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Am J Bot ; 98(4): e81-3, 2011 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-21613153

RESUMO

PREMISE OF THE STUDY: The first microsatellite primers were developed for Solanum centrale, a native Australian species used in the bush foods industry. Existing markers developed for other Solanum species were also amplified. METHODS AND RESULTS: Using an enrichment cloning protocol, seven novel markers were developed, and 48 existing markers from other Solanum species were tested, resulting in the characterization of a set of six highly polymorphic co-dominant loci for use in S. centrale. Microsatellite screening revealed polyploidy. Among the six highly polymorphic loci, allelic diversity ranged from 7 to 14. CONCLUSIONS: These markers will be useful for investigating genetic diversity and as a simple way of estimating ploidy of wild populations.


Assuntos
Primers do DNA , DNA de Plantas/análise , Loci Gênicos , Repetições de Microssatélites , Polimorfismo Genético , Poliploidia , Solanum/genética , Alelos , Austrália , Clonagem Molecular , Dieta , Ecossistema , Especificidade da Espécie
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