Water footprint of lemon production in Argentina.

This work comes to fill a knowledge gap regarding the sustainability of the lemon industry. The motivation is the prominent role of the citrus sector in the economy of the northwest of Argentina and in the world: Argentina is the eighth largest producer of fresh citrus fruits, one of the four leading lemon-producing countries and the world's first lemon processor. A water footprint study is conducted to obtain relevant information on the water consumption and degradation associated with citrus production, according to the Water Footprint Network guidelines. A remarkable aspect is that most data used come mainly from actual practices and sources considering daily weather data of seven weather stations from 2012 to 2018. The green, blue and grey water footprints linked to the primary production of lemons are evaluated every year. The water footprint is distributed approximately in 69% (234 m3t-1) green, 8% (30 m3t-1) blue and 23% (82 m3t-1) grey. In addition, the Available Water Remaining (AWARE) indicator is used for outlining the blue water sustainability, resulting in a potential for blue water deprivation of 102 m3 world eq. per tonne of harvested lemons. This work is expected to serve as a baseline to provide diagnosis and improvement opportunities in the lemon sector for public and private stakeholders.

Synergistic Effect of Simple Sugars and Carboxymethyl Cellulose on the Production of a Cellulolytic Cocktail from Bacillus sp. AR03 and Enzyme Activity Characterization.

A cellulase-producing bacterium isolated from pulp and paper feedstock, Bacillus sp. AR03, was evaluated by means of a factorial design showing that peptone and carbohydrates were the main variables affecting enzyme production. Simple sugars, individually and combined with carboxymethyl cellulose (CMC), were further examined for their influence on cellulase production by strain AR03. Most of the mono and disaccharides assayed presented a synergistic effect with CMC. As a result, a peptone-based broth supplemented with 10 g/L sucrose and 10 g/L CMC maximized enzyme production after 96 h of cultivation. This medium was used to produce endoglucanases in a 1-L stirred tank reactor in batch mode at 30 °C, which reduced the fermentation period to 48 h and reaching 3.12 ± 0.02 IU/mL of enzyme activity. Bacillus sp. AR03 endoglucanases showed an optimum temperature of 60 °C and a pH of 6.0 with a wide range of pH stability. Furthermore, presence of 10 mM Mn(2+) and 5 mM Co(2+) produced an increase of enzyme activity (246.7 and 183.7 %, respectively), and remarkable tolerance to NaCl, Tween 80, and EDTA was also observed. According to our results, the properties of the cellulolytic cocktail from Bacillus sp. AR03 offer promising features in view of potential biorefinery applications.