Parametric modeling with beamspread compensation and MIMO frequency domain inversion applied to fine saturated sands.

A system identification technique is applied to estimate the intrinsic absorption and dispersion of two fine sands. The method is based on the parametric modeling of the wave propagation through a Plexiglas tank filled with the sediment under investigation. The applicability of various porous models is discussed. The viscoelastic constant Q model and viscoelastic rational form model are applied and compared. Closed form expressions describing the wave propagation are replaced by Debye series expansions with correction coefficients that consider the beamspread caused by the finite aperture of the emitter. A multiple input multiple output (MIMO) representation is used in conjunction with the maximum likelihood estimator (MLE) for the estimation of the sediment parameters. The estimated absorption and dispersion curves are depicted.

Experimental validation for the diffraction effect in the ultrasonic field of piston transducers and its influence on absorption and dispersion measurements.

The aim of this work was to study the diffraction effects in the ultrasonic field of piston source transducers and their importance for accurate measurements of attenuation and dispersion in viscoelastic materials. In laboratory measurements, the diffraction phenomena are mainly due to the beam spread of the ultrasonic wave propagating in viscoelastic materials. This effect is essentially related to the estimated attenuation and dispersion in the material. In this work, a frequency domain system identification approach, using the maximum likelihood estimator (MLE), was applied to the measured data in order to determine a function for correcting the diffraction losses in both normal and oblique incidences for a large frequency band (300 kHz to 3 MHz). The effective radius of the used transmitter was determined by the inverse problem when ultrasonic beam propagation was investigated in a water medium. Using the estimated radius, the propagation through viscoelastic materials was established, and the acoustic parameters of these materials were estimated. Attention was paid to the determination of the attenuation and dispersion in the materials. These quantities were compared to those obtained without diffraction correction in order to see the influence of introducing the diffraction correction into the propagation model.

A frequency domain inversion method applied to propagation models in unconsolidated granular materials

The measurement setup consists of a water filled tank within which a smaller Plexiglas tank containing a known sediment is placed. This provides a Plexiglas-sediment-Plexiglas configuration, which is then used in conjunction with transducers and a pulser-receiver for a transmission and reflection experiment. The aim of this work is to find some physical parameters of the sediment from these measurements. A general viscoelastic model that considers losses due to absorption and dispersion along the propagation of the wave through the sediments is proposed. A rational transfer function is used to model the viscoelastic modulus of the bulk sediments, and comparisons with constant Q viscoelastic modelling and Biot modelling are performed. The estimation of the model parameters is elaborated using a maximum likelihood estimator in the frequency domain.

Experimental investigation of bounded beam reflection from plane interfaces in the vicinity of leaky waves angles

The aim of this work is an experimental validation of the reflected and transmitted fields through a viscoelastic material when a bounded ultrasonic beam is incident at different angles. Special measurements are carried out when incidence is performed at some critical angles of the material used. So the propagation of leaky waves along the solid layer is investigated. The inverse procedure for optimizing model parameters from the noisy input-output data is performed using the maximum likelihood estimator.

Trimodality of isoniazid elimination: phenotype and genotype in patients with tuberculosis.

The study was undertaken to show that polymorphic isoniazid elimination in humans is trimodal; that the acetylator genotype and eliminator phenotype of the individual patient are concordant; and that the differences in the pharmacokinetic parameters of fast, intermediate, and slow eliminator subgroups are statistically significant. Sixty adult patients of both sexes and of mixed race with tuberculosis participated in the trial. The apparent elimination rate constant (k, h(-1)) and the area under the isoniazid concentration-time curve (AUC, mg/L/h), over the interval 2 to 6 h after oral isoniazid were determined in all patients; NAT2 allele composition was determined in 47 patients. Serum INH concentrations were determined by HPLC and genotypes by PCR/restriction enzyme analysis. Three eliminator phenotypes could be distinguished, and concordance between the phenotype and the genotype of the individual could be demonstrated. The isoniazid concentration-time profiles of the three eliminator subgroups were significantly different (p < 0.05). The NAT2*12A allele, which codes for fast acetylation, has a high frequency in the population studied, the intermediate acetylator genotype is constituted of codominant fast and slow alleles, and the distribution of phenotypes/genotypes in the population is consistent with Hardy-Weinberg predictions. The therapeutic implications of polymorphic isoniazid metabolism are discussed.

Analysis of co-transfected expression vectors amplified by methotrexate selection in rat-1 cells.

In an earlier investigation of the influence of high level expression of p21H-ras, rat-1 cells were co-transfected with a selectable vector (pSV2Neo), an amplifiable vector (encoding dihydrofolate reductase; DHFR) and an H-ras expression vector. In this study we have analyzed the gene dose and expression levels of the three co-transfected plasmid vectors in cell lines that had been selected and isolated at different methotrexate concentrations. Growth of the cells in the absence of selection and Southern blot analyses indicate that the transfected vectors are stably co-integrated into the host genome. High expression levels from all three co-transfected vectors were evident at both the mRNA and protein levels, indicating that they are tightly linked in the host genome. The presence of a large amount of unspliced H-ras mRNA in cells expressing high levels of H-ras p21 indicates that processing of mRNA may be rate-limiting. Comparison of the gene dose and expression levels shows that the resistance of cells to increased methotrexate concentrations can occur by different mechanisms. It is concluded that co-transfection of individual plasmid vectors into rat-1 cells, followed by methotrexate selection, is an effective manner of achieving high level expression of proteins in cultured cells.

Increased intracellular Ca2+ is necessary for maximal expression of the proto-oncogene c-jun in the Jurkat T-cell line.

The time course and signal-transduction requirements for proto-oncogene c-jun expression in T-cells were investigated. Expression of c-jun mRNA was evident at 30 min after stimulation. Both the activation of Ca2+/phospholipid-dependent kinase as well as an increased intracellular free Ca2+ concentration were necessary for the maximal induction of c-jun mRNA and synthesis of Jun protein 1 h after stimulation.

DNA screening for hereditary goitre in Afrikander cattle.

DNA isolated from blood samples of Afrikander cattle (n = 66) were screened by blot-hybridisation analysis to determine the frequency of carriers of the goitre mutation. No carriers were found, suggesting that the frequency of the goitre mutation is less than 0.01.

Cholera toxin partially inhibits the T-cell response to phytohaemagglutinin through the ADP-ribosylation of a 45 kDa membrane protein.

This study examines the influence of cholera toxin (CT) on T lymphocyte activation by the mitogenic lectin phytohaemagglutinin (PHA). CT suppressed lectin-induced [3H]thymidine uptake in a dose-dependent fashion and acted synergistically with PHA in the generation of intracellular cyclic AMP. The toxin was assumed to act on Gs, because it also stimulated ADP-ribosylation of a 45 kDa membrane protein in vitro; no additional substrates were seen. The inhibitory effect of the adenylate cyclase/cyclic AMP pathway was shown to be directed at a concomitant stimulatory pathway, namely inositol phospholipid turnover. Lectin-stimulated 32P incorporation into both phosphatidylinositol as well as its 4,5-biphosphate derivative was depressed in the presence of CT or exogenous dibutyryl cyclic AMP. This, in turn, was associated with reduced activation of C-kinase as determined by decreased lectin-induced translocation from the cytosol to the surface membrane. These results indicate that Gs probably acts as a transducer between the PHA receptor and adenylate cyclase and may give rise to an exaggerated adenylate cyclase response in the presence of CT. It would seem as if reduction in inositol phospholipid turnover is related to the elevation of cyclic AMP rather than a CT effect on a putative transducer which acts directly on phospholipase C. Our study does not exclude the existence of non-CT-sensitive transducers in this capacity.

Characterization of a new alpha zero thalassaemia defect in the South African population.

A new alpha thalassemia defect has been detected in the South African population. Restriction mapping of the alpha globin gene cluster in affected individuals has established that the defect is associated with the removal of 22.8-23.7 kb of DNA, including the psi zeta 1, psi alpha 1, psi alpha 2, alpha 2 and alpha 1 globin genes. The 5' endpoint of the deletion has been localized between the zeta 2 and psi zeta 1 globin genes, and the 3' endpoint lies 4-5 kb 3' to the alpha 1 globin gene. We have called the deletion - -SA in order to distinguish it from alpha zero thalassaemia defects described in other populations.

Polymorphism of DNA sequence in the human pro alpha 2(I) collagen gene.

The human pro alpha 2(I) collagen gene was analysed for the presence of restriction fragment length polymorphisms. DNA from randomly selected unrelated persons of three Southern African populations was cleaved with one of eight different restriction enzymes, electrophoresed, blotted, and hybridised with cDNA and genomic probes specific for the pro alpha 2(I) gene. An MspI polymorphism was detected which results from the loss of a cleavage site within the 3' half of the gene. In two of the populations studied, the polymorphism occurred at significant frequencies, and should therefore prove useful as a genetic marker for the study of inherited disorders of connective tissue involving collagen structure or biosynthesis.

Afrikander cattle congenital goiter: size heterogeneity in thyroglobulin mRNA.

The mRNA coding for thyroglobulin in cattle homozygous for an autosomal recessive defect of thyroglobulin synthesis was investigated using a recombinant plasmid containing bovine mRNA coding sequence. Total RNA preparations from goiter contained one third of the thyroglobulin mRNA sequences found in normal thyroid tissue. This mRNA was not translated into thyroglobulin by Xenopus oocytes. Northern transfer analysis revealed both a normal sized and a smaller thyroglobulin mRNA in the goiter.

Characterization of the messenger RNA population coding for components of viperid snake venom.

Total polyadenylated messenger RNA was prepared from the milked venom glands of the South African puff adder (Bitis arietans) and translated in an in vitro translation system. The products of cell free synthesis were immunoprecipitable with puff adder venom antiserum. Treatment of these cell free products with a dog pancreas microsomal membrane preparation demonstrated the presence of signal peptides. Northern blot hybridization of total puff adder venom gland mRNA to its complementary single stranded copy DNA revealed two discrete mRNA populations coding for the major components of puff adder venom. The relative amounts of these polyadenylated mRNA sequences changed during the onset of venom synthesis, suggesting mRNA deadenylation, general endonucleolytic RNA degradation and selective degradation of high molecular weight message components.

Blot hybridisation analysis of genomic DNA.

Restriction endonuclease analysis of specific gene sequences is proving to be a valuable technique for characterisation and diagnosis of inherited disorders. This paper describes detailed protocols for isolation, restriction, and blot hybridisation of genomic DNA. Problems and alternatives in the procedure are discussed and a troubleshooting guide has been provided to help rectify faults.