[Allelic polymorphism and analysis of haplotypes of the muscle protein kinase genes and haplotype analysis in residents of northwestern Russia and patients with myotonic dystrophy]. / Izuchenie allel'nogo polimorfizma i analiz gaplotipov gena myshechnoi proteinkinazy i zhitelei severo-zapadnogo regiona Rossii i u bol'nykh miotonicheskoi distrofiei.

The distribution of alleles with various CTG-repeat numbers was studied and the haplotypes for polymorphic sites HhaI and HinfI of mouse muscle protein kinase (DMPK) were analyzed in inhabitants of northwestern Russia and in patients with myotonic dystrophy (90 and 18 chromosomes, respectively). Twelve normal alleles with the triplet-repeat number from 5 to 24 were identified and the alleles with five (42.5%) and 11-13 (37%) repeats were found to be predominant. The bimodal distribution revealed is similar to those described earlier for other populations, however, the frequencies of individual alleles differed from those in populations of Europe and Central Russia. No significant differences in frequencies of CTG alleles were found in 32 normal chromosomes involved in compounds with the mutant chromosomes (i.e., in patients with myotonic dystrophy) as compared to their frequencies in the population. However, almost all mutant chromosomes (16 of 18) had the same haplotype for intragenic polymorphic sites: HhaI-; HinfI+. This haplotype was also inherent in 91% of all chromosomes with CTG5 and all chromosomes with a CTG number more than 15. Possible evolution of chromosomes with different numbers of triplet repeats mediating their expansion and impairing the function are discussed.

[Significance of the degree of static tension in the development of fatigue and overstrain of the neuromuscular system in polishers employed in the glass industry]. / Znachenie velichii staticheskikh nagruzok v razvitii utomleniia i perenapriazheniia nervno-myshechnogo apparata shlifovshchits stekloizdelii.

The research data proposed proved the key role of the muscles' static tension in the process of fatiguability and strain growth in glass-work polishers' neuromuscular mechanisms. It was proved that a comparatively greater static load on the shoulder girdle muscles in glass-work polishers, as compared to crystal-polishers, results in more vivid pathologic changes in this professional group. The research served as a basis for a set of health-related measures, including both palliative and radical approaches.

[Metabolic study of sarcoma 45 and Walker carcinosarcoma cells during the process of growth and exposure to anticoagulants (an enzyme cytochemical analysis)]. / Issledovanie metabolizma kletok sarkomy 45 i kartsinosarkomy Uoker v protsesse ikh rosta i pri antikoaguliantnom vozdeistvii (énzimotsitokhimicheskii analiz).

The enzyme activities of lactate dehydrogenase (LDH), succinate dehydrogenase (SDH) and acid phosphatase (AP) in the rat sarcoma 45 (S. 45) cells and Walker carcinosarcoma (WCS) cells were estimated from the histochemical study. No significant changes were found in the histochemical reaction intensity during the tumour growth but at the late stage of WCS growth the LDH activity increased. WCS metastases were distinguished by the elevated enzyme activity of LDH as compared with the tumour. During the anticoagulant treatment the LDH and SDH activity in S. 45 and WCS cells falls, but the AP activity increases.

[Metabolic study of sarcoma 45 and Walker carcinosarcoma cells during growth and anticoagulant exposure. An autoradiographic study]. / Issledovanie metabolizma kletok sarkomy 45 i kartsinosarkomy Uoker v protsesse ikh rosta i pri antikoaguliantnom vozdeistvii. Avtoradiograficheskoe issledovanie.

Intensity of DNA, RNA and protein synthesis was estimated autoradiographically in rat tumour cells of sarcoma 45 (S. 45) and Walker carcinosarcoma (WCS). It is shown that the DNA synthesis reduces with tumour growth, the RNA synthesis in growing S.45 increases. The level of nucleic acid synthesis is higher in metastatic cells of WCS than in the initial transplant. DNA, RNA and protein synthesis in neoplastic cells decreases under anticoagulant treatment.