RESUMO
Teichoic acids of lactic acid bacteria probiotic strains have been obtained by extraction from native cells, followed by purification of extracts using ion exchange chromatography. Selected fractions contained high concentrations of phosphorus and did not contain nucleic acids. The content of teichoic acid depended on the species and strain specificity. Heterogeneity of the studied biomolecules was revealed.
Assuntos
Parede Celular/química , Enterococcus faecium/metabolismo , Lactobacillus plantarum/metabolismo , Ácidos Teicoicos/isolamento & purificação , Cromatografia por Troca Iônica , Enterococcus faecium/isolamento & purificação , Ácido Láctico/biossíntese , Lactobacillus plantarum/isolamento & purificação , Fósforo/análise , Probióticos/química , Especificidade da EspécieRESUMO
The spot sweetflag virus (SSV) as to its morphology and structural components in comparison with phytorhabdovirus of curly potato dwarf virus (CPDV) and rhabdoviruses of vesicular stomatitis virus (VSV), pathogenic for people and animals, corresponds to the definition of rhabdovirus and belongs to Rhabdoviridae family. Virions of SSV have a bacillus-like form and dimensions 110-130 x 45 nm. SSV contains structural proteins 130, 66, 43-39, 32-30 and 25 kDa. In the virion structure the fatty acids have been identified: palmitic (47 %), linolic (4.2%), oleic (14.9%), stearic (3.94%), holesterol (23%), and also carbohydrates: glucose (25.3%), galactose (18.3%), arabinose (16%), rhamnose (3.1%) and mannose (2.32%). Aminosaccharides: glucosamine and galactosamine, with correlation 1:7.2, were also found out. The paper is presented in Ukrainian.