RESUMO
A high proportion of human recurrent spontaneous abortions (RSA) remain unexplained. The possible association between RSA and different genetic polymorphisms within the human leucocyte antigen system (HLA system, the human major histocompatibility complex) has been investigated with conflicting results since many decades. Here, we describe a case-control study with 136 Southern Brazilian women of predominantly European ancestry (75 control and 61 cases with unexplained RSA). We investigated the relationship between unexplained RSA and alleles and genotypes from two classical loci of the HLA: HLA-DRB1 and HLA-DQB1, as well as three loci related to cytokine production and their serum levels: TNFA (-308G>A), IL10 (-1082G>A, -819T>C, -592A>C) and IFNG (+874A>T). Genotyping was performed by an allele-specific PCR method. While all results concerning cytokine-related genes turned out to be negative, we found the genotype HLA-DQB1*02:02, 03:01 to be significantly decreased and the allele HLA-DRB1*11:04 to be significantly increased among patients.
Assuntos
Aborto Habitual/genética , Antígenos de Histocompatibilidade Classe II/genética , Polimorfismo Genético , Aborto Habitual/metabolismo , Adulto , Brasil , Estudos de Casos e Controles , Citocinas/genética , Citocinas/metabolismo , Feminino , Frequência do Gene , Genótipo , Cadeias beta de HLA-DQ/genética , Cadeias beta de HLA-DQ/metabolismo , Cadeias HLA-DRB1/genética , Cadeias HLA-DRB1/metabolismo , Humanos , Fenótipo , GravidezRESUMO
Here we report the discovery of a novel HLA-B allele, named B*4212 in a Brazilian volunteer bone marrow donor. The new sequence has nucleotide variation at position 496 (TâG) as compared with B*4201. This variation results in a conservative amino acid substitution from valine to glycine at codon 165 of exon 3.
Assuntos
Antígenos HLA-B/genética , Alelos , Sequência de Aminoácidos , Substituição de Aminoácidos , Sequência de Bases , Medula Óssea , Brasil , Antígenos HLA-B/química , Antígenos HLA-B/imunologia , Teste de Histocompatibilidade , Humanos , Dados de Sequência Molecular , Mutação Puntual , Análise de Sequência de DNA , Doadores de TecidosRESUMO
A novel human leukocyte antigen-B allele named B*18:35 was identified in a Brazilian volunteer bone marrow donor.
Assuntos
Alelos , Medula Óssea , Antígenos HLA-B/genética , Doadores de Tecidos , Sequência de Bases , Brasil , Antígeno HLA-B18 , Teste de Histocompatibilidade , Humanos , Dados de Sequência Molecular , Alinhamento de SequênciaRESUMO
We evaluate a 32 hours-workshop on Primary Health Care. The method of the workshop was active participation and the tasks were: a) previous anonymous evaluation of knowledge, b) team work in 5-6 individuals teams in order to answer some practical problems, c) communication of results and synthesis with the whole group, and d) a second anonymous evaluation of knowledge (the same as the initial one) and an assessment of the perceived usefulness of the workshop. We present results on the modification of knowledge of the 237 students (96.2% of the students improve it), the usefulness of the workshop (91.1% consider it useful) and the evaluation of the methodology. This kind of teaching methodology is useful for stimulating the critical capacity of the students, improving their knowledge.