RESUMO
Mycoparasites in Basidiomycota comprise a diverse group of fungi, both morphologically and phylogenetically. They interact with their hosts through either fusion-interaction or colacosome-interaction. Colacosomes are subcellular structures formed by the mycoparasite at the host-parasite interface, which penetrate the parasite and host cell walls. Previously, these structures were detected in 19 fungal species, usually by means of transmission electron microscopy. Most colacosome-forming species have been assigned to Microbotryomycetes (Pucciniomycotina, Basidiomycota), a highly diverse class, comprising saprobic yeasts, mycoparasites, and phytoparasites. In general, these myco- and phytoparasites are dimorphic organisms, with a parasitic filamentous morph and saprobic yeast morph. We investigated colacosome-forming mycoparasites based on fungarium material, freshly collected specimens, and cultures of yeast morphs. We characterised the micromorphology of filamentous morphs, the physiological characteristics of yeast morphs, and inferred phylogenetic relationships based on DNA sequence data from seven loci. We outline and employ an epifluorescence-based microscopic method to assess the presence and organisation of colacosomes. We describe five new species in the genus Colacogloea, the novel dimorphic mycoparasite Mycogloiocolax gerardii, and provide the first report of a sexual, mycoparasitic morph in Colacogloea philyla and in the genus Slooffia. We detected colacosomes in eight fungal species, which brings the total number of known colacosome-forming fungi to 27. Finally, we revealed three distinct types of colacosome organisation in Microbotryomycetes. Taxonomic novelties and typifications: New family: Mycogloiocolacaeae Schoutteten & Yurkov; New genus: Mycogloiocolax Schoutteten & Rödel; New species: Colacogloea bettinae Schoutteten & Begerow, C. biconidiata Schoutteten, C. fennica Schoutteten & Miettinen, C. microspora Schoutteten, C. universitatis-gandavensis Schoutteten & Verbeken, Mycogloiocolax gerardii Schoutteten & Rödel; New combinations: Slooffia micra (Bourdot & Galzin) Schoutteten, Fellozyma cerberi (A.M. Yurkov et al.) Schoutteten & Yurkov, Fellozyma telluris (A.M. Yurkov et al.) Schoutteten & Yurkov; Epitypifications (basionyms): Achroomyces insignis Hauerslev, Platygloea micra Bourdot & Galzin, Platygloea peniophorae Bourdot & Galzin; Lectotypification (basionym): Platygloea peniophorae Bourdot & Galzin Citation: Schoutteten N, Yurkov A, Leroux O, Haelewaters D, Van Der Straeten D, Miettinen O, Boekhout T, Begerow D, Verbeken A (2023). Diversity of colacosome-interacting mycoparasites expands the understanding of the evolution and ecology of Microbotryomycetes. Studies in Mycology 106: 41-94. doi: 10.3114/sim.2022.106.02.
RESUMO
BACKGROUND: Grafting is a technique widely used in horticulture that also has been applied in agriculture. In plant physiology, grafting facilitates the elucidation of mechanisms underlying growth and developmental processes, through the construction of chimeric plants with organs of different genotypes. Despite its small size, the model species Arabidopsis thaliana is very amenable for grafting, which can be useful to investigate transport of nutrients, amino acids or secondary metabolites between different tissues, or to investigate developmental processes depending on root-to-shoot communication, such as shoot branching, root and shoot plasticity upon shade avoidance, or disease resistance. Nevertheless, grafting protocols are usually technically challenging and training is required to achieve a reasonable success rate. Additionally, specialized tools and equipment are often needed, such as chips to accommodate the grafted plantlets or collars to maintain the contact between root and shoot. RESULTS: In this methodology paper, we provide a fast, easy, accessible, and specialized equipment-free protocol that enables high success ratios. Critical steps and notes are detailed, easing the implementation of the procedure for non-trained researchers. An example of the protocol application by three independent non-trained researchers shows that this method allows to achieve a 90-100% of grafting efficiency after 6 days post-grafting recovery. In addition, the grafting of Col-0 with the acs8x mutant, depleted in 1-aminocyclopropane-1-carboxylic acid (ACC), the biosynthetic precursor of the phytohormone ethylene, provides an example of the application of this optimized protocol, showing the suitability of the process to study long-distance transport processes. CONCLUSIONS: We present an optimized protocol for hypocotyl grafting of 4-day-old Arabidopsis thaliana seedlings. The combination of conditions yields a grafting success of 90-100% and provides an easy and accessible methodology, reducing the time frame, and without the necessity of acquiring specialized equipment. The presented protocol is simple, fast and highly efficient, easing the inclusion of hypocotyl grafting assays in any research project. In addition, the description of the protocol is detailed to a level ensuring that even non-trained researchers, are sufficiently prepared to adopt the grafting methodology.
RESUMO
Tetrahydrofolate and its derivatives, commonly known as folates, are essential for almost all living organisms. Besides acting as one-carbon donors and acceptors in reactions producing various important biomolecules such as nucleic and amino acids, as well as pantothenate, they also supply one-carbon units for methylation reactions. Plants along with bacteria, yeast and fungi synthesize folates de novo and therefore constitute a very important dietary source of folates for animals. All the major steps of folate biosynthesis and metabolism have been identified but only few have been genetically characterized in a handful of model plant species. The possible differences in the folate pathway between various plant and algal species have never been explored. In this study we present a comprehensive comparative study of folate biosynthesis and metabolism of all major land plant lineages as well as green and red algae. The study identifies new features of plant folate metabolism that might open new directions to folate research in plants.
Assuntos
Clorófitas/metabolismo , Embriófitas/metabolismo , Evolução Molecular , Ácido Fólico/metabolismo , Rodófitas/metabolismo , Ácido Fólico/biossínteseRESUMO
Antifolates can impair the synthesis and/or function of folates in living organisms. Mechanisms of resistance or tolerance to antifolates have been mainly described in plants using the drug methotrexate. In this work, the antifolate trimethoprim (TMP) was used with the aim of revealing a novel mechanism of resistance. EMS mutagenised seeds from Arabidopsis were screened to isolate individuals insensitive to TMP. Genetic analysis revealed a homozygous recessive mutation that segregates with the phenotype of tolerance to 50 µm TMP. Mapping analysis localised the mutation at the end of the short arm of chromosome 3. Preliminary characterisation demonstrated up-regulation of several genes from the folate biosynthetic pathway in the TMP insensitive mutant, and a slight increase in total folate content in the mutant as compared with the Col-0 control. Moreover, sequence analysis of the DHFR (dihydrofolate reductase) genes, which encode a known target for resistance to antifolates, did not reveal any changes. This study is the first report of a stable mutant insensitive (afi1) to the antifolate trimethoprim in plants, and suggests the existence of a novel mechanism of resistance to antifolates.
Assuntos
Arabidopsis/genética , Antagonistas do Ácido Fólico/farmacologia , Ácido Fólico/metabolismo , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Trimetoprima/farmacologia , Arabidopsis/efeitos dos fármacos , Arabidopsis/metabolismo , Proteínas de Arabidopsis/genética , Mapeamento Cromossômico , Cromossomos de Plantas/genética , Ácido Fólico/análise , Ácido Fólico/química , Antagonistas do Ácido Fólico/química , Marcadores Genéticos , Metotrexato/química , Metotrexato/farmacologia , Mutação , Fenótipo , Plântula/efeitos dos fármacos , Plântula/genética , Plântula/metabolismo , Análise de Sequência de DNA , Tetra-Hidrofolato Desidrogenase/genética , Trimetoprima/química , Regulação para Cima/efeitos dos fármacosRESUMO
Neural-tube defects (NTDs) are considered to be the most common congenital malformations. As Shanxi Province, a poor region in the North of China, has one of the highest reported prevalence rates of NTDs in the world, folate fortification of rice is an excellent alternative to low intake of folate acid pills in this region. This paper investigates the relations between socio-demographic indicators, consumer characteristics (knowledge, consumer perceptions on benefits, risks, safety and price), willingness-to-accept and willingness-to-pay genetically modified (GM) rice. The consumer survey compromises 944 face-to-face interviews with rice consumers in Shanxi Province, China. Multivariate analyses consist of multinomial logistic regression and multiple regression. The results indicate that consumers generally are willing-to-accept GM rice, with an acceptance rate of 62.2%. Acceptance is influenced by objective knowledge and consumers' perceptions on benefits and risks. Willingness-to-pay GM rice is influenced by objective knowledge, risk perception and acceptance. Communication towards the use of GM rice should target mainly improving knowledge and consumers' perceptions on high-risk groups within Shanxi Province, in particular low educated women.
Assuntos
Atitude Frente a Saúde , Comportamento do Consumidor/estatística & dados numéricos , Ácido Fólico/administração & dosagem , Alimentos Fortificados/estatística & dados numéricos , Alimentos Geneticamente Modificados/estatística & dados numéricos , Oryza , Adulto , Distribuição por Idade , China , Comportamento do Consumidor/economia , Feminino , Alimentos Fortificados/economia , Alimentos Geneticamente Modificados/economia , Conhecimentos, Atitudes e Prática em Saúde , Promoção da Saúde/economia , Promoção da Saúde/métodos , Humanos , Intenção , Entrevistas como Assunto , Masculino , Pessoa de Meia-Idade , Defeitos do Tubo Neural/prevenção & controle , Percepção/fisiologia , Fatores Socioeconômicos , Inquéritos e Questionários , Adulto JovemRESUMO
In the past decade, the understanding of folate bioavailability, metabolism and related health issues has increased, but several problems remain, including the difficulty of delivering the available knowledge to the populations at risk. Owing to the low compliance of taking folic acid supplements, for example, among women of child-bearing age who could lower the risk of having a baby with a neural tube defect, food-based strategies aimed at increasing the intake of folate and other B-group vitamins should be a priority for future research. These should include the development of a combined strategy of supplemental folate (possibly with vitamin B(12)), biofortification using engineered plant-derived foods and micro-organisms and food fortification for increasing folate intakes in the general population. Currently, the most effective population-based strategy to reduce NTDs remains folic acid fortification. However, the possible adverse effect of high intakes of folic acid on neurologic functioning among elderly persons with vitamin B(12) deficiency needs urgent investigation. The results of ongoing randomized controlled studies aimed at reducing the prevalence of hyperhomocysteinemia and related morbidity must be available before food-based total population approaches for treatment of hyperhomocysteinemia can be recommended. Further research is required on quantitative assessment of folate intake and bioavailability, along with a more thorough understanding of physiological, biochemical and genetic processes involved in folate absorption and metabolism.
Assuntos
Ácido Fólico/administração & dosagem , Ácido Fólico/farmacocinética , Hiper-Homocisteinemia/prevenção & controle , Defeitos do Tubo Neural/prevenção & controle , Complexo Vitamínico B/administração & dosagem , Complexo Vitamínico B/farmacocinética , Disponibilidade Biológica , Ácido Fólico/metabolismo , Tecnologia de Alimentos , Alimentos Fortificados , Humanos , Absorção Intestinal , Vitamina B 12/administração & dosagem , Complexo Vitamínico B/metabolismoRESUMO
Treatment of the Arabidopsis thaliana root with the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (ACC) immediately imposes a reduced maximal cell length beyond which further elongation is blocked. Here, we investigated possible apoplastic reactions involved in the inhibition of cell elongation. Five-day-old Arabidopsis seedlings were transferred to a growth medium supplemented with ACC and the effect on root cell length was recorded after 3 h of treatment. Altered characteristics in the apoplast of the nonelongating cells in the ACC-treated root, such as 'reactive oxygen species' (ROS) production and callose deposition, were detected using specific fluorochromes. The presence of functional hydroxyproline-rich glycoproteins (HRGPs) and the crosslinking of these cell-wall proteins are essential in limiting cell elongation. The ROS that drive the oxidative crosslinking of HRGPs, accumulate in the apoplast of cells in the zone where cell elongation stops. In the same cells, callose is deposited in the cell wall. The final cell length in the Arabidopsis root treated for a short period with ACC is determined in the zone of fast elongation. Both HRGPs crosslinking by ROS and callose deposition in the cell wall of this zone are suggested as causes for the reduced cell elongation.
Assuntos
Aminoácidos Cíclicos/farmacologia , Arabidopsis/crescimento & desenvolvimento , Tamanho Celular/efeitos dos fármacos , Raízes de Plantas/anatomia & histologia , Arabidopsis/citologia , Proteínas de Arabidopsis/efeitos dos fármacos , Proteínas de Arabidopsis/metabolismo , Parede Celular/metabolismo , Glicina/análogos & derivados , Glicina/farmacologia , Hidroxiprolina/metabolismo , Proteínas de Plantas/metabolismo , Raízes de Plantas/efeitos dos fármacos , Espécies Reativas de Oxigênio/metabolismoRESUMO
The management of limited fresh water resources is a major challenge facing society in the 21st century. The agricultural sector accounts for more than two-thirds of human water withdrawal and is therefore a prime area to implement a more rational water use. Environmental stresses are a major factor limiting stable food production. Given the growing shortage of available water for crops this will be an emerging factor in international agricultural economy. The most environmentally friendly and durable solution to the problem of water shortage is to complement more efficient irrigation approaches with crops with optimal water use efficiency, achieved either through genetic engineering or conventional breeding, combined with high yields.
Assuntos
Conservação dos Recursos Naturais/tendências , Produtos Agrícolas/genética , Epiderme Vegetal/genética , Plantas Geneticamente Modificadas/genética , Água/metabolismo , Produtos Agrícolas/metabolismo , Epiderme Vegetal/metabolismo , Folhas de Planta/genética , Folhas de Planta/metabolismo , Plantas Geneticamente Modificadas/metabolismo , Abastecimento de ÁguaRESUMO
The resistance to rice blast disease in the Vietnamese traditional rice cultivar 'Chiembac' was studied. The blast resistance spectrum in 'Chiembac' and 15 rice differentials carrying different known resistance genes was identified using 25 Pyricularia grisea isolates derived from 15 AFLP lineages from the North, Center and South of Vietnam. None of the differential lines carrying a single resistance gene could effectively control all tested Vietnamese blast isolates. 'Chiembac' showed a different resistance pattern compared to that of the differential lines. A cross between 'Chiembac' and 'CR203', an improved rice cultivar, was made and the F2 population was used for characterization and mapping of the resistance genes in 'Chiembac'. Genetic analysis showed that the resistance against two representative isolates from two predominant lineages, VT7 and 12, in 'Chiembac' was controlled by the single dominant genes Pi-VT7 and Pi-I2. The resistance gene Pi-VT7 was closely linked to Pi-I2 and was mapped to chromosome 12 using the framework mapping population 'IR64' x 'Azucena' of 124 double haploid progenies. The resistance to the Vietnamese blast isolate VT7 in 'IR64' was also studied. The latter was controlled by one locus with major effect located on chromosome 12 and mapped closely to the AFLP marker NIN080, which was also tightly linked to the resistance gene Pi-VT7 in 'Chiembac'. Thus, the resistance locus Pi-VT7 and the resistance locus in 'IR64' probably belong to a cluster of resistance genes.
Assuntos
Oryza/genética , Oryza/microbiologia , Doenças das Plantas/genética , Doenças das Plantas/microbiologia , Pyricularia grisea/fisiologia , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , Cruzamento , VietnãRESUMO
Historically, early stress-induced changes in plants have been mainly detected after destructive sampling followed by biochemical and molecular determinations. Imaging techniques that allow immediate detection of stress-situations, before visual symptoms appear and adverse effects become established, are emerging as promising tools for crop yield management. Such monitoring approaches can also be applied to screen plant populations for mutants with increased stress tolerance. At the laboratory scale, different imaging methods can be tested and one or a combination best suited for crop surveillance chosen. The system of choice can be applied under controlled laboratory conditions to guide selective sampling for the molecular characterisation of rapid stress-induced changes. Such an approach permits to isolate presymptomatically induced genes, or to obtain a panoramic view of early gene expression using gene-arrays when plants undergo physiological changes undetected by the human eye. Using this knowledge, plants can be engineered to be more stress resistant, and tested for field performance by the same methodologies. In ongoing efforts of genome characterisation, genes of unknown function are revealed at an ever-accelerating pace. By monitoring changes in phenotypic characteristics of transgenic plants expressing those genes, imaging techniques could help to identify their function.
Assuntos
Fenômenos Fisiológicos Vegetais , Medições Luminescentes , Imageamento por Ressonância Magnética , Microscopia de Fluorescência , TemperaturaRESUMO
Arabidopsis thaliana plants were sprayed with the superoxide-generating herbicide paraquat. The headspace of sprayed plants was characterized by a number of compounds, which were absent in the headspace of untreated plants. They were identified as isothiocyanates (ITCs) with 4-methylthiobutyl isothiocyanate as main compound. After identification, a GC-system, based on PDMS sorption, was used to continuously monitor the ITC emissions. The specificity of isothiocyanate emission was also determined by subjecting the Arabidopsis thaliana plants to in vitro mechanical wounding. Again, 4-methylthiobutyl isothiocyanate was the main component, but the emission profile was completely different since the compound was emitted immediately, i.e., during wounding itself.
Assuntos
Arabidopsis/química , Herbicidas , Isotiocianatos/análise , Paraquat , Cromatografia Gasosa-Espectrometria de Massas , VolatilizaçãoRESUMO
Survival of rice (Oryza sativa) upon an extreme rise of the water level depends on rapid stem elongation, which is mediated by ethylene. A genomic clone (OS-ACS5) encoding 1-aminocyclopropane-1-carboxylic acid (ACC) synthase, which catalyzes a regulatory step in ethylene biosynthesis, has been isolated from cv IR36, a lowland rice variety. Expression was induced upon short- and long-term submergence in cv IR36 and in cv Plai Ngam, a Thai deepwater rice variety. Under hypoxic conditions, abscisic acid and gibberellin had a reciprocal opposite effect on the activity of OS-ACS5. Gibberellin up-regulated and abscisic acid down-regulated OS-ACS5 mRNA accumulation. Growth experiments indicated that lowland rice responded to submergence with a burst of growth early on, but lacked the ability to sustain elongation growth. Sustained growth, characteristic for deepwater rice, was correlated with a prolonged induction of OS-ACS5. In addition, a more pronounced capacity to convert ACC to ethylene, a limited ACC conjugation, and a high level of endogenous gibberellin(20) were characteristic for the deepwater variety. An elevated level of OS-ACS5 messenger was found in cv IR36 plants treated with exogenous ACC. This observation was concomitant with an increase in the capacity of converting ACC to ethylene and in elongation growth, and resulted in prolonged survival. In conclusion, OS-ACS5 is involved in the rapid elongation growth of deepwater rice by contributing to the initial and long-term increase in ethylene levels. Our data also suggest that ACC limits survival of submerged lowland rice seedlings.
Assuntos
Oryza/crescimento & desenvolvimento , Oryza/genética , Agricultura/métodos , Sequência de Bases , Clorofila/metabolismo , Cicloeximida/farmacologia , Dados de Sequência Molecular , Oryza/efeitos dos fármacos , Reação em Cadeia da Polimerase , Regiões Promotoras Genéticas , RNA Mensageiro/química , RNA Mensageiro/genética , Alinhamento de Sequência , Homologia de Sequência do Ácido Nucleico , ÁguaRESUMO
Salicylic acid (SA), produced by plants as a signal in defense against pathogens, induces metabolic heating mediated by alternative respiration in flowers of thermogenic plants, and, when exogenously applied, increases leaf temperature in nonthermogenic plants. We have postulated that the latter phenomenon would be detectable when SA is synthesized locally in plant leaves. Here, resistance to tobacco mosaic virus (TMV) was monitored thermographically before any disease symptoms became visible on tobacco leaves. Spots of elevated temperature that were confined to the place of infection increased in intensity from 8 h before the onset of visible cell death, and remained detectable as a halo around the ongoing necrosis. Salicylic acid accumulates during the prenecrotic phase in TMV-infected tobacco and is known to induce stomatal closure in certain species. We show that the time course of SA accumulation correlates with the evolution of both localized thermal effect and stomatal closure. Since the contribution of leaf respiration is marginal, we concluded that the thermal effect results predominantly from localized, SA-induced stomatal closure. The presymptomatic temperature increase could be of general significance in incompatible plant-pathogen interactions.
Assuntos
Nicotiana/virologia , Plantas Tóxicas , Vírus do Mosaico do Tabaco/fisiologia , Fenômenos Fisiológicos Vegetais , Ácido Salicílico/metabolismo , Termografia , Nicotiana/metabolismoRESUMO
A simple and rapid PCR-based method for screening transformed Arabidopsis plants has been developed. Based on the quantity of chlorophyll present in a protoplast suspension, the optimal amount of template is calculated and a fragment of the transgene is amplified.
Assuntos
Arabidopsis/genética , Reação em Cadeia da Polimerase/métodos , Primers do DNA , Plantas Geneticamente Modificadas/genética , Reprodutibilidade dos TestesRESUMO
GT-2 is a plant transcriptional activator that contains two separate, but similar, trihelix DNA-binding domains. GT-1 is similar to GT-2, although it contains only one of such domains. cDNAs that encode GT-2 were isolated from rice (OS-GT2) and Arabidopsis (AT-GT2). Evidence is presented for the existence of an Arabidopsis gene family that is structurally related to AT-GT2. Two members of this GT2-like family, AT-GTL1 and AT-GTL2, have been isolated and characterized. Their sequences suggest that they evolved by a recent gene duplication event. Both AT-GT2 and AT-GTL genes contain an intron in the amino-terminal trihelix motif, indicating that this DNA-binding domain resulted from exon shuffling. RNA gel blot analysis using AT-GTL1 as a probe revealed four transcripts in the aerial part of the plant. All mRNA levels were significantly higher in siliques, suggesting that this gene family may function in fruit and/or seed development. To date, DNA-binding proteins characterized by the trihelix motif have been described only in plants, and may therefore be involved in plant-specific processes. Our results show that in Arabidopsis thaliana, the trihelix motif is not restricted to the GT-1 and GT-2 DNA-binding proteins.
Assuntos
Proteínas de Arabidopsis , Arabidopsis/genética , Proteínas de Ligação a DNA/genética , Genes de Plantas , Família Multigênica , Proteínas de Plantas/genética , Sequência de Aminoácidos , Sítios de Ligação , Evolução Molecular , Biblioteca Genômica , Modelos Genéticos , Dados de Sequência Molecular , Polimorfismo de Fragmento de Restrição , RNA Mensageiro/análise , RNA de Plantas/análise , Mapeamento por Restrição , Análise de Sequência de DNA , Homologia de Sequência de AminoácidosRESUMO
A novel cDNA clone osr40cl, encoding a abscisic acid (ABA)-responsive 40-kDa protein previously associated with salt tolerance (Moons et al. '1995' Plant Physiol 107: 177-186), was isolated from roots of rice seedlings (Oryza sativa L.). Exogenously applied ABA and salt shock induced a marked increase of the osr40cl transcript level in roots of seedlings whereas constant osr40cl mRNA levels were found in the shoot. The root-specific salinity-induced osr40cl mRNA accumulation was rapid and gradually declined upon prolonged salt shock. Plant growth regulators, signalling the wounding and the pathogen response, did not enhance osr40cl expression, indicating a salt- and osmotic-stress-specific response. The encoded OSR40cl protein was found to be hydrophilic, rich in histidine residues (6%) constituting putative metal-binding domains, and to consist of a duplicated domain of 151 amino acids (75% identical), that can form amphiphilic alpha-helical structures. The gene osr40cl belongs to a multigene family. Two osr40 genes were isolated, osr40g2 and osr40g3, tandemly arranged in an 8-kb region of the rice genome. Antisera raised against a conserved OSR40 peptide recognized different OSR40 proteins that accumulated in roots upon exposure to salt stress. The OSR40 protein family included 29-KDa proteins and two 40-kDa proteins, the latter most probably corresponding to OSR40cl and OSR40g2 with duplicated domain structures. The osr40g3 transcript encoded a single copy of the OSR40 domain and exhibited a shoot-specific expression. Results indicate that OSR40cl plays a role in the adaptive response of roots to an hyper-osmotic environment and belongs to a novel plant protein family that most probably has structural functions.
Assuntos
Ácido Abscísico/farmacologia , DNA de Plantas/metabolismo , Genes de Plantas , Família Multigênica , Oryza/genética , Oryza/metabolismo , Proteínas de Plantas/biossíntese , Transcrição Gênica , Sequência de Aminoácidos , Sequência de Bases , Clonagem Molecular , DNA Complementar , DNA de Plantas/química , Dados de Sequência Molecular , Concentração Osmolar , Proteínas de Plantas/química , Proteínas de Plantas/genética , Raízes de Plantas , Estrutura Secundária de Proteína , RNA Mensageiro/biossíntese , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Alinhamento de Sequência , Homologia de Sequência de Aminoácidos , Cloreto de Sódio/farmacologia , Transcrição Gênica/efeitos dos fármacosRESUMO
Young chicory plants (Cichorium intybus L. var. Witloof) show a tenfold higher nitrate reductase NR activity in roots compared to leaves. Northern analysis revealed, besides the nitrate inducibility of the nitrate reductase gene (nia), a higher level of expression in the roots. By modifying the external nitrate concentration the NR activity in the leaves remained negligible whereas a maximal activity was observed in the roots when grown in the presence of 5 mM nitrate. Surprisingly, variation of the external nitrate concentration induced changes in the spatial regulation of nia within the root. In-situ hybridization mainly localized nia mRNA in the cortical cells of roots grown at low nitrate concentrations (0.2 nM). At high nitrate concentrations (5 mM), nia mRNA was more abundant in the vascular tissues. The root apex revealed a strong signal under both conditions. The isolation and characterization of the NR structural gene from chicory is also presented. Southern blot analysis revealed the presence of a single nia gene per haploid genome of chicory.
Assuntos
Cichorium intybus/enzimologia , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Nitrato Redutases/genética , Nitratos/farmacologia , Linhagem Celular , Cichorium intybus/efeitos dos fármacos , Nitrato Redutase , Raízes de Plantas/efeitos dos fármacos , Raízes de Plantas/enzimologiaRESUMO
Two genomic clones corresponding to three members of the 1-aminocyclopropane-1-carboxylic acid (ACC) synthase gene family in potato (Solanum tuberosum L.) have been isolated and sequenced. Two highly homologous genes, ST-ACS1A and ST-ACS1B, transcribed in opposite directions were found in an 8.9 kb region. Their coding sequences are interrupted by two introns at identical positions. Their closest relative in tomato is the LE-ACS3 gene. The third gene in potato, ST-ACS2, was found in a 4 kb region and shows a gene structure similar to that of the tomato LE-ACS4 gene and to the mung bean VR-ACS4 and VR-ACS5 genes. Based on its lack of significant homology to the tomato gene family and its closeness to the VR-ACS4 and VR-ACS5 genes, we propose that LE-ACS7 represents an additional isoform in the tomato genome. Moreover, in a phylogenetic comparison of known ACC synthases, the ST-ACS2 isoform was grouped in a separate lineage together with the mung bean VR-ACS4 and VR-ACS5, and the moth orchid DS-ACS1A and DS-ACS1B gene products. Expression of the three potato genes was studied by reverse transcription-polymerase chain reaction on total RNA. The twin genes are positively regulated by indole-3-acetic acid in hypocotyls and expression is modulated by wounding in the leaves. The third gene is responsive to ethylene and wounding mainly in tubers. The roles of these three genes and of other members of the ACC synthase gene family in vegetative processes of potato such as tuberization, dormancy, and sprouting have yet to be determined.