To spread or not to spread? Assessing the suitability of sewage sludge and other biogenic wastes for agriculture reuse.

Sewage sludge (biosolids) management represents a worldwide issue. Due to its valuable properties, approximately one half of the EU production is recovered in agriculture. Nevertheless, growing attention is given to potential negative effects deriving from the presence of harmful pollutants. It is recognized that a (even very detailed) chemical characterization is not able to predict ecotoxicity of a mixture. However, this can be directly measured by bioassays. Actually, the choice of the most suitable tests is still under debate. This paper presents a multilevel characterization protocol of sewage sludge and other organic residues, based on bioassays and chemical-physical-microbiological analyses. The detailed description of the experimental procedure includes all the involved steps: the criteria for selecting the organic matrices to be tested and compared; the sample pre-treatment required before the analyses execution; the chemical, physical and microbiological characterisation; the bioassays, grouped in three classes (baseline toxicity; specific mode of action; reactive mode of action); data processing. The novelty of this paper lies in the integrated use of advanced tools, and is based on three pillars:•the direct ecosafety assessment of the matrices to be reused.•the adoption of innovative bioassays and analytical procedures.•the original criteria for data normalization and processing.

Innovations for Reducing Methane Emissions in Livestock toward a Sustainable System: Analysis of Feed Additive Patents in Ruminants.

An important challenge for livestock systems is the mitigation of environmental impacts while ensuring food security, and feed additives are considered as one of the most promising mitigation strategies. This study analyzed the innovation landscape of feed additives to reduce methane emissions in ruminants. The analysis is based on patent data to evaluate the development, scientific importance, and market-level impact of the innovations in this field. The results reveal that the EU is on the innovation frontier, with substantial and quality patent production. The innovation field is dominated by private players, characterized by high specificity in the R&D pipeline. Additives derived from plant or botanical extracts, together with 3-nitrooxypropanol (3-NOP), represent the emerging innovations, indicating a clear orientation toward more sustainable livestock systems. Despite the regulatory and semantic limitations related to the use of patent databases, data reveal a growing innovation activity at global level, which could lead to macroeconomic benefits for the entire livestock sector.

Global Innovation Trends for Plant-Based Vaccines Production: A Patent Analysis.

The use of plants as biofactories for the production of medical products and vaccines has a long history, but the recent COVID-19 pandemic has caused this set of technologies, for their potential to contribute to the development of innovative solutions for tackling pandemic spread worldwide, to rise in prominence. The purpose of this paper is to analyze the global innovation scenario of plant-based vaccine production. METHODS: Patent search using a specific set of technical classification codes and keywords was performed using the Questel-Orbit database, with a final output of 180 patent families, corresponding to 1397 single patents. RESULTS: Plant-based vaccines production is an innovation sector with positive development especially in the last five-year period (30% growth). Fifty percent of the patents were registered in the United States, standing out as the most attractive patent system worldwide. The inventive activity was led by private firms owning the 49% of the patent families, and the key-players group includes the companies that successfully developed plant-based COVID-19 vaccine candidates, indicating a strong connection between the expertise in innovation production and the capacity to adapt inventions to the current pandemic vaccine demand. Virus-like particles technology has increased in importance over the past few years. CONCLUSION: Patent data confirm their relevant role as indicators of innovation and technological evolution. Plant-based vaccines are expected to acquire an increasing role over the next few years as the current pandemic acts as an innovation catalyst.

RNA-based biocontrol compounds: current status and perspectives to reach the market.

Facing current climate challenges and drastically reduced chemical options for plant protection, the exploitation of RNA interference (RNAi) as an agricultural biotechnology tool has unveiled possible new solutions to the global problems of agricultural losses caused by pests and other biotic and abiotic stresses. While the use of RNAi as a tool in agriculture is still limited to a few transgenic crops, and only adopted in restricted parts of the world, scientists and industry are already seeking innovations in leveraging and exploiting the potential of RNAi in the form of RNA-based biocontrol compounds for external applications. Here, we highlight the expanding research and development pipeline, commercial landscape and regulatory environment surrounding the pursuit of RNA-based biocontrol compounds with improved environmental profiles. The commitments of well-established agrochemical companies to invest in research endeavours and the role of start-up companies are crucial for the successful development of practical applications for these compounds. Additionally, the availability of standardized guidelines to tackle regulatory ambiguities surrounding RNA-based biocontrol compounds will help to facilitate the entire commercialization process. Finally, communication to create awareness and public acceptance will be key to the deployment of these compounds. © 2019 Society of Chemical Industry.

Exploring the Patent Landscape of RNAi-based Innovation for Plant Breeding.

BACKGROUND: RNA interference (RNAi) is an innovative technique for plant improvement based on naturally occurring mechanisms which show great potential because of their high specificity and possibility to be applied through innovative methods of topical application in plants. This specific innovation sector is worth analysing from the economic perspective given the great potentiality of RNAi-based plants and products to support modern agriculture in reaching the goals for the improvement of agri-food chains global sustainability. This paper aims to evaluate the global landscape of RNAi innovation by analysing patent data as indicators of innovation output. METHODS: We revised all patents relating to RNAi in plants based on a dataset of roughly seven thousand patent families. The analysis classified inventions according to a set of variables able to characterise the dynamics of innovation (i.e. public/private ownership, type of plants involved, main traits) while the use of concentration indices provided insights into the evolution of this sector. RESULTS: Results revealed that RNAi is a technique with promising future applications, able to provide solutions to a great variety of agricultural issues and principally developed by the US and Chinese applicants, whereas European innovation capacity in this field appears to be limited. CONCLUSION: The innovation landscape of plant breeding is rapidly evolving and RNAi technique is probably going to play a major role in this field.

Consumers' Preferences for a Local Food Product: The Case of a New Carnaroli Rice Product in Lombardy.

Italy, with a cultivated area of 218,000 ha, is a European leader of rice production. In particular Lombardy region accounts for 40% of total rice cultivation and the case study in object accounts for 3.2% of Lombardy total rice area (2773 ha). Starting from 2012, through a regional project titled Buono, Sano e Vicino (good, healthy and close), Riso e Rane rural district supported local rice farmers in developing innovation in rice production and promoting an alternative supply chain to increase farmers bargaining power and promote new market strategies. More specifically, the innovation introduced is a new biotech method for variety certification, named DNA controllato (DNA tested). In the first step of the project, the attention was focused on an Italian traditional variety of rice: Carnaroli rice. Thanks to a commercial agreement with one of the most important large retailers in Lombardy, the Riso e Rane rice is offered for sale both in the traditional and wholegrain version. In this context, this work aims to evaluate the determinants of consumer's quality perception of this product, through a preference study of the commercial rice package. Preliminary results reveal that consumers perceive information about origin, local food-system and tradition more easily than DNA tested certification. In conclusion, this work contributes to evaluate the role of bio economy applications to the food sector and offers new insights for the debate about the relationships between tradition and innovation.

How scary! An analysis of visual communication concerning genetically modified organisms in Italy.

Several studies provide evidence of the role of written communication in influencing public perception towards genetically modified organisms, whereas visual communication has been sparsely investigated. This article aims to evaluate the exposure of the Italian population to scary genetically modified organism-related images. A set of 517 images collected through Google are classified considering fearful attributes, and an index that accounts for the scary impact of these images is built. Then, through an ordinary least-squares regression, we estimate the relationship between the Scary Impact Index and a set of variables that describes the context in which the images appear. The results reveal that the first (and most viewed) Google result images contain the most frightful contents. In addition, the agri-food sector in Italy is strongly oriented towards offering a negative representation of genetically modified organisms. Exposure to scary images could be a factor that affects the negative perception of genetically modified organisms in Italy.