Repercussões negativas e impacto psicológico da pandemia por Covid-19 nas equipes de saúde / Negative repercussions and psychological impact of pandemic by Covid-19 on health teams / Repercusiones negativas e impacto psicológico de la pandemia por Covid-19 en equipos de salud

Objetivo: identificar as repercussões negativas e o impacto psicológico em profissionais de saúde que atuam diretamente no cuidado aos pacientes com coronavírus e possíveis estratégias para minimizar seus efeitos. Método: trata-se de uma revisão integrativa, com busca de estudos nas bases de dados Public Medline, Portal de Periódicos da Coordenação de Aperfeiçoamento de Pessoal de Nível Superior, Cumulative Index to Nursing and Allied Health Literature e TRIP DATABASE, pesquisadas em Abril de 2020. Resultados: Dos 12 estudos incluídos foram abordados os estressores percebidos pelos profissionais de saúde, as repercussões negativas e implicações psicológicas e os fatores de suporte para redução dos estressores. Conclusão: conclui-se que a avaliação da sobrecarga psíquica e a implementação de medidas de suporte aos profissionais de saúde faz parte da complexa rede de ações que determinam o êxito no enfrentamento à COVID-19 nos serviços de saúde

Objective: to identify negative repercussions and the psychological impact on health professionals who work in the care of patients with coronavirus and strategies to minimize its effects. Method: integrative review of publications from 2010 to 2020, in the Public Medline databases, Journal Portal of the Coordination for the Improvement of Higher Education Personnel, Cumulative Index to Nursing and Allied Health Literature and TRIP DATABASE. Results: the analysis of 12 studies related to psychological impacts on workers resulted in three categories: perceived stressors; negative repercussions and psychological implications; and supporting factors for reducing stressors. Conclusion: the coronavirus pandemic can cause psychological distress and the results indicate the need for attention to the mental health of health workers. Assessing psychic overload, offering psychological support and implementing protocols for a safe environment make up the complex network of actions that determine success in coping with COVID-19

Objetivo: identificar repercusiones negativas y el impacto psicológico en los profesionales de la salud que trabajan en la atención de pacientes con coronavirus y estrategias para minimizar sus efectos. Método: revisión integradora de publicaciones de 2010 a 2020, en las bases de datos Public Medline, Portal de Publicaciones Periódicas - Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Cumulative Index to Nursing and Allied Health Literature y TRIP DATABASE. Resultados: el análisis de 12 estudios relacionados con el impacto psicológico en los trabajadores resultó en tres categorías: factores de estrés percibido; repercusiones negativas e implicaciones psicológicas; y factores para la reducción de los estresantes. Conclusión: la pandemia por el coronavirus puede causar sufrimiento psicológico y los resultados indican la necesidad de prestar atención a la salud mental de trabajadores de la salud. evaluación de la sobrecarga psicológica, ofrecimiento de apoyo psicológico y la puesta en marcha de protocolos para un entorno seguro, conforman el complejo entramado de acciones que determinan el éxito a la hora de afrontar el COVID-19

Low-power laser alters mRNA levels from DNA repair genes in acute lung injury induced by sepsis in Wistar rats.

Acute lung injury (ALI) is defined as respiratory failure syndrome, in which the pathogenesis could occur from sepsis making it a life-threatening disease by uncontrolled hyperinflammatory responses. A possible treatment for ALI is the use of low-power infrared lasers (LPIL), whose therapeutical effects depend on wavelength, power, fluence, and emission mode. The evaluation mRNA levels of repair gene related to oxidative damage after exposure to LPIL could provide important information about the modulation of genes as treatment for ALI. Thus, the aim of this study was to evaluate the mRNA levels from OGG1, APEX1, ERCC2, and ERCC1 genes in lung tissue from Wistar rats affected by ALI and after exposure to LPIL (808 nm; 100 mW). Adult male Wistar rats (n = 30) were randomized into six groups (n = 5, for each group): control, 10 J/cm2 (2 J), 20 J/cm2 (5 J), ALI, ALI + LPIL 10 J/cm2 and ALI + LPIL 20 J/cm2. ALI was induced by intraperitoneal E. coli lipopolysaccharide injection (10 mg/kg). Lungs were removed, and samples were withdrawn for total RNA extraction, cDNA synthesis, and mRNA levels were evaluated by RT-qPCR. Data normality was verified by Kolmogorov-Smirnov, comparisons among groups were by Student's t test, Mann-Whitney test, one-way ANOVA, Kruskal-Wallis followed by post-tests. Data showed that OGG1 (0.39 ± 0.10), ERCC2 (0.67 ± 0.24), and ERCC1 (0.60 ± 0.19) mRNA levels are reduced in ALI group when compared with the control group (1.00 ± 0.07, 1.03 ± 0.25, 1.01 ± 0.16, respectively) and, after LPIL, mRNA relative levels from DNA repair genes are altered when compared to non-exposed ALI group. Our research shows that ALI alter mRNA levels from genes related to base and nucleotide excision repair genes, suggesting that DNA repair is part of cell response to sepsis, and that photobiomodulation could modulate the mRNA levels from these genes in lung tissue.