RESUMO
A thermodynamic model is proposed to describe the melting of lamellar crystallite in a solid medium. This model includes a modification of the Gibbs-Thomson equation to make it applicable to the above-mentioned crystallites. The need for such modification is supported experimentally by studying the impact of the surroundings on the melting point of the crystallites. In particular, the application of the model to nanocrystals in open-porous systems makes it possible to determine the analytical relations for the melting point, the heat of melting, and the inverse effective size of the pores. The fitting of the experimental data with these functional relations then allows for the calculation of the nanocrystalline density, pressure in the nanocrystal, and difference in the surface tension coefficients at the nanocrystal-matrix interface and melt-matrix interface, as well as the difference in the surface entropies per unit area at the nanocrystal-matrix and melt-matrix interfaces.
RESUMO
Hepatitis C virus (HCV) strains were isolated from human sera in vitro. NGUK-1 rat neurinoma neural cells [1] were selected as the experimental model of HCV. The cells were infected after attaining the confluence. The virus caused no cytopathic effect during its multiplication. After monolayer infection (24-96 h), culture fluid samples were tested for HCV RNA by classical PCR with electrophoretic detection of the reaction products and by quantitative real time PCR. All samples from infected culture were positive, control samples (intact cultures) were negative. Coefficient of correlations in quantitative PCR was 0.99. The results are reliable, conform to the normal values for this series of the test system, and indicate that HCV is replicated in continuous NGUK-1 cells. This in vitro model can be used for isolation of HCV.
