RESUMO
Bacteria are frequently used in industrial processes and nutrient supplementation to restore a healthy human microflora, but use of live bacteria is often troublesome. Here, we hypothesize that bacterially-derived carbon-quantum-dots obtained through pyrolytic carbonization inherit physico-chemical properties from probiotic and pathogenic source-bacteria. Carbon-quantum-dots carbonized at reaction-temperatures below 200 â°C had negligible quantum-yields, while temperatures above 220 â°C yielded poor water-suspendability. Fourier-transform infrared-spectroscopy demonstrated preservation of amide absorption bands in carbon-quantum-dots derived at intermediate temperatures. X-ray photoelectron-spectroscopy indicated that the at%N in carbon-quantum-dots increased with increasing amounts of protein in source-bacterial surfaces. Carbonization transformed hydrocarbon-like bacterial surface compounds into heterocyclic aromatic-carbon structures, evidenced by a broad infrared absorption band (920-900 âcm-1) and the presence of carbon in C-C functionalities of carbon-quantum-dots. The chemical composition of bacterially-derived carbon-quantum-dots could be explained by the degradation temperatures of main bacterial cell surface compounds. All carbon-quantum-dots generated reactive-oxygen-species, most notably those derived from probiotic lactobacilli, carrying a high amount of surface protein. Concluding, amide functionalities in carbon-quantum-dots are inherited from surface proteins of source-bacteria, controlling reactive-oxygen-species generation. This paves the way for applications of bacterially-derived carbon-quantum-dots in which reactive-oxygen-species generation is essential, instead of hard-to-use live bacteria, such as in food supplementation or probiotic-assisted antibiotic therapy.
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The orthopaedic and trauma community have faced the threat of infection since the introduction of operative fracture fixation many decades ago. The parallel emergence and spread of antimicrobial resistance in clinically relevant pathogens has the potential to significantly complicate patient care. This editorial serves to provide a global context to the issue of antimicrobial resistance and how infectious disease research in general plays a crucial role both on a global scale as evidenced by the current pandemic, but also on a more personal scale for the daily management of orthopaedic trauma patients. The special issue on Orthopaedic Infection in the eCM journal provides a snapshot of the clinically relevant basic research that is being performed in this field.
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Ortopedia , Pandemias , Fixação de Fratura , HumanosRESUMO
Bacterial contamination is hard to avoid during dental implant surgery. Macrophages and their polarisation play a decisive role in bacterial colonisation and tissue integration on bacterially contaminated dental implants. The present study investigated the role of macrophages in stimulating tissue coverage overgrowth of contaminating oral bacteria on polished titanium (Ti-P) and acid-etched zirconium dioxide (ZrO2-MA) dental implant materials. Different co-culture models were employed to determine phagocytosis rates of Streptococcus mitis or Staphylococcus aureus contaminating a dental implant surface and the influence of contaminating bacteria and osteoblasts (U2OS) on macrophage polarisation. S. aureus was phagocytized in higher numbers than S. mitis in bi-cultures on smooth Ti-P surfaces. Contaminating S. mitis stimulated near full polarisation of macrophages from a non-Ym1-expressing- to a Ym1-expressing-phenotype on smooth Ti-P, but on ZrO2-MA both phenotypes occurred. In tri-cultures with U2OS-cells on smooth Ti-P, a larger percentage of macrophages remained in their non-Ym1-expressing, "fighting" M1-like phenotype to clear Ti-P surfaces from contaminating bacteria. On ZrO2-MA surfaces, more macrophages tended towards their "fix- and-repair" M2-like phenotype than on Ti-P surfaces. Surface coverage of smooth, bacterially contaminated Ti-P surfaces by U2OS-cells was more effectively stimulated by fighting, M1-like macrophages than on ZrO2-MA surfaces. Comprehensive guidelines are provided for the development of infection-resistant, dental implant materials, including bacteria, tissue and immune cells. These guidelines point to more promising results for clinical application of Ti-P as compared with ZrO2-MA.
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Implantes Dentários/microbiologia , Macrófagos/fisiologia , Fagocitose/fisiologia , Animais , Biofilmes/crescimento & desenvolvimento , Humanos , Ativação de Macrófagos/fisiologia , Macrófagos/microbiologia , Camundongos , Osteoblastos/efeitos dos fármacos , Osteoblastos/microbiologia , Fagocitose/efeitos dos fármacos , Staphylococcus aureus/crescimento & desenvolvimento , Streptococcus mitis/crescimento & desenvolvimento , Propriedades de Superfície , Titânio/farmacologia , Zircônio/farmacologiaRESUMO
OBJECTIVES: Evaluation of the influence of a smooth surface moulding technique of silicone rubber indwelling voice prostheses on in vitro biofilm formation and analysis of the clinical in situ lifetime. DESIGN: Biofilm formation on smooth and Groningen ultra low resistance (URL) prostheses was studied in an artificial throat model. The clinical lifetime of smooth voice prostheses was compared to the previous lifetime of URL by counting the number of replacements in a consecutive 6-month period in the same patient. PARTICIPANTS: Eleven laryngectomised patients in follow-up who required frequent replacement of their voice prostheses. SETTINGS: Tertiary University Medical Center. RESULTS: Use of a smoother mould and less viscous silicone rubber yielded a decrease in surface roughness from 46 to 8 nm and was accompanied by a 40% reduction in the prevalence of bacteria and yeast in in vitro formed biofilms. Clinically, the lifetime was significantly (P<.005) increased by a factor of 2.1. CONCLUSIONS: This combined in vitro and clinical study suggests that the choice of material and in particular its surface finishing may be determining factors with respect to the clinical lifetime of silicone rubber implants and devices failing due to biofilm formation.
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Biofilmes , Laringe Artificial , Desenho de Prótese , Elastômeros de Silicone , Idoso , Idoso de 80 Anos ou mais , Glote , Humanos , Neoplasias Hipofaríngeas/cirurgia , Neoplasias Laríngeas/cirurgia , Laringectomia , Masculino , Pessoa de Meia-Idade , Falha de Prótese , Propriedades de SuperfícieRESUMO
Oral biofilm can never be fully removed by oral hygiene measures. Biofilm left behind after brushing is often left behind on the same sites and exposed multiple times to antimicrobials from toothpastes and mouthrinses, after which removal becomes increasingly difficult. On the basis of this observation, we hypothesize that oral bacteria adhering to salivary conditioning films become more difficult to remove after adsorption of antimicrobials due to stiffening of their adhesive bond. To verify this hypothesis, bacteria adhering to bare and saliva-coated glass were exposed to 3 different mouthrinses, containing chlorhexidine-digluconate, cetylpyridinium-chloride, or amine-fluoride, after which bacterial vibration spectroscopy was carried out or a liquid-air interface was passed over the adhering bacteria to stimulate their detachment. Brownian motion-induced nanoscopic vibration amplitudes of 4 oral streptococcal strains, reflecting their bond stiffness, decreased after exposure to mouthrinses. Concurrently, the percentage detachment of adhering bacteria upon the passage of a liquid-air interface decreased after exposure to mouthrinses. A buffer control left both vibration amplitudes and detachment percentages unaffected. Exposure to either of the selected mouthrinses yielded more positively charged bacteria by particulate microelectrophoresis, suggesting antimicrobial adsorption to bacterial cell surface components. To rule out that exposure of adhering bacteria to the mouthrinses stimulated polysaccharide production with an impact on their detachment, Fourier transform infrared spectroscopy was carried out on bacteria adhering to an internal reflection element, prior to and after exposure to the mouthrinses. Infrared absorption band areas indicated no significant change in amount of polysaccharides after exposure of adhering bacteria to mouthrinses, but wave number shifts demonstrated stiffening of polysaccharides in the bond, as a result of antimicrobial adsorption to the bacterial cell surface and in line with changes in surface charge. Clinically, these findings suggest that accumulation of oral biofilm exposed to antimicrobials should be prevented (interdental cleaning aids, floss use), as removal becomes progressively more difficult upon multiple exposures.
Assuntos
Anti-Infecciosos/farmacologia , Aderência Bacteriana/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Boca/microbiologia , Streptococcus/efeitos dos fármacos , Cetilpiridínio/farmacologia , Clorexidina/análogos & derivados , Clorexidina/farmacologia , Fluoretos Tópicos/farmacologia , Antissépticos Bucais/farmacologia , Streptococcus mutans/efeitos dos fármacos , Streptococcus oralis/efeitos dos fármacosRESUMO
Biomaterial-implants are frequently used to restore function and form of human anatomy. However, the presence of implanted biomaterials dramatically elevates infection risk. Paradoxically, dental-implants placed in a bacteria-laden milieu experience moderate failure-rates, due to infection (0.0-1.1%), similar to the ones of joint-arthroplasties placed in a near-sterile environment (0.1-1.3%). Transcutaneous bone-fixation pins breach the immune-barrier of the epidermis, exposing underlying sterile-tissue to an unsterile external environment. In contrast to dental-implants, also placed in a highly unsterile environment, these pins give rise to relatively high infection-associated failure-rates of up to 23.0%. Herein, we attempt to identify causes as to why dental-implants so often succeed, where others fail. The major part of all implants considered are metal-made, with similar surface-finishes. Material choice was therefore discarded as underlying the paradox. Antimicrobial activity of saliva has also been suggested as a cause for the success of dental-implants, but was discarded because saliva is the implant-site-fluid from which viable bacteria adhere. Crevicular fluid was discarded as it is largely analogous to serum. Instead, we attribute the relative success of dental-implants to (1) ability of oral tissues to heal rapidly in the continuous presence of commensal bacteria and opportunistic pathogens, and (2) tolerance of the oral immune-system. Inability of local tissue to adhere, spread and grow in presence of bacteria and an intolerant immune-system are identified as the likely main causes explaining the susceptibility of other implants to infection-associated failure. In conclusion, it is the authors' belief that new anti-infection strategies for a wide range of biomaterial-implants may be derived from the relative success of dental-implants.
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Bactérias/crescimento & desenvolvimento , Implantes Dentários/normas , Próteses e Implantes/normas , Infecções Relacionadas à Prótese/microbiologia , Bactérias/classificação , Bactérias/efeitos dos fármacos , Materiais Biocompatíveis/química , Materiais Biocompatíveis/uso terapêutico , Implantação Dentária/métodos , Humanos , Imunidade nas Mucosas/imunologia , Modelos Biológicos , Mucosa Bucal/imunologia , Mucosa Bucal/microbiologia , Próteses e Implantes/classificação , Infecções Relacionadas à Prótese/imunologia , Infecções Relacionadas à Prótese/prevenção & controleRESUMO
A degradable, poly (lactic-co-glycolic acid) (PLGA), gentamicin-loaded prophylactic coating for hydroxyapatite (HA)-coated cementless hip prostheses is developed with similar antibacterial efficacy as offered by gentamicin-loaded cements for fixing traditional, cemented prostheses in bone. We describe the development pathway, from in vitro investigation of antibiotic release and antibacterial properties of this PLGA-gentamicin-HA-coating in different in vitro models to an evaluation of its efficacy in preventing implant-related infection in rabbits. Bone in-growth in the absence and presence of the coating was investigated in a canine model. The PLGA-gentamicin-HA-coating showed high-burst release, with antibacterial efficacy in agar-assays completely disappearing after 4 days, minimising risk of inducing antibiotic resistance. Gentamicin-sensitive and gentamicin-resistant staphylococci were killed by the antibiotic-loaded coating, in a simulated prosthesis-related interfacial gap. PLGA-gentamicin-HA-coatings prevented growth of bioluminescent staphylococci around a miniature-stem mounted in bacterially contaminated agar, as observed using bio-optical imaging. PLGA-gentamicin-HA-coated pins inserted in bacterially contaminated medullary canals in rabbits caused a statistically significant reduction in infection rates compared to HA-coated pins without gentamicin. Bone ingrowth to PLGA-gentamicin-HA-coated pins, in condylar defects of Beagle dogs was not impaired by the presence of the degradable, gentamicin-loaded coating. In conclusion, the PLGA-gentamicin-HA-coating constitutes an effective strategy for infection prophylaxis in cementless prostheses.
Assuntos
Materiais Revestidos Biocompatíveis/farmacologia , Durapatita/química , Gentamicinas/farmacologia , Prótese de Quadril , Infecções Relacionadas à Prótese/prevenção & controle , Infecções Estafilocócicas/prevenção & controle , Animais , Antibacterianos/química , Antibacterianos/farmacocinética , Antibacterianos/farmacologia , Cimentos Ósseos , Pinos Ortopédicos , Materiais Revestidos Biocompatíveis/química , Materiais Revestidos Biocompatíveis/farmacocinética , Cães , Farmacorresistência Bacteriana/efeitos dos fármacos , Feminino , Fêmur/cirurgia , Gentamicinas/química , Gentamicinas/farmacocinética , Ácido Láctico/química , Masculino , Microscopia Eletrônica de Varredura , Osseointegração/efeitos dos fármacos , Ácido Poliglicólico/química , Copolímero de Ácido Poliláctico e Ácido Poliglicólico , Infecções Relacionadas à Prótese/microbiologia , Coelhos , Infecções Estafilocócicas/microbiologia , Staphylococcus aureus/efeitos dos fármacos , Staphylococcus aureus/fisiologia , Staphylococcus epidermidis/efeitos dos fármacos , Staphylococcus epidermidis/fisiologia , Resultado do TratamentoRESUMO
Bacterial adhesion and subsequent biofilm formation on material surfaces represent a serious problem in society from both an economical and health perspective. Surface coating approaches to prevent bacterial adhesion and biofilm formation are of increased importance due to the increasing prevalence of antibiotic resistant bacterial strains. Effective antimicrobial surface coatings can be based on an anti-adhesive principle that prevents bacteria to adhere, or on bactericidal strategies, killing organisms either before or after contact is made with the surface. Many strategies, however, implement a multifunctional approach that incorporates both of these mechanisms. For anti-adhesive strategies, the use of polymer chains, or hydrogels is preferred, although recently a new class of super-hydrophobic surfaces has been described which demonstrate improved anti-adhesive activity. In addition, bacterial killing can be achieved using antimicrobial peptides, antibiotics, chitosan or enzymes directly bound, tethered through spacer-molecules or encased in biodegradable matrices, nanoparticles and quaternary ammonium compounds. Notwithstanding the ubiquitous nature of the problem of microbial colonization of material surfaces, this review focuses on the recent developments in antimicrobial surface coatings with respect to biomaterial implants and devices. In this biomedical arena, to rank the different coating strategies in order of increasing efficacy is impossible, since this depends on the clinical application aimed for and whether expectations are short- or long term. Considering that the era of antibiotics to control infectious biofilms will eventually come to an end, the future for biofilm control on biomaterial implants and devices is likely with surface-associated modifications that are non-antibiotic related.
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Antibacterianos/farmacologia , Bactérias/efeitos dos fármacos , Pesquisa Biomédica , Antibacterianos/química , Aderência Bacteriana/efeitos dos fármacos , Biofilmes/efeitos dos fármacos , Testes de Sensibilidade Microbiana , Propriedades de SuperfícieRESUMO
Biomaterial implants and devices increase the risk of microbial infections due to the biofilm mode of growth of infecting bacteria on implant materials, in which bacteria are protected against antibiotic treatment and the local immune system. Matrix-metalloproteinases (MMPs) and cell surface integrin receptors facilitate transmigration of inflammatory cells toward infected or inflamed tissue. This study investigates the relationship between MMP- and integrin-expression and the clearance of infecting Staphylococcus aureus around implanted biomaterials in a murine model.MMP- and integrin αvß3-expression were monitored in mice, with and without subcutaneously implanted biomaterial samples, in the absence and presence of bioluminescent S. aureus Xen36. Staphylococcal persistence was imaged longitudinally over time using bioluminescence imaging. The activatable MMPSense®680 and integrin-targeted IntegriSense®750 probes were injected on different days after implantation and their signal intensity and localisation monitored using fluorescence imaging. After sacrifice 7 or 16 days post-implantation, staphylococci from biomaterial samples and surrounding tissues were cultured on agar-plates and presence of host inflammatory cells was histologically evaluated.MMP- and integrin-expression were equally enhanced in presence of staphylococci or biomaterials up to 7 days post-implantation, but their localisation along the biomaterial samples differed. Bacterial clearance from tissue was higher in the absence of biomaterials. It is of clinical relevance that MMP- and integrin-expression were enhanced in presence of both staphylococci and biomaterials, although the immune system in the presence of biomaterials remained hampered in eradicating bacteria during the first 7 days post-implantation.
Assuntos
Biofilmes/crescimento & desenvolvimento , Implantes Experimentais/microbiologia , Integrina alfaVbeta3/metabolismo , Metaloproteinases da Matriz/metabolismo , Infecções Estafilocócicas/metabolismo , Animais , Integrina alfaVbeta3/genética , Metaloproteinases da Matriz/genética , Camundongos , Camundongos Endogâmicos BALB C , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Reação em Cadeia da Polimerase em Tempo Real , Infecções Estafilocócicas/patologiaRESUMO
OBJECTIVES: Oral biofilm is inevitably left behind, even after powered brushing. As a special feature, powered brushing removes biofilm in a noncontact mode. When the brushing distance becomes too large, biofilm is left behind. We hypothesize that biofilm left behind after brushing has different viscoelastic properties than before brushing, impacting antimicrobial penetration. MATERIALS AND METHODS: In vitro grown dual-species biofilms were subjected to 20 % mechanical deformation before and after powered brushing at 4-mm brushing distance. Biofilm thickness and stress relaxation were measured for unbrushed and brushed biofilms. Stress relaxation was analyzed with a three-element Maxwell model. Antimicrobial penetration from five mouthrinses was microscopically evaluated for unbrushed and brushed biofilms. RESULTS: Thicknesses of unbrushed and brushed biofilms were similar. Brushing decreased the prevalence of fast and increased the prevalence of slow relaxation elements, which was accompanied by deeper penetration of chlorhexidine and cetylpyridinium chloride. Penetration of antimicrobials from other mouthrinses was relatively low in unbrushed and brushed biofilms. CONCLUSIONS: This confirmation of our hypothesis points to an additional advantage of powered toothbrushing in a noncontact mode, changing the viscoelastic properties of biofilm in a direction that increases antimicrobial penetration of chlorhexidine and cetylpyridinium. CLINICAL RELEVANCE: The biofilm left behind after noncontact powered toothbrushing may have less recalcitrance toward penetration of chlorhexidine and cetylpyridinium chloride than prior to brushing.
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Anti-Infecciosos/administração & dosagem , Biofilmes , Escovação Dentária , Técnicas In VitroRESUMO
The oral microbiome consists of a planktonic microbiome residing in saliva and an adhering microbiome (the biofilm adhering to oral hard and soft tissues). Here we hypothesized that possible differences in microbial composition of the planktonic and adhering oral microbiome on teeth can be related to the forces by which different bacterial species are attracted to the tooth surface. The relative presence of 7 oral bacterial species in saliva and biofilm collected from 10 healthy human volunteers was determined twice in each volunteer by denaturing-gradient-gel electrophoresis. Analysis of both microbiomes showed complete separation of the planktonic from the adhering oral microbiome. Next, adhesion forces of corresponding bacterial strains with saliva-coated enamel surfaces were measured by atomic force microscopy. Species that were found predominantly in the adhering microbiome had significantly higher adhesion forces to saliva-coated enamel (-0.60 to -1.05 nN) than did species mostly present in the planktonic microbiome (-0.40 to -0.55 nN). It is concluded that differences in composition of the planktonic and the adhering oral microbiome are due to small differences in the forces by which strains adhere to saliva-coated enamel, providing an important step in understanding site- and material-specific differences in the composition of biofilms in the oral cavity.
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Aderência Bacteriana/fisiologia , Microbiota/fisiologia , Boca/microbiologia , Adulto , Animais , Biofilmes , Fenômenos Biomecânicos , Bovinos , Eletroforese em Gel de Gradiente Desnaturante , Esmalte Dentário/microbiologia , Película Dentária/microbiologia , Feminino , Humanos , Lactobacillus/isolamento & purificação , Lactobacillus/fisiologia , Lactobacillus acidophilus/isolamento & purificação , Lactobacillus acidophilus/fisiologia , Masculino , Microscopia de Força Atômica , Pessoa de Meia-Idade , Saliva/microbiologia , Streptococcus/isolamento & purificação , Streptococcus/fisiologia , Streptococcus mitis/isolamento & purificação , Streptococcus mitis/fisiologia , Streptococcus mutans/isolamento & purificação , Streptococcus mutans/fisiologia , Streptococcus oralis/isolamento & purificação , Streptococcus oralis/fisiologia , Streptococcus sanguis/isolamento & purificação , Streptococcus sanguis/fisiologia , Streptococcus sobrinus/isolamento & purificação , Streptococcus sobrinus/fisiologia , Dente/microbiologia , Adulto JovemRESUMO
Black pigmented conidia of Aspergillus niger give rise to micro-colonies when incubated in liquid shaken medium. These micro-colonies are heterogeneous with respect to gene expression and size. We here studied the biophysical properties of the conidia of a control strain and of strains in which the fwnA, olvA or brnA gene is inactivated. These strains form fawn-, olive-, and brown-coloured conidia, respectively. The ΔolvA strain produced larger conidia (3.8 µm) when compared to the other strains (3.2-3.3 µm). Moreover, the conidia of the ΔolvA strain were highly hydrophilic, whereas those of the other strains were hydrophobic. The zeta potential of the ΔolvA conidia in medium was also more negative when compared to the control strain. This was accompanied by the near absence of a rodlet layer of hydrophobins. Using the Complex Object Parametric Analyzer and Sorter it was shown that the ratio of individual hyphae and micro-colonies in liquid shaken cultures of the deletion strains was lower when compared to the control strain. The average size of the micro-colonies of the control strain was also smaller (628 µm) than that of the deletion strains (790-858 µm). The size distribution of the micro-colonies of the ΔfwnA strain was normally distributed, while that of the other strains could be explained by assuming a population of small and a population of large micro-colonies. In the last set of experiments it was shown that relative expression levels of gpdA, and AmyR and XlnR regulated genes correlate in individual hyphae at the periphery of micro-colonies. This indicates the existence of transcriptionally and translationally highly active and lowly active hyphae as was previously shown in macro-colonies. However, the existence of distinct populations of hyphae with high and low transcriptional and translational activity seems to be less robust when compared to macro-colonies grown on solid medium.
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The influence of fibronectin (Fn) coated surfaces patterned with poly(ethylene glycol) microgels having inter-gel spacings between 0.5 and 3.0 µm on the adhesion of Staphylococcus aureus strains with and without Fn-binding proteins and cellular adhesion/spreading was investigated. Quantitative force measurements between a S. aureus cell and a patterned surface showed that the adhesion force between the bacterium and the patterned surface increased substantially after Fn adsorption, regardless of the strain used, but decreased with decreasing inter-gel spacing. In flow-chamber experiments, the Fn-binding strain adhered at a higher rate after Fn adsorption than the strain lacking Fn-binding proteins. In both cases, the adhesion rates decreased with decreasing inter-gel spacing. Osteoblast-like cells could bind to patterned surfaces despite the microgels, and adsorbed Fn substantially amplified this effect. Even under highly non-adhesive conditions associated with closely spaced microgels, adsorbed Fn preserves a window of inter-gel spacing around 1 µm where the adhesion of staphylococcal cells is hindered while cells can still adhere and spread.
Assuntos
Adesinas Bacterianas/metabolismo , Aderência Bacteriana/efeitos dos fármacos , Adesão Celular/efeitos dos fármacos , Fibronectinas/farmacologia , Osteoblastos/fisiologia , Staphylococcus aureus/efeitos dos fármacos , Adsorção , Linhagem Celular Tumoral , Fibronectinas/química , Humanos , Osteoblastos/citologia , Staphylococcus aureus/metabolismo , Staphylococcus aureus/fisiologiaRESUMO
Many next-generation biomaterials will need the ability to not only promote healthy tissue integration but to simultaneously resist bacterial colonization and resulting biomaterials-associated infection. For this purpose, antimicrobial nanofibers of polycaprolactone (PCL) were fabricated by incorporating calcium peroxide. PCL nanofibers containing different ratios of calcium peroxide (1%, 5% and 10% (w/w)) with or without ascorbic acid were fabricated using an electrospinning technique. Antimicrobial evaluations confirmed the inhibitory properties of the nanofibers on the growth of E. coli and S. epidemidis because of a significant burst release of calcium peroxide from the nanofibers. Analysis of tissue cell response showed that despite an initial toxic effect over the first 24 h, after 4 days of culture, osteoblast viability and morphology were both healthy. These results demonstrate that oxygen-generating nanofibers can be designed and developed to provide a short-term peroxide-based antimicrobial response while still maintaining attractive tissue-integration properties.
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Anti-Infecciosos/química , Escherichia coli/efeitos dos fármacos , Nanofibras/química , Osteoblastos/citologia , Peróxidos/química , Anti-Infecciosos/farmacologia , Técnicas de Cultura de Células , Sobrevivência Celular/efeitos dos fármacos , Humanos , Microscopia Confocal , Peróxidos/farmacologiaRESUMO
Composite restorations degrade during wear, but it is unknown how wear affects the composite surface and influences composite-to-composite bonding in minimally invasive repair. Here, it is hypothesized that in vitro exposure of composites to oral biofilm yields clinically relevant degradation of composite surfaces, and its influence on composite-to-composite bonding is determined. Biofilms on composite surfaces in vitro increased their roughness and decreased filler particle exposure, except for a microhybrid composite, similar to effects of clinical wear in palatal appliances. Failure shear stresses after intermediate-adhesive-resin application were significantly lower after aging by in vitro exposure to biofilms, while silica-coating maintained the same failure stress levels as in non-aged composites. Failure modes were predominantly cohesive after silica-coating, while intermediate-adhesive-resin application yielded more adhesive failure. It is concluded that in vitro exposure to oral biofilm is a clinically relevant aging condition, and that silica-coating is to be preferred for the repair of aged composites.
Assuntos
Biofilmes , Resinas Compostas/química , Colagem Dentária , Materiais Dentários/química , Reparação de Restauração Dentária , Bactérias Gram-Negativas/fisiologia , Actinomyces/fisiologia , Adesividade , Candida albicans/fisiologia , Materiais Revestidos Biocompatíveis/química , Desgaste de Restauração Dentária , Análise do Estresse Dentário/instrumentação , Fusobacterium nucleatum/fisiologia , Humanos , Teste de Materiais , Polimerização , Silanos/química , Dióxido de Silício/química , Streptococcus oralis/fisiologia , Streptococcus sobrinus/fisiologia , Estresse Mecânico , Propriedades de Superfície , Fatores de Tempo , Veillonella/fisiologiaRESUMO
The effects of peracetic acid-based (PAA) disinfectant with, and without, additional drying on Candida albicans, Candida parapsilosis, Pseudomonas aeruginosa and Stenotrophomonas maltophilia, isolated from contaminated flexible endoscopes, in single- and dual-species biofilms were studied. Biofilms were prepared in sterile tissue culture polystyrene 96-well microtitre plates and were quantified using the tetrazolium salt (MTT) reduction assay and by counting colony-forming yeasts and bacteria from 10-fold serial biofilm dilutions on agar plates. An in vitro biofilm model was applied to mimic the biofilm formation inside the endoscope channels and to imitate the disinfection and drying procedures used for reprocessing of flexible endoscopes. The PAA-based disinfectant was effective against bacteria and yeasts in the planktonic and biofilm states directly after treatment, but allowed regrowth of all biofilms if the drying procedure was skipped. No biofilm regrowth occurred in wells after a drying procedure in all single- and dual-species biofilms. Routine cleaning procedures do not remove biofilm reliably from endoscope channels if the accurate drying procedure is not applied. This may explain the failure of decontamination during endoscope reprocessing.
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Biofilmes/efeitos dos fármacos , Candida/efeitos dos fármacos , Desinfetantes/farmacologia , Endoscópios/microbiologia , Ácido Peracético/farmacologia , Pseudomonas aeruginosa/efeitos dos fármacos , Stenotrophomonas maltophilia/efeitos dos fármacos , Candida/fisiologia , Desinfecção/métodos , Humanos , Viabilidade Microbiana/efeitos dos fármacos , Modelos Biológicos , Pseudomonas aeruginosa/fisiologia , Stenotrophomonas maltophilia/fisiologia , Sais de Tetrazólio/metabolismo , Tiazóis/metabolismoRESUMO
The aim of this study was to determine the contribution of plaque and saliva towards the prolonged activity, also called substantivity, of three antimicrobial mouthrinses (Listerine®, Meridol®, Crest Pro Health®), used in combination with a toothpaste (Prodent Coolmint®). Volunteers brushed for 4 weeks with a toothpaste without antimicrobial claims, while during the last 2 weeks half of the volunteers used an antimicrobial mouthrinse in addition to brushing. At the end of the experimental period, plaque and saliva samples were collected 6 h after oral hygiene, and bacterial concentrations and viabilities were determined. The contribution of plaque and saliva towards substantivity was assessed by combining plaque obtained after mechanical cleaning only with plaque and saliva obtained after additional use of an antimicrobial rinse. Subsequently, resulting viabilities of the combined plaques were determined. The viabilities of plaque samples after additional rinsing with mouthrinses were lower than of plaque obtained after mechanical cleaning only, regardless of the rinse involved. Moreover, plaque collected 6 h after rinsing with antimicrobial mouthrinses contained a surplus of antimicrobial activity. Only Listerine showed decreased viability in saliva, but none of the mouthrinses showed any residual antimicrobial activity in saliva. The findings indicate that plaque left behind after mechanical cleaning contributes to the prolonged substantivity of antimicrobial mouthrinses.
Assuntos
Anti-Infecciosos Locais/metabolismo , Placa Dentária/metabolismo , Antissépticos Bucais/metabolismo , Saliva/metabolismo , Adulto , Aminas/metabolismo , Aminas/uso terapêutico , Anti-Infecciosos Locais/uso terapêutico , Bactérias/efeitos dos fármacos , Carga Bacteriana , Cetilpiridínio/metabolismo , Cetilpiridínio/uso terapêutico , Corantes , Placa Dentária/microbiologia , Combinação de Medicamentos , Feminino , Humanos , Masculino , Viabilidade Microbiana/efeitos dos fármacos , Antissépticos Bucais/uso terapêutico , Salicilatos/metabolismo , Salicilatos/uso terapêutico , Saliva/microbiologia , Terpenos/metabolismo , Terpenos/uso terapêutico , Fatores de Tempo , Fluoretos de Estanho/metabolismo , Fluoretos de Estanho/uso terapêutico , Escovação Dentária , Cremes Dentais/uso terapêutico , Adulto JovemRESUMO
Biomaterials for the restoration of oral function are prone to biofilm formation, affecting oral health. Oral bacteria adhere to hydrophobic and hydrophilic surfaces, but due to fluctuating shear, little biofilm accumulates on hydrophobic surfaces in vivo. More biofilm accumulates on rough than on smooth surfaces. Oral biofilms mostly consist of multiple bacterial strains, but Candida species are found on acrylic dentures. Biofilms on gold and amalgam in vivo are thick and fully covering, but barely viable. Biofilms on ceramics are thin and highly viable. Biofilms on composites and glass-ionomer cements cause surface deterioration, which enhances biofilm formation again. Residual monomer release from composites influences biofilm growth in vitro, but effects in vivo are less pronounced, probably due to the large volume of saliva into which compounds are released and its continuous refreshment. Similarly, conflicting results have been reported on effects of fluoride release from glass-ionomer cements. Finally, biomaterial-associated infection of implants and devices elsewhere in the body is compared with oral biofilm formation. Biomaterial modifications to discourage biofilm formation on implants and devices are critically discussed for possible applications in dentistry. It is concluded that, for dental applications, antimicrobial coatings killing bacteria upon contact are more promising than antimicrobial-releasing coatings.
Assuntos
Materiais Biocompatíveis/química , Biofilmes/crescimento & desenvolvimento , Implantes Dentários/microbiologia , Materiais Dentários/química , Restauração Dentária Permanente , Anti-Infecciosos/química , Aderência Bacteriana/fisiologia , Adesão Celular/fisiologia , Materiais Revestidos Biocompatíveis/química , Humanos , Interações Hidrofóbicas e Hidrofílicas , Propriedades de SuperfícieRESUMO
Non-contact removal of oral biofilms offers advantages beyond the reach of bristles, but it is unknown how energy transfer for removal from brush-to-biofilm occurs. In the present study we evaluated non-contact, oral biofilm removal by oscillating-rotating and sonic toothbrushes, and their acoustic output up to 6 mm distance. Whereas some brushes removed biofilm when used at a distance of up to 6 mm, others lost efficacy at a distance of 2-4 mm from the biofilm. Loss of efficacy was accompanied with high standard deviations and volumetric biofilm expansion. Both sonic and oscillating-rotating brushes caused fluid flows and the inclusion of air-bubbles, while non-contact acoustic energy-transfer was demonstrated to decay with distance for both types of brushes. We put forward the following mechanism for non-contact removal: (i) brush energy is absorbed by biofilm, resulting in the visco-elastic expansion of the biofilm; (ii) if the energy absorbed is sufficient and deformation is beyond the yield point, biofilm removal occurs; and (iii) if deformation is in the plastic range but below the yield point (i.e. at the limiting distance for non-contact removal), biofilm is expanded but not removed.
Assuntos
Actinomyces/fisiologia , Biofilmes , Streptococcus oralis/fisiologia , Escovação Dentária/métodos , Absorção , Acústica , Aderência Bacteriana , Fenômenos Biomecânicos , Película Dentária/microbiologia , Módulo de Elasticidade , Transferência de Energia , Desenho de Equipamento , Humanos , Microscopia Confocal , Reologia , Rotação , Saliva/microbiologia , Escovação Dentária/instrumentação , ViscosidadeRESUMO
Biomaterials-associated-infections (BAI) are serious complications in modern medicine. Although non-adhesive coatings, like polymer-brush coatings, have been shown to prevent bacterial adhesion, they do not support cell growth. Bi-functional coatings are supposed to prevent biofilm formation while supporting tissue integration. Here, bacterial and cellular responses to poly(ethylene glycol) (PEG) brush-coatings on titanium oxide presenting the integrin-active peptide RGD (arginine-glycine-aspartic acid) (bioactive "PEG-RGD") were compared to mono-functional PEG brush-coatings (biopassive "PEG") and bare titanium oxide (TiO2) surfaces under flow. Staphylococcus epidermidis ATCC 35983 was deposited on the surfaces under a shear rate of 11 s-1 for 2 h followed by seeding of U2OS osteoblasts. Subsequently, both S. epidermidis and U2OS cells were grown simultaneously on the surfaces for 48 h under low shear (0.14 s-1). After 2 h, staphylococcal adhesion was reduced to 3.6-/+1.8 x 103 and 6.0-/+3.9 x 103 cm-2 on PEG and PEG-RGD coatings respectively, compared to 1.3-/+0.4 x 105 cm-2 for the TiO2 surface. When allowed to grow for 48 h, biofilms formed on all surfaces. However, biofilms detached from the PEG and PEG-RGD coatings when exposed to an elevated shear (5.6 s-1) U2OS cells neither adhered nor spread on PEG brush-coatings, regardless of the presence of biofilm. In contrast, in the presence of biofilm, U2OS cells adhered and spread on PEG-RGD coatings with a significantly higher surface coverage than on bare TiO2. The detachment of biofilm and the high cell surface coverage revealed the potential significance of PEG-RGD coatings in the context of the "race for the surface" between bacteria and mammalian cells.