RESUMO
A new antibacterial antibiotic complex, aridicin, was produced by a new genus, Kibdelosporangium aridum (SK&F-AAD-216). The individual factors, aridicins A, B and C, were isolated from the fermentation broth by an Amberlite XAD-7 resin extraction and purified by preparative reversed phase HPLC. The aridicins were found to be novel members of the glycopeptide class of antibiotics as exemplified by ristocetin and vancomycin, based on chemical and spectroscopic data, their molecular weights as determined by FAB mass spectrometry (1,786, 1,800 and 1,814), the detection of actinoidinic acid in their acid hydrolysates, and detailed TLC and HPLC comparisons with representative members of this class.
Assuntos
Actinomycetales/metabolismo , Antibacterianos , Antibacterianos/isolamento & purificação , Aminoácidos/análise , Antibacterianos/análise , Cromatografia Líquida de Alta Pressão , Glicopeptídeos/análise , Glicopeptídeos/isolamento & purificação , Peso Molecular , Conformação Proteica , Vancomicina/análiseRESUMO
Transmission (TEM) and scanning electron microscopy (SEM) of Candida albicans cultures treated with the cell wall active antibiotics aculeacin A and papulacandin B (10 micrograms/mL) revealed highly distorted, wrinkled, and collapsed cells. Dividing cells failed to separate properly and aggregates of enlarged and elongated forms were often seen. TEM sections revealed thick and layered cell walls in the treated cultures and bud cross walls failed to segregate completely. Approximately 20% of the cells demonstrated complete cell necrosis accompanied with cytoplasmic deterioration, layered and distorted walls, and improperly formed buds and scars.
Assuntos
Aminoglicosídeos , Antibacterianos , Antifúngicos/farmacologia , Candida albicans/efeitos dos fármacos , Peptídeos Cíclicos , Candida albicans/ultraestrutura , Parede Celular/efeitos dos fármacos , Parede Celular/ultraestrutura , Glicosídeos/farmacologia , Microscopia Eletrônica , Microscopia Eletrônica de VarreduraRESUMO
The anthelmintic agent methyl 5(6)-butyl-2-benzimidazolecarbamate (Parbendazole) was transformed to two of its animal metabolites, methyl 5(6)-(4-hydroxybutyl)-2-benzimidazolecarbamate and methyl 5(6)-3-(carboxypropyl)-2-benzimidazolecarbamate, by the filamentous fungus Cunninghamella bainieri ATCC 9244. The transformation pathway was shown to be through the 4-hydroxybutyl product to the 3-carboxypropyl product. The reaction favored accumulation of the latter product.