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1.
Sci Data ; 11(1): 891, 2024 Aug 16.
Artigo em Inglês | MEDLINE | ID: mdl-39152143

RESUMO

Paspalum notatum Flüggé is an economically important subtropical fodder grass that is widely used in the Americas. Here, we report a new chromosome-scale genome assembly and annotation of a diploid biotype collected in the center of origin of the species. Using Oxford Nanopore long reads, we generated a 557.81 Mb genome assembly (N50 = 56.1 Mb) with high gene completeness (BUSCO = 98.73%). Genome annotation identified 320 Mb (57.86%) of repetitive elements and 45,074 gene models, of which 36,079 have a high level of confidence. Further characterisation included the identification of 59 miRNA precursors together with their putative targets. The present work provides a comprehensive genomic resource for P. notatum improvement and a reference frame for functional and evolutionary research within the genus.


Assuntos
Genoma de Planta , Anotação de Sequência Molecular , Paspalum , Paspalum/genética , Cromossomos de Plantas/genética , MicroRNAs/genética , Sequências Repetitivas de Ácido Nucleico
2.
New Phytol ; 240(2): 597-612, 2023 10.
Artigo em Inglês | MEDLINE | ID: mdl-37548040

RESUMO

Here, we report the characterization of a plant RNA methyltransferase, orthologous to yeast trimethylguanosine synthase1 (Tgs1p) and whose downregulation was associated with apomixis in Paspalum grasses. Using phylogenetic analyses and yeast complementation, we determined that land plant genomes all encode a conserved, specific TGS1 protein. Next, we studied the role of TGS1 in female reproduction using reporter lines and loss-of-function mutants in Arabidopsis thaliana. pAtTGS1:AtTGS1 reporters showed a dynamic expression pattern. They were highly active in the placenta and ovule primordia at emergence but, subsequently, showed weak signals in the nucellus. Although expressed throughout gametophyte development, activity became restricted to the female gamete and was also detected after fertilization during embryogenesis. TGS1 depletion altered the specification of the precursor cells that give rise to the female gametophytic generation and to the sporophyte, resulting in the formation of a functional aposporous-like lineage. Our results indicate that TGS1 participates in the mechanisms restricting cell fate acquisition to a single cell at critical transitions throughout the female reproductive lineage and, thus, expand our current knowledge of the mechanisms governing female reproductive fate in plants.


Assuntos
Arabidopsis , Arabidopsis/metabolismo , Saccharomyces cerevisiae , Filogenia , Mutação/genética , Óvulo Vegetal/metabolismo , Células Germinativas , Regulação da Expressão Gênica de Plantas
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