RESUMO
This paper studies the effect of plant peptides of thionine Ns-W2 extracted from seeds of fennel flower (Nigella sativa) and ß-purothionine from wheat germs (Triticum kiharae), as well as a synthetic antimutagen (crown-compound), on the expression of several genes involved in the.control of cellular homeostasis, processes of carcinogenesis, and radiation response in human rhabdomyosarcoma cells (RD cells), T-lymphoblastoid cell line Jurkat, and blood cells. All of these agents acted as antimutagens-anticarcinogens, reducing the expression of genes involved in carcinogenesis (genes of families MMP, TIMP, and IAP and G-protein genes) in a tumor cell. A pronounced reduction in the mRNA level of these genes was caused by thionine Ns-W2, and the least effect was demonstrated by ß-purothionine. Antimutagens had very little effect on the mRNA levels of the several studied genes in normal blood cells.
Assuntos
Antimutagênicos/administração & dosagem , Peptídeos/administração & dosagem , Fenotiazinas/administração & dosagem , Extratos Vegetais/administração & dosagem , Rabdomiossarcoma/genética , Antimutagênicos/química , Carcinogênese/efeitos dos fármacos , Linhagem Celular Tumoral , Proliferação de Células/efeitos dos fármacos , Regulação Neoplásica da Expressão Gênica/efeitos dos fármacos , Humanos , Nigella sativa/química , Peptídeos/química , Fenotiazinas/química , Extratos Vegetais/química , Radiação Ionizante , Rabdomiossarcoma/tratamento farmacológico , Rabdomiossarcoma/patologia , Triticum/química , Proteína Supressora de Tumor p53/biossínteseAssuntos
Antimutagênicos/farmacologia , Benzodiazepinas/farmacologia , Regulação Neoplásica da Expressão Gênica , Linfócitos/metabolismo , Rabdomiossarcoma/metabolismo , Linhagem Celular Tumoral , Humanos , Proteínas Inibidoras de Apoptose/genética , Proteínas Inibidoras de Apoptose/metabolismo , Linfócitos/efeitos dos fármacos , Metaloproteinases da Matriz/genética , Metaloproteinases da Matriz/metabolismo , MicroRNAs/genética , MicroRNAs/metabolismo , NF-kappa B/genética , NF-kappa B/metabolismo , Inibidor Tecidual de Metaloproteinase-1/genética , Inibidor Tecidual de Metaloproteinase-1/metabolismo , Inibidor Tecidual de Metaloproteinase-2/genética , Inibidor Tecidual de Metaloproteinase-2/metabolismoRESUMO
Antimutagenic effects of polypeptides isolated from Triticum kiharae wheat plantule extracts have been studied on human cells exposed to cadmium chloride. The most effective polypeptide Tk-AMP-BP ß -purothionin exhibited higher antimutagenic activity than wheat water extract and another peptide isolated from the same wheat species, Tk-AMP-γ 2 defensin; it also produced a pronounced antioxidant effect. This polypeptide can be used as a preventive agent for reducing the mutagenic potential of some environmental pollutants and for correction of human diseases associated with the defense system defects.
Assuntos
Peptídeos Catiônicos Antimicrobianos/farmacologia , Antimutagênicos/farmacologia , Antioxidantes/farmacologia , Extratos Vegetais/farmacologia , Proteínas de Plantas/farmacologia , Triticum/química , Cloreto de Cádmio/efeitos adversos , Células Cultivadas , Defensinas/farmacologia , Humanos , Medições Luminescentes , Linfócitos/efeitos dos fármacosAssuntos
Antimutagênicos/farmacologia , Transcrição Gênica/efeitos dos fármacos , Peptídeos Catiônicos Antimicrobianos/farmacologia , Estudos de Casos e Controles , Síndrome de Down/genética , Síndrome de Down/metabolismo , Glutationa Peroxidase/genética , Glutationa Peroxidase/metabolismo , Proteínas de Choque Térmico/genética , Proteínas de Choque Térmico/metabolismo , Humanos , Linfócitos/efeitos dos fármacos , Linfócitos/metabolismo , Taxa de Mutação , Proteínas Nucleares/genética , Proteínas Nucleares/metabolismo , Nucleofosmina , Proteínas Oncogênicas/genética , Proteínas Oncogênicas/metabolismo , Proteínas de Plantas/farmacologia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Superóxido Dismutase/genética , Superóxido Dismutase/metabolismoRESUMO
The mutagenic activity of thallium carbonate and mercury chloride was estimated by the HAP chromatography and virus reactivation methods in cultures of embryo cells of mice (CBA and C57Bl/6 strains) and rats and by the dominant mutation frequency in rats. Thallium carbonate induced single-stranded DNA breaks. The induction of DNA breaks correlated with the rate of virus reactivation and the mutability of vaccinia virus in the cell cultures studied. DNA breaks in experiments with mercury chloride occurred at much lower concentrations as compared with these of thallium carbonate. The rate of vaccinia virus reactivation in cells treated with mercury chloride was reduced, whereas the level of virus mutagenesis did not differ from the control. In the dominant lethal test the mutagenic activity of thallium carbonate was higher than the mutagenic activity of mercury chloride.
Assuntos
Reparo do DNA , Mercúrio/toxicidade , Tálio/toxicidade , Animais , Células Cultivadas , Genes Dominantes , Camundongos , Testes de Mutagenicidade , Mutação/efeitos dos fármacos , Ratos , Vaccinia virus/efeitos dos fármacos , Vaccinia virus/genéticaRESUMO
This work was devoted to investigation or repair regulation by biological factors: viruses and interferon. DNA damage induced by gamma- and UV-irradiation, ethyleneimine and 4-nitro-quinoline-1-oxide (4-NQO) were studied, by sedimentation of lysed cells through alkaline sucrose gradients, by hydroxylapatite column chromatography and by the chromosomal aberration test. The reproducible vaccinia virus resulted in simulation repair activity of chick embryo cells after treatment with 4-NQO. Interferon, added after gamma- and UV-irradiation, decreased the chromosomal aberration level, stabilized it after ethyleneimine treatment and also stimulated the ability of cells to rejoin DNA breaks induced by 4-NQO. The cause of this phenomenon is discussed.