RESUMO
As part of the European Scientists Sequencing Arabidopsis program, a contiguous region (396607 bp) located on chromosome 4 around the APETALA2 gene was sequenced. Analysis of the sequence and comparison to public databases predicts 103 genes in this area, which represents a gene density of one gene per 3.85 kb. Almost half of the genes show no significant homology to known database entries. In addition, the first 45 kb of the contig, which covers 11 genes, is similar to a region on chromosome 2, as far as coding sequences are concerned. This observation indicates that ancient duplications of large pieces of DNA have occurred in Arabidopsis.
Assuntos
Duplicação Gênica , Genes de Plantas , Proteínas de Homeodomínio/genética , Proteínas Nucleares/genética , Proteínas de Plantas/genética , Arabidopsis/genética , Proteínas de Arabidopsis , Sequência de Bases , Mapeamento Cromossômico , Mapeamento de Sequências Contíguas , DNA de Plantas , Genoma de Planta , Íntrons , Computação Matemática , Dados de Sequência Molecular , Família MultigênicaRESUMO
As a contribution to the European Scientists Sequencing Arabidopsis (BIOTECH ESSA) project, a contig of almost 40kb has been sequenced at the extreme top of chromosome 1, around the Arabidopsis thaliana gene coding for a member of the 1-aminocyclopropane-1-carboxylate synthesis gene family. The region contains, besides the ACS1 gene itself, 10 putative genes, all new for Arabidopsis. Among these are three genes encoding kinases, a late embryogenesis-abundant protein, a MADS box-containing protein, a dehydrogenase, and a Myb-related transcription factor. In addition, six cDNAs have been sequenced that correspond to this region.
Assuntos
Arabidopsis/genética , Cromossomos/genética , DNA de Plantas/genética , Proteínas Proto-Oncogênicas c-myb , Arabidopsis/química , Proteínas de Arabidopsis , Mapeamento Cromossômico , Clonagem Molecular , DNA de Plantas/química , Proteínas de Ligação a DNA/genética , Expressão Gênica/genética , Genes de Plantas/genética , Genoma de Planta , Proteínas de Domínio MADS , Dados de Sequência Molecular , Oxirredutases/genética , Fosfotransferases/genética , Proteínas de Plantas/genética , Análise de Sequência de DNA , Homologia de Sequência de Aminoácidos , Fatores de Transcrição/genéticaRESUMO
As part of the European Union program of European Scientist Sequencing Arabidopsis (ESSA), the DNA sequence of a 24.053-bp insert of cosmid clone CC17J13 was determined. The cosmid is located on chromosome 1 at the PFL locus (position 30 cM). Analysis of the sequence and comparison to public databases predicts seven genes in this area, thus approximately one gene every 3.3 kb. Three cDNAs corresponding to genes in this region were also sequenced. The homologies and/or possible functions of the (putative) genes are discussed. Proteins encoded by genes in this region include a polyadenylate-binding protein (PAB-3) and a GTP-binding protein (Rab7) as well as a novel protein, possibly involved in double-stranded RNA unwinding and apoptosis. Intriguingly, the gene encoding the PAB-3 protein, which is very specifically expressed, is flanked by putative matrix attachment regions.
