(8): 1347-52, 2000 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-10923636

RESUMO

Leptin resistance and obesity have been related to mutations of the leptin receptor gene in rodents and, recently, in a consanguineous family. The latter mutation results in a receptor lacking transmembrane and intracellular domains. Homozygous and heterozygous individuals with this mutation had serum leptin levels higher than expected, given their BMIs: 600, 670, and 526 ng/ml and 145, 362, 294, 240, and 212 ng/ml, respectively. Their serum leptin was fractionated by gel filtration: >80% was present as a high-molecular size complex vs. 7.5% in the nonmutated sister. Western blot analysis showed a band at 146 kDa reacting specifically with an antibody directed against the leptin receptor ectodomain. In 10 obese control subjects, as in the mutated patients, free leptin levels correlated with BMI (r = 0.70, P = 0.0011) and reflected fat mass, regardless of leptin receptor functioning. In the patients, bound leptin levels correlated with BMI (r = 0.99, P = 0.0002) and were related to the number of mutated alleles. These data demonstrate that the truncated receptor is secreted into blood and binds the majority of serum leptin, markedly increasing bound and total leptin. Free serum leptin was similarly correlated with BMI in the mutated and nonmutated obese individuals, providing evidence that the relationship between BMI and circulating free leptin is preserved in this family. This finding suggests that the leptin receptor itself may not be specifically involved in the control of leptin secretion, and it supports the concept of relative resistance to leptin in common obesity.


Assuntos
Tecido Adiposo/anatomia & histologia , Proteínas de Transporte/sangue , Proteínas de Transporte/genética , Resistência a Medicamentos , Leptina/farmacologia , Obesidade/sangue , Receptores de Superfície Celular , Adolescente , Adulto , Biomarcadores/sangue , Criança , Cromatografia em Gel , Consanguinidade , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Peso Molecular , Mutação , Obesidade/genética , Receptores para Leptina , Valores de Referência , Análise de Regressão
18.
Hum Hered ; 50(6): 370-81, 2000.
Artigo em Inglês | MEDLINE | ID: mdl-10899756

RESUMO

Hepatocyte nuclear factors 3 (HNF-3 alpha, -3 beta and -3 gamma) belong to an evolutionarily conserved family of transcription factors that are critical for diverse biological processes such as development, differentiation and metabolism. Gene expression studies have shown that HNF3 proteins are critical regulators of the early-onset type 2 diabetes genes HNF-1 alpha, HNF-4 alpha and IPF-1/PDX-1 (MODY3, 1 and 4, respectively) and of glucagon transcription and pancreatic alpha-cell function. In this study, we investigated whether genetic variation in the genes encoding HNF-3 alpha, HNF-3 beta and HNF-3 gamma predisposes humans to hyperglycemic or hypoglycemic syndromes. In addition, we report the cloning and partial nucleotide sequence of the human HNF-3 alpha, -3 beta and -3 gamma genes. Mutation screening included 96 subjects with type 2 diabetes mellitus, as well as one family with persistent neonatal hypoglycemia. No functional mutations were detected in the coding sequences of the three HNF-3 genes. Our results suggest that mutations in HNF-3 genes are not a common cause of type 2 diabetes mellitus. The data provided will facilitate genetic studies in other populations and will advance our understanding of the role HNF-3 plays in the development of diabetes mellitus and other metabolic disorders of glucose homeostasis.


Assuntos
Proteínas de Ligação a DNA/genética , Diabetes Mellitus Tipo 2/genética , Glucose/metabolismo , Homeostase/genética , Proteínas Nucleares/genética , Fatores de Transcrição/genética , Adulto , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA , Primers do DNA , Proteínas de Ligação a DNA/fisiologia , Variação Genética , Humanos , Hipoglicemia/genética , Recém-Nascido , Masculino , Dados de Sequência Molecular , Mutação de Sentido Incorreto , Proteínas Nucleares/fisiologia , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/fisiologia , Ativação Transcricional/fisiologia
19.
J Clin Invest ; 106(2): 253-62, 2000 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-10903341

RESUMO

By integrating an agonist satiety signal, provided by alpha-melanocyte-stimulating hormone (alpha-MSH), and an antagonist signal, provided by agouti-related protein (AGRP), the melanocortin-4 receptor (MC4-R) is a key element in the hypothalamic control of food intake. Inactivation of the gene encoding this G protein-coupled receptor causes obesity in mice. In humans, frameshift mutations in MC4-R cause an early-onset dominant form of obesity in two families. In this study we find a high frequency (4%) of rare heterozygous MC4-R mutations in a large population of morbidly obese patients. No such mutations were found in controls. By analyzing the phenotypes of the probands carrying these mutations, we demonstrate that these patients display a common, nonsyndromic form of obesity. Interestingly, functional analysis of the mutant receptors indicates that obesity-associated defects in MC4-R range from loss of function to constitutive activation. Transmission of these mutations in the families of the carriers indicates a variable expressivity that is not related to the functional severity of the mutations. This variable expressivity of MC4-R-associated obesity is not due to variations in genes for alpha-MSH or AGRP. Taken together, these results demonstrate that MC4-R mutations are a frequent but heterogeneous genetic cause of morbid obesity.


Assuntos
Mutação , Obesidade Mórbida/genética , Receptores da Corticotropina/genética , Adolescente , Adulto , Idoso , Idoso de 80 Anos ou mais , Proteína Relacionada com Agouti , Criança , Estudos de Coortes , Ingestão de Alimentos , Feminino , Mutação da Fase de Leitura , Testes Genéticos , Heterozigoto , Humanos , Peptídeos e Proteínas de Sinalização Intercelular , Masculino , Pessoa de Meia-Idade , Mutação de Sentido Incorreto , Linhagem , Penetrância , Proteínas/metabolismo , Receptor Tipo 4 de Melanocortina , alfa-MSH/metabolismo
20.
Int J Obes Relat Metab Disord ; 24(3): 391-3, 2000 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-10757637

RESUMO

OBJECTIVE: Peroxisome-proliferator-activated receptors gamma (PPAR gamma), is a key regulator of adipocyte differentiation and energy balance. Two naturally occurring mutations in the PPAR gamma gene, Pro115Gln and Pro12Ala, have recently been shown to impair the function of the PPAR gamma2 isoform of the receptor and to be associated with obesity or diabetes-related phenotypes in different populations. SUBJECTS: We studied the occurrence and possible associations of the Pro115Gln and Pro12Ala in the PPAR gamma2 gene with several clinical and metabolic phenotypes in three independent large populations of non-obese non-diabetic, type 2 diabetic, and morbidly obese French Caucasians. RESULTS: The Pro115Gln mutation was not found in any of the 1069 subjects screened including 626 obese patients. The frequency of the Pro12Ala mutation was similar in all groups (0.08, 0.11, 0.09) and was not associated with BMI or any of the clinical parameters tested. CONCLUSIONS: We conclude that the Pro115Gln mutation is not a frequent cause of morbid obesity in Caucasians and that the Pro12Ala mutation is not associated with clinically significant changes in these populations.


Assuntos
Diabetes Mellitus Tipo 2/genética , Mutação , Obesidade Mórbida/genética , Receptores Citoplasmáticos e Nucleares/genética , Fatores de Transcrição/genética , Adulto , Idoso , Alanina , Feminino , Genótipo , Glutamina , Humanos , Masculino , Pessoa de Meia-Idade , Prolina
SELEÇÃO DE REFERÊNCIAS
DETALHE DA PESQUISA