RESUMO
Several species of Cryptosporidium have been associated with infection. Cryptosporidium parvum and Cryptosporidium hominis are the main agents of cryptosporidiosis in humans. Stool samples from 108 Cryptosporidium-infected patients were submitted to PCR-RFLP analysis for a 553-bp fragment of Cryptosporidium oocyst wall protein (COWP) gene and an 826-864 bp fragment of the small-subunit ribosomal RNA (SSU-rRNA) gene. Ninety-two patients were immunocompetent children and 16 were HIV-infected adults. C. hominis was detected in 69 patients (59 immunocompetent and 10 HIV-infected); C. parvum, in 34 patients (28 immunocompetent and 6 HIV-infected); and C. meleagridis and C. felis in one patient each (both immunocompetent children). Three samples yielded negative results. C. parvum was significantly more frequent in children from rural areas than in those of urban residence (p=0.010). As far as we know, this is the first surveillance study about the molecular characterization of Cryptosporidium in humans performed in Spain. The finding of zoonotic species infecting humans calls for further research on this subject.
Assuntos
Criptosporidiose/epidemiologia , Criptosporidiose/parasitologia , Cryptosporidium/classificação , Cryptosporidium/genética , Genes de RNAr , Proteínas de Protozoários/genética , Infecções Oportunistas Relacionadas com a AIDS/epidemiologia , Infecções Oportunistas Relacionadas com a AIDS/parasitologia , Adulto , Animais , Criança , Pré-Escolar , Criptosporidiose/complicações , Cryptosporidium/isolamento & purificação , Cryptosporidium parvum/classificação , Cryptosporidium parvum/genética , Cryptosporidium parvum/isolamento & purificação , Fezes/parasitologia , Feminino , Infecções por HIV/complicações , Infecções por HIV/epidemiologia , Infecções por HIV/parasitologia , Humanos , Incidência , Lactente , Masculino , Oocistos/química , Reação em Cadeia da Polimerase , Polimorfismo de Fragmento de Restrição , Vigilância da População , População Rural , Espanha/epidemiologia , Especificidade da Espécie , População UrbanaAssuntos
Criptosporidiose/epidemiologia , Cryptosporidium , Animais , Gatos , Pré-Escolar , Criptosporidiose/parasitologia , Cryptosporidium/classificação , Cryptosporidium/genética , Cryptosporidium/isolamento & purificação , Cryptosporidium/patogenicidade , Humanos , Imunocompetência , Índia , Masculino , Espanha/epidemiologia , ViagemRESUMO
Pityriasis versicolor is a superficial infection of the stratum corneum by several yeast species formerly collectively named Malasseziafurfur. The genus Malassezia has been recently enlarged with new species. With the exception of M. pachydermatis, the remaining six species have an absolute requirement in vitro for supplementation of long-chain fatty acids in media. These lipophilic yeasts comprise six species: M. furfur, M. globosa, M. obtusa, M. restricta, M. slooffiae and M. sympodialis. The aim of this study was to establish whether there was any association between the various species of Malassezia and pityriasis versicolor lesions. Thus, we studied the isolates from 79 patients with pityriasis versicolor, both from lesions and from apparently healthy skin close to the visible lesions. In pityriasis versicolor lesions, the species most frequently isolated was M. globosa (90%), followed by M. sympodialis (41%). Almost all isolates (99%) belonged to one of these two species. The most frequent pattern was M. globosa as the sole species (58% of cases), although the association with M. sympodialis was also frequent (30%). These results confirmed M. globosa as the main agent of pityriasis versicolor and M. sympodialis as the second agent in importance. Malassezia globosa was found to be a species with high levels of esterase and lipase enzymes of probable importance in their pathogenicity.
Assuntos
Malassezia/isolamento & purificação , Tinha Versicolor/microbiologia , Adolescente , Adulto , Idoso , Esterases/metabolismo , Feminino , Humanos , Lipase/metabolismo , Malassezia/enzimologia , Masculino , Pessoa de Meia-Idade , Espanha , beta-Glucosidase/metabolismoAssuntos
Criptosporidiose/transmissão , Cryptosporidium parvum/genética , Cryptosporidium parvum/isolamento & purificação , Moscas Domésticas/microbiologia , Insetos Vetores/microbiologia , Animais , Bovinos , Criptosporidiose/epidemiologia , Primers do DNA/análise , Fezes/microbiologia , Humanos , Oocistos/isolamento & purificação , Reação em Cadeia da Polimerase/métodos , Prevalência , RNA Ribossômico 18S/análiseRESUMO
A total of 102 Hafnia alvei clinical strains isolated from different patients with diarrhea has been tested, using polymerase chain reaction and dot-blot hybridization, for the enteropathogenic Escherichia coli attaching and effacing A (eaeA) gene to establish their role as a causative agent of diarrhea in our environment. None of them was positive for the eaeA gene. We cannot consider the eaeA gene as the virulence-associated factor implicated in the H. alvei strains isolated from diarrheal feces in our region (AU)
No disponible
Assuntos
Humanos , Adesinas Bacterianas/genética , Hafnia alvei/isolamento & purificação , Proteínas de Transporte/genética , Diarreia/microbiologia , Infecções por Enterobacteriaceae/microbiologia , Espanha/epidemiologia , Genes Bacterianos , Hibridização de Ácido NucleicoRESUMO
No disponible
