Disperse red 15 (DR15) impedes biofilm formation of uropathogenic Escherichia coli.

Catheter associated urinary tract infection (CAUTI) is a highly prevalent hospital-acquired infection that is predominantly caused by uropathogenic Escherichia coli (UPEC). It adheres on catheter surface using type I pili as the initial step of pathogenesis that progresses to form biofilm. In this study, potential inhibitors against FimH adhesin of type I pili were screened computationally that yielded ten compounds. These were further validated in vitro against adhesion and biofilm formation. The compounds, 1-Amino-4-hydroxyanthraquinone (Disperse Red 15 or DR15) and 4-(4'-chloro-4-biphenylylsulfonylamino) benzoic acid (CB1) impaired adhesion and biofilm formation without inhibiting the planktonic growth. Also, both compounds inhibited cell assemblages like autoaggregation and swarming motility by unknown mechanisms. DR15 was further derivatised into N-(4-hydroxy-9,10-dioxo-9,10-dihydroanthracen-1-yl) undec-10-enamide that self-assembled with linseed oil, which was used as the coating material on urinary Foley catheters. The thin-film coating on the catheter did not leach when incubated in artificial urine and effectively restricted biofilm formation of UPEC. Altogether, the thin-film coating of urinary catheter with DR15 inhibited biofilm formation of UPEC and this application could potentially help to reduce CAUTI incidents in healthcare facilities.

Crystal engineering with heteroboranes. II. 1,2-Dicarboxy-1,2-dicarba-closo-dodecaborane(12) ethanol hemisolvate.

The title compound, 1,2-(COOH)(2)-1,2-closo-C(2)B(10)H(10).0.5C(2)H(6)O or C(4)H(12)B(10)O(4).0.5C(2)H(6)O, forms a tetramer by incorporating ethanol (solvent) molecules through hydrogen bonding. Two eight-membered rings [graph set R(2)(2)(8)] are formed by hydrogen bonding between two carboxylic acid groups, whereas two ten-membered rings [R(3)(3)(10)] are formed by hydrogen bonding between two carboxylic acid groups and the OH group of an ethanol molecule (solvent). Two crystallographically independent tetramers are present in the crystal structure.

Crystal engineering with heteroboranes. III. 2-Carboxy-1-methoxymethyl-1,2-dicarba-closo-dodecaborane(12).

The title compound, 1-CH(2)OCH(3)-2-COOH-1,2-closo-C(2)B(10)H(10) or C(5)H(16)B(10)O(3), forms a discrete centrosymmetric tetramer, via hydrogen bonding, involving two inner and two outer carborane molecules. One conventional eight-membered hydrogen-bonded ring [graph set R(2)(2)(8)] is formed between two carboxylic acid groups of the inner carboranes. This interaction is then supplemented by an open finite hydrogen bond (graph set D) between the ether O atom of the inner carborane and the carboxylic acid H atom of the outer carborane.